Seagrass metrics from from seagrass wasting disease mesocosm experiments conducted at Bodega Marine Laboratory from July-September 2015 (NCEI Accession 0291617)
(MI_Metadata)
fileIdentifier: gov.noaa.nodc:0291617
language:
LanguageCode: eng
characterSet: (MD_CharacterSetCode) utf8
parentIdentifier: gov.noaa.nodc:BCO-DMO
hierarchyLevel: (MD_ScopeCode) dataset
hierarchyLevelName: granule
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
address: (CI_Address)
electronicMailAddress: ncei.info@noaa.gov
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/contact
protocol: HTTPS
name: NOAA Contact Information
description: Information for contacts at NCEI.
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) custodian
dateStamp:
DateTime: 2024-06-26T18:45:28Z
metadataStandardName: ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
metadataStandardVersion: ISO 19115-2:2009(E)
return to top
identificationInfo: (MD_DataIdentification)
citation: (CI_Citation)
title: Seagrass metrics from from seagrass wasting disease mesocosm experiments conducted at Bodega Marine Laboratory from July-September 2015 (NCEI Accession 0291617)
date: (CI_Date)
date: 2024-04-21
dateType: (CI_DateTypeCode) publication
edition: (inapplicable)
identifier: (MD_Identifier)
authority: (CI_Citation)
title: NOAA National Centers for Environmental Information
date: (inapplicable)
code:
Anchor: NCEI Dataset ID gov.noaa.nodc:0291617
identifier: (MD_Identifier)
authority: (CI_Citation)
title: NCEI Archive Management System
date: (inapplicable)
code:
Anchor: NCEI Accession ID 0291617
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
address: (CI_Address)
electronicMailAddress: ncei.info@noaa.gov
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/contact
protocol: HTTPS
name: NCEI Contact Information
description: Information for contacts at NCEI.
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) publisher
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://bcodmo.org
protocol: HTTP
name: Biological and Chemical Oceanography Data Management Office website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) resourceProvider
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/522929 A. Randall Hughes
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/522929
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/881979 Katherine DuBois
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California - Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/881979
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/881977 Melissa Kardish
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/715 University of California - Davis; Bodega Marine Laboratory (BML)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/881977
protocol: HTTP
name: Bodega Marine Laboratory website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/851053 Forest Schenck
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/851053
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/518660 John J. Stachowicz
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California - Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/518660
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: http://lod.bco-dmo.org/id/affiliation/191 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/affiliation/191
protocol: HTTP
name: website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) pointOfContact
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/522929 Hughes, A. Randall
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/522929
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/881979 DuBois, Katherine
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California-Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/881979
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/881977 Kardish, Melissa
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/715 University of California-Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/881977
protocol: HTTP
name: Bodega Marine Laboratory website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/851053 Schenck, Forest
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/851053
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/518660 Stachowicz, John J.
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California-Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/518660
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California - Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://www.ucdavis.edu
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) contributor
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://www.northeastern.edu
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) contributor
presentationForm: (CI_PresentationFormCode) tableDigital
