Dissolved inorganic carbon (DIC) fixation and dissolved organic carbon (DOC) release measurements of marine nitrifier cultures grown under different culture conditions from 2020-12-14 to 2021-08-13 (NCEI Accession 0292204)
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title: Dissolved inorganic carbon (DIC) fixation and dissolved organic carbon (DOC) release measurements of marine nitrifier cultures grown under different culture conditions from 2020-12-14 to 2021-08-13 (NCEI Accession 0292204)
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abstract: This dataset contains biological, chemical, physical, and survey - biological data collected from 2020-12-14 to 2021-08-13. These data include 14C, abundance, dissolved inorganic Carbon, dissolved organic Carbon, growth, particulate organic Carbon (POC), taxon, and water temperature. The instruments used to collect these data include Liquid Scintillation Counter. These data were collected by Alyson E. Santoro of University of California-Santa Barbara as part of the "Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories (O2 Min Metalloenzyme)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-03-17. The following is the text of the dataset description provided by BCO-DMO: Nitrifier C fixation release Dataset Description: Acquisition Description: DIC fixation was measured via the incorporation of [ 14 C]-bicarbonate as previously described (Herndl et al. 2005) with modifications. [ 14 C]-bicarbonate (specific activity 56 mCi mmol -1 /2.072 x 10 9 Bq mmol -1 , Perkin Elmer) was added to 5 mL of culture (between 6 and 65 µCi were added depending on the activity of the culture). For every culture condition, at least three replicate live samples and one formaldehyde-fixed blank (3% v/v) were incubated in temperature-controlled incubators in the dark. Parallel incubations without [ 14 C]-tracer additions were used to determine cell abundance and nitrite concentration. Incubations were terminated by adding formaldehyde (3% v/v) to 5 mL of sample. After 30-60 min, every sample was individually filtered onto 25 mm, 0.2 µm pore size polycarbonate filters (Millipore) and rinsed with 0.5 mL of artificial seawater using a glass filtration set (Millipore). The individual filtrates (5.5 mL per sample) were collected and transferred to scintillation vials to determine the fraction of [ 14 C]-dissolved organic carbon ([ 14 C]-DOC). Excess [ 14 C]-bicarbonate from the filters was removed by exposing them to fumes of concentrated HCl (37 %) for 24 h. The filters were transferred to scintillation vials and 10 mL of scintillation cocktail (Ultima Gold, Perkin Elmer) was added. The filtrates were acidified to pH ∼2 with HCl (25 %) as previously described (Marañón et al. 2004), and filtrates were kept for 24 h in open scintillation vials placed on an orbital shaker before 10 mL scintillation cocktail was added to each vial. Samples were shaken for ca. 30 sec and incubated in the dark for at least 24 hours prior to counting the disintegrations per minute (DPM) in a scintillation counter (Beckman Coulter LS6500) for 15 min. Total radioactivity measurements were performed to verify added [ 14 C]-bicarbonate concentrations by pipetting 100µl of sample into scintillation vials containing 400µl beta-phenylethylamine (to prevent outgassing of 14 CO 2 ). Scintillation cocktail was added, vials were shaken for ca. 30 sec and immediately measured in the scintillation counter. A detailed description of materials and methods can be found in Bayer et al. 2022.
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title: Bayer, B., Santoro, A. E. (2022) Dissolved inorganic carbon (DIC) fixation and dissolved organic carbon (DOC) release measurements of marine nitrifier cultures grown under different culture conditions. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-03-09. https://doi.org/10.26008/1912/bco-dmo.870832.1
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code: https://doi.org/10.26008/1912/bco-dmo.870832.1
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protocol: HTTPS
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description: originator dataset
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Anchor: http://lod.bco-dmo.org/id/person/555313 Alyson E. Santoro
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1444 University of California - Santa Barbara (UCSB)
contactInfo: (CI_Contact)
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name: UC Santa Barbara website
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1444 University of California-Santa Barbara
contactInfo: (CI_Contact)
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protocol: HTTP
name: UC Santa Barbara website
description: Institution web page
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protocol: HTTP
name: Biological and Chemical Oceanography Data Management Office website
description: Institution web page
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topicCategory: (MD_TopicCategoryCode) oceans
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northBoundLatitude: 34.413962
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extent:
TimePeriod:
beginPosition: 2020-12-14
endPosition: 2021-08-13
supplementalInformation: (missing)
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distributionInfo: (MD_Distribution)
distributor: (MD_Distributor)
distributorContact: (CI_ResponsibleParty)
organisationName:
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phone: (CI_Telephone)
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address: (CI_Address)
electronicMailAddress: NCEI.Info@noaa.gov
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distributionOrderProcess: (MD_StandardOrderProcess)
fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
distributorFormat: (MD_Format)
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applicationProfile: Any FTP client
name: FTP
description: These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
function: (CI_OnLineFunctionCode) download
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dataQualityInfo: (DQ_DataQuality)
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description: NCEI Accession 0292204 v1.1 was published.
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function: (CI_OnLineFunctionCode) download
role: (inapplicable)
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metadataMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
maintenanceNote: Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) custodian
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acquisitionInformation: (MI_AcquisitionInformation)
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: scintillation counter
type: scintillation counter
description: measures ionizing radiation A scintillation counter is an instrument for detecting and measuring ionizing radiation by using the excitation effect of incident radiation on a scintillating material, and detecting the resultant light pulses.
