Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019 (NCEI Accession 0291647)
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title: Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019 (NCEI Accession 0291647)
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abstract: This dataset contains chemical and physical data collected on R/V Atlantis and R/V Falkor during cruises AT42-05 and FK190211 from 2018-11-01 to 2019-03-01. These data include depth, methane, and water temperature. The instruments used to collect these data include Gas Chromatograph and Liquid Scintillation Counter. These data were collected by Samantha B. Joye of University of Georgia and Andreas Teske of University of North Carolina at Chapel Hill as part of the "Collaborative Research: Microbial Carbon cycling and its interactions with Sulfur and Nitrogen transformations in Guaymas Basin hydrothermal sediments (Guaymas Basin Interactions)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-03-17. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019.
purpose: This dataset is available to the public for a wide variety of uses including scientific research and analysis.
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title: Joye, S. B., Teske, A. (2023) Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2023-07-23. https://doi.org/10.26008/1912/bco-dmo.821587.2
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Anchor: http://lod.bco-dmo.org/id/person/51421 Samantha B. Joye
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1786 University of Georgia (UGA)
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Anchor: http://lod.bco-dmo.org/id/person/51412 Andreas Teske
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/738 University of North Carolina - Chapel Hill (UNC)
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Anchor: http://lod.bco-dmo.org/id/affiliation/191 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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beginPosition: 2018-11-01
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supplementalInformation: Acquisition Description: Sampling and analytical procedures: CH4: Samples were collected by filling 160 ml glass serum vials with seawater, stoppering them headspace-free with butyl chloride stoppers, and crimp sealing them. Samples were killed by injecting 0.5 ml 8 M NaOH. Ten ml killed sample was then displaced with a ten ml UHP nitrogen gas headspace. Samples were stored at room temperature until analysis. At the time of analysis, an additional ten ml of nitrogen was added to the vials. Vials were shaken for 2 min before headspace was sampled and injected into a GC FID (Shimadzu GC 2014, Alltech Carbosphere 80/100 6’x1/8” column). This method is adapted from Magen, 2014 (A simple headspace equilibration method for measuring dissolved methane. Limnol. Oceanogr. Methods 12(9): 637-650). MOx [data in both cruises]: Four technical replicates were collected from each sampled depth by filling 16.6 mL Hungate tubes headspace-free and sealing with exetainer septa. Tubes were stored at 4 C until injection. One of the four replicates was killed by displacing 1.6 ml sample with 1.6 ml 37% formalin. All samples, including the killed abiotic control, were injected with ~2000000 dpm tritiated methane tracer. Samples were incubated at near-in situ temperature for 1-4 days. Immediately before incubation termination, a 100 ul subsample was removed from each tube and measured on a liquid scintillation counter to determine total dissolved 3H-methane addition. Incubations were terminated by killing with 1.6 ml 37% formalin and purging with air for 1 hour to remove remaining tracer. A 5 ml aliquot of each killed replicate was measured on a liquid scintillation counter to determine the ratio of tracer turnover. The turnover ratio was multiplied by the measured in situ methane concentration to obtain the MOx rate. Killed controls were subtracted and the replicates averaged to obtain the final rate number. See Rogener, 2018 (Long-term impact of the Deepwater Horizon oil well blowout on methane oxidation dynamics in the northern Gulf of Mexico. Elem Sci Anth, 6(1), 73).
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DateTime: 2024-04-21T22:31:35Z
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acquisitionInformation: (MI_AcquisitionInformation)
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description: Gas, ion, liquid or other chromatograph
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code: scintillation counter
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description: measures ionizing radiation A scintillation counter is an instrument for detecting and measuring ionizing radiation by using the excitation effect of incident radiation on a scintillating material, and detecting the resultant light pulses.
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code: R/V Atlantis
description: Flag: United States of America Vessel Type: Research Vessel (Oceanographic) DOB: Mar 1997 IMO: 9105798 AS OF 3/3/1997 - WHOI https://www.whoi.edu/what-we-do/explore/ships/ships-atlantis/
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code: R/V Falkor
description: Updated information from ICES for the vessel Falkor from CAYMAN ISLANDS. Name : Falkor Flag : CAYMAN ISLANDS - KY (ISO Country Code) Call Sign : ZCYL5 Title : R/V Platform Class : 31 Research vessel IMO : 7928677 Commissioned Date : 2012 Current Length : 82.9 Built : 1981 Previous Name (Notes): Seefalke Notes : MMSI 319005600 Additional Information Sources: - http://www.schmidtocean.org/story/show/47 - http://www.vesseltracker.com/en/Ships/Falkor-7928677.html General Comments: Confirmed in Miramar Submitted to ICES on 2013-10-24. Confirmed by NOAA on 2013-12-04.
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