abstract: This dataset contains biological data collected from 2015-07-01 to 2015-09-14. These data include growth and temp_incub. The instruments used to collect these data include Aquarium, Aquarium chiller, Homogenizer, Immersion heater, and qPCR Thermal Cycler. These data were collected by A. Randall Hughes and Forest Schenck of Northeastern University and John J. Stachowicz, Katherine DuBois, and Melissa Kardish of University of California-Davis as part of the "CAREER: Linking genetic diversity, population density, and disease prevalence in seagrass and oyster ecosystems (Seagrass and Oyster Ecosystems)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-11-22. The following is the text of the dataset description provided by BCO-DMO: Acquisition Description: Mesocosm experiment. We used a substitutive design to test the effects of eelgrass genotypic identity (eight genotypes), diversity (monocultures of 1 genotype vs. polycultures of 4 genotypes), and temperature (ambient or + 3.2° C) on the prevalence and intensity of the wasting disease parasite Labyrinthula over eight weeks in an array of flow-through 120-Liter mesocosms at the Bodega Marine Laboratory in Bodega Bay, CA. In July 2015, we created ten unique polyculture combinations of four genotypes (4 genotypes per experimental pot) randomly drawn from a pool of eight genotypes; all eight genotypes were also grown in monoculture (1 genotype per pot). We filled pots (8.9 x 8.9 cm) with coarsely sieved sediment collected from Bodega Harbor, and planted 4 shoots of eelgrass per pot, matching the lower range of average field densities reported for Bodega Harbor (Ha and Williams, 2018) to allow for growth during the experiment. Plants were originally collected in Bodega Harbor, CA in 2012, confirmed to be unique genotypes using 11 DNA microsatellite loci developed specifically for Zostera marina (Abbott et al. 2018), and propagated in separate flow through mesocosms at Bodega Marine Lab. We previously characterized traits of each genotype relating to growth rate, morphology, nutrient content, and chemical defense in common garden experiments at ambient temperature from July 2013 to August 2014 (Abbott et al., 2018). We selected the 8 genotypes used in this experiment to encompass the range of trait values determined for this population of eelgrass measured when the common garden was experiencing marine heatwave conditions (DuBois et al., 2019). We assigned ten pots -- two unique polyculture combinations and each of the eight monocultures -- to each of ten mesocosms, with five mesocosms per temperature treatment (see DuBois et al. 2020 for a diagram of the experimental set up). All mesocosms received sand-filtered flow-through seawater at a rate of approximately 0.8-1.0 liters per minute (L min -1 ). We allowed the plants to acclimate for one month prior to initiating the temperature treatments. We maintained an ambient temperature treatment by cooling flow-through seawater in a head tank by approximately 1˚C using an Aqua Logic Delta Star in-line titanium chiller. Seawater in the elevated temperature treatment was raised approximately 3˚C above the ambient treatment in a separate header tank using Process Technologies titanium immersion heaters. This level of warming mimicked the 2014 and 2015 extreme warming events in the Northern Pacific, where the mass of unusually warm water called “The Blob” raised summer ocean temperatures three standard deviations above the long-term average (Sanford et al., 2019). At the end of the experiment (10 weeks), we estimated lesion percent cover of the third rank leaf of the terminal shoot of each transplant (i.e., focal leaf) to measure the signs of wasting disease (Burdick et al., 1993). We recorded lesions as either absent, <1%, <10%, or ≥10% cover. When lesions were ≥10% cover, we also recorded a numerical estimate of lesion percent cover. We then collected and preserved the top half of the focal leaf in individual plastic bags sealed with 30 milliliters of silica (Flower Drying Art Silica Gel; Activa) for subsequent DNA extraction and quantitative PCR to estimate Labyrinthula zosterae cells as a proxy for infection (Bergmann et al., 2011; Bockelmann et al., 2013; Groner et al., 2021). At one-month intervals over the course of the experiment, we measured leaf growth rate of the terminal shoot of each transplant using the “hole-punch” method (Williams and Ruckelshaus, 1993). Labyrinthula zosterae infection can be affected by plant defenses (Steele et al., 2005; Trevathan-Tackett et al., 2015) and these defenses may trade off with plant growth rate, resulting in a positive relationship between growth rate and infection. Alternatively, L. zosterae infection can result in reduced leaf growth rate (Graham et al., 2021), leading to a negative relationship. L. zosterae prevalence can also be affected by plant size (Groner et al., 2016), due to greater leaf-to-leaf contact and resulting increased parasite transmission (Muehlstein, 1992), so we measured the length of the focal leaf of each transplant at the end of the experiment. Labyrinthula zosterae DNA extraction and quantitative PCR Assay We extracted L. zosterae DNA from dried leaf tissue using Omega Bio-Tek E.Z. Tissue DNA extraction kits at the Northeastern University Marine Science Center in Nahant, MA. For each sample, we separated the dried leaf tissue into 2-16 mg subsamples and homogenized the tissue in a ball mill (Retsch, Germany) at a frequency of 30 Hz for 5 min (Bockelmann et al. 2013). We lysed ground subsamples individually following the manufacturer’s instructions and added 1 microliter of 500 ng per microliter (ng*uL -1 ) salmon sperm DNA solution (Invitrogen, USA) to the first subsample of each sample immediately before recombining all subsamples in the spin columns. Salmon sperm DNA was added to enhance extraction efficiency and ensure that even low amounts of target DNA are carried through the filter absorption steps (Bockelmann et al., 2013). We eluted all DNA extractions into 100 uL. Following elution, we used Zymo OneStep-96 PCR Inhibitor Removal kits to clean 50 uL sub-samples of each DNA extraction following the manufacturers instructions. We stored cleaned DNA extractions at -20˚C prior to quantitative PCR. We used a TaqMan quantitative PCR (qPCR) assay with a forward primer: TTGAACGTAACATT-CGACTTTCGT, reverse primer: ACGCATGAAGCGGTCTTCTT, and MBG probe: TGGACGAGTGTGTTTTG that carries the fluorescence label 6-Fam at the 5’ end and the dark quencher FHQ at the 3’ end (Bio-Rad, USA) developed specifically for L. zosterae (Bockelmann et al., 2013, Bergmann et al., 2011). We made up qPCR reactions to a 10 uL reaction volume using standard conditions recommended by the manufacturer: 5 uL SsoAdvanced TM Universal Probes Supermix 2x (Bio-Rad, USA), 1 uL template DNA, 0.4 uL 4:1 Primer:Probe Mix (final concentrations of 400 nM forward primer, 400 nM reverse primer, 100 nM probe), and 3.6 uL Milli-Q H 2 O (Thermofisher, USA). Reactions were run on a CFX96 Real-Time System (Bio-Rad, USA) using the following thermo-cycling program: 3 min at 95˚C, followed by 40 cycles of 15 sec at 95˚C and 1 min at 60˚C. We tested all samples in duplicate and if replicates differed by greater than one cycle threshold (Ct), reactions were rerun in triplicate. We only used the data from reactions in analyses when replicates fell within one Ct. Our lowest detection was 1.76 copies per reaction or 0.15 cells per extraction. We ran each 96-well plate of qPCR reactions with a set of nine standards: a dilution series of gBlock Gene Fragments (Integrated DNA Technologies, USA) designed based on the highly conserved sequence of the 5.8s ribosomal RNA gene of L. zosterae known as internal transcribed spacer 1 (ITS) targeted by the TaqMan qPCR assay; an L. zosterae cell standard consisting of a sample of DNA extracted from a know quantity of pathogenic L. zosterae cells; and an inhibition control consisting of a half volume of L. zosterae cell standard and a half volume of a haphazardly selected sample. We ran a total of 31 96-well plates of qPCR reactions with a mean efficiency of 97.4% ± 4.3 and R 2 0.996 ± 0.004. To convert Ct values to to L. zosterae cell numbers, four equations were used. The equations and details are captured in the Supplemental Files section of this metadata within the file titled, "Equations for conversion of Ct values to Labyrinthula zosterae cell numbers." We used a pure culture of the pathogenic L. zosterae isolate 316b provided by D. Martin in 2015 to make our L. zosterae cell standard (Martin et al., 2016; GenBank: KU559372.1). We cultured L. zosterae cells on serum seawater agar media (Muehlstein et al., 1991). We scraped cells from an actively growing edge of L. zosterae culture into serum seawater liquid media (D. Martin pers. com.). We mixed the liquid media + L. zosterae cell slurry vigorously on a bench top vortex for 30 sec and aliquoted immediately into three replicate subsamples for cell counts and extraction. In order to break up cell clumps for ease of counting, we added Tween80 (Sigma-Aldrich, USA) to a final concentration of 1:100 into the two subsamples used for cell counts, and mixed for 30 sec. We counted cells of four replicate aliquots per subsample on a hemocytometer. We calculated cell concentration by averaging over all replicates. Prior to DNA extraction, we centrifuged the third replicate L. zosterae cell solution at 6,000 g for 10 min and drew off the supernatant without disturbing the cell pellet. We then added a ~4 mg section of dried healthy Zostera. marina tissue to the cell pellet to account for possible interference of Zostera marina compounds in the extraction process. To extract L. zosterae DNA, we followed the DNA extraction and inhibitor removal protocols outlined above. We designed the gBlock double stranded DNA fragments (Integrated DNA Technologies, USA) using published sequences of the ITS region of the L. zosterae genome (GenBank: JN121409-13). 5’-CTGTGATCTCTGAAAATACTTGTTT (1) TTGAACGTAACATTCGACTTTCGT CGATT TTG (2) TGGACGAGTGTGTTTTGT AAACCTACCC (3) AAGAAGACCGCTTCATGCGT GTCGCTGACTAATGAAACAAACAAA-3’ The gBlock fragment sequences were a total length of 130 bp (base pairs), which included target regions for the forward (1) and reverse (3) primers and the MGD probe (2), underlined above, as well as 25 base pairs of additional sequence on both the 5’ and 3’ ends to increase fragment stability. We diluted gBlock fragments in Milli-Q H 2 O (Thermofisher, USA) to seven concentrations: 2.24e 1 , 1.12e 2 , 5.61e 2 , 2.81e 3 , 1.40e 4 , 7.02e 4 , 7.02e 5 copies/µL and included this dilution series in each qPCR run as a standard curve (Bergmann et al., 2011). The range of the gBlock dilution curve: approx. 1-60,000 cells/extraction encompassed the range of most L. zosterae values observed in our samples: 0.15-450,000 cells/extraction or 1.84e 2 -5.52e 8 copies/extraction. Life Sciences Identifiers (LSID) for taxonomic names: Zostera marina (urn:lsid:marinespecies.org:taxname:145795) Labyrinthula zosterae (urn:lsid:marinespecies.org:taxname:395093) Labyrinthula (urn:lsid:marinespecies.org:taxname:119090)
purpose: This dataset is available to the public for a wide variety of uses including scientific research and analysis.
credit:
Anchor: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1652320 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1652320 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1652320
status: (MD_ProgressCode) completed
status: (MD_ProgressCode) historicalArchive
pointOfContact: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
address: (CI_Address)
electronicMailAddress: ncei.info@noaa.gov
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/contact
protocol: HTTPS
name: NCEI Contact Information
description: Information for contacts at NCEI.
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) pointOfContact
resourceMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
graphicOverview: (MD_BrowseGraphic)
fileName: https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0291617
fileDescription: Preview graphic
fileType: PNG
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/accession/0291617 0291617
thesaurusName: (CI_Citation)
title: NCEI ACCESSION NUMBER
date: (CI_Date)
date: 2022-11-22
dateType: (CI_DateTypeCode) publication
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/datatype/details/534 growth rate
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/datatype/details/172 INCUBATION - TEMPERATURE
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: NODC DATA TYPES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/insttype/details/182 PCR machine
type: (MD_KeywordTypeCode) instrument
thesaurusName: (CI_Citation)
title: NODC INSTRUMENT TYPES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/obstype/details/2 biological
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: NODC OBSERVATION TYPES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California - Davis
type: (MD_KeywordTypeCode) dataCentre
thesaurusName: (CI_Citation)
title: NODC COLLECTING INSTITUTION NAMES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office
type: (MD_KeywordTypeCode) dataCentre
thesaurusName: (CI_Citation)
title: NODC SUBMITTING INSTITUTION NAMES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword: oceanography
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: WMO_CategoryCode
date: (CI_Date)
date: 2012-09-15
dateType: (CI_DateTypeCode) publication
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://gcmd.earthdata.nasa.gov/kms/concept/13afda36-ea44-427c-a297-1fe58083b8f0 BCO-DMO > Biological and Chemical Oceanography Data Management Office
type: (MD_KeywordTypeCode) dataCentre
thesaurusName: (CI_Citation)
title: Global Change Master Directory (GCMD) Data Center Keywords
date: (CI_Date)
date: 2023
dateType: (CI_DateTypeCode) revision
edition: 18
citedResponsibleParty: (CI_ResponsibleParty)
organisationName: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
contactInfo: (CI_Contact)
address: (CI_Address)
city: Greenbelt
administrativeArea: MD
onlineResource: (CI_OnlineResource)
linkage: https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
protocol: HTTPS
name: GCMD Keyword Forum Page
description: Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 18. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) custodian
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/project/709942 CAREER: Linking genetic diversity, population density, and disease prevalence in seagrass and oyster ecosystems (Seagrass and Oyster Ecosystems)
type: (MD_KeywordTypeCode) project
thesaurusName: (CI_Citation)
title: BCO-DMO Standard Projects
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1652320 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1652320
type: (MD_KeywordTypeCode) project
thesaurusName: (CI_Citation)
title: Provider Funding Award Information
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/514901 cell_concentration
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/1070 date
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/1730 flag
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/512798 growth
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/730 latitude
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/1097 length
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/731 longitude
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/886 mass
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/639488 percent coverage
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/960 sample identification
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/1742 sample type
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/1992 tank
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/541490 temp_incub
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/489066 treatment
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: BCO-DMO Standard Parameters
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881991 bin
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/882005 cells
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/882003 cells_per_extraction
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/882004 cells_per_mg
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881994 diversity
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881990 end_date
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881996 genotype
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881998 growth_rate
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/882006 latitude
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881997 length
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881999 lesion
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/882001 lesion_cover
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/882000 lesion_group
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/882007 longitude
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/882002 mass
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881992 pot
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881993 shoot
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881989 start_date
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/881995 temperature
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: Originator Parameter Names
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/711 Aquarium
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/522982 Aquarium chiller
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/522984 Homogenizer
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/522981 Immersion heater
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/707569 qPCR Thermal Cycler
type: (MD_KeywordTypeCode) instrument
thesaurusName: (CI_Citation)
title: BCO-DMO Standard Instruments
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/522982 Aqua Logic Delta Star in-line titanium chiller
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/707569 Bio-Rad CFX96 Real-Time System
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/522981 Process Technologies titanium immersion heater
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/522984 Retsch Mixer Mill 400
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/711 flow through tanks
type: (MD_KeywordTypeCode) instrument
thesaurusName: (CI_Citation)
title: Originator Instrument Names
date: (inapplicable)
resourceConstraints: (MD_Constraints)
useLimitation: accessLevel: Public
resourceConstraints: (MD_LegalConstraints)
useConstraints: (MD_RestrictionCode) otherRestrictions
otherConstraints: Cite as: Hughes, A. Randall; DuBois, Katherine; Kardish, Melissa; Schenck, Forest; Stachowicz, John J. (2024). Seagrass metrics from from seagrass wasting disease mesocosm experiments conducted at Bodega Marine Laboratory from July-September 2015 (NCEI Accession 0291617). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291617. Accessed [date].
resourceConstraints: (MD_LegalConstraints)
useLimitation: Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
resourceConstraints: (MD_LegalConstraints)
accessConstraints: (MD_RestrictionCode) otherRestrictions
otherConstraints: Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
resourceConstraints: (MD_LegalConstraints)
useConstraints: (MD_RestrictionCode) license
otherConstraints:
Anchor: CC-BY-4.0 This dataset is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
otherConstraints:
Anchor: CC-BY-4.0 SPDX License: Creative Commons Attribution 4.0 International (CC-BY-4.0)
aggregationInfo: (MD_AggregateInformation)
aggregateDataSetName: (CI_Citation)
title: Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
date: (CI_Date)
date: 2009-12-01
dateType: (CI_DateTypeCode) publication
identifier: (MD_Identifier)
authority: (CI_Citation)
title: NOAA National Centers for Environmental Information
date: (inapplicable)
code: gov.noaa.nodc:BCO-DMO
citedResponsibleParty: (CI_ResponsibleParty)
positionName: (unknown)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/BCO-DMO
protocol: HTTPS
name: NCEI Collection
description: Navigate directly to the URL for data access and direct download.
function: (CI_OnLineFunctionCode) information
role: (unknown)
citedResponsibleParty: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) publisher
associationType: (DS_AssociationTypeCode) largerWorkCitation
initiativeType: (DS_InitiativeTypeCode) collection
aggregationInfo: (MD_AggregateInformation)
aggregateDataSetName: (CI_Citation)
title: Schenck, F., DuBois, K., Kardish, M., Stachowicz, J. J., Hughes, A. R. (2022) Seagrass metrics from from seagrass wasting disease mesocosm experiments conducted at Bodega Marine Laboratory from July-September 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-10-06. https://doi.org/10.26008/1912/bco-dmo.879749.1
date: (CI_Date)
date: 2022-10-06
dateType: (CI_DateTypeCode) publication
identifier: (MD_Identifier)
authority: (CI_Citation)
title: International DOI Foundation (IDF)
date: (inapplicable)
code: https://doi.org/10.26008/1912/bco-dmo.879749.1
citedResponsibleParty: (CI_ResponsibleParty)
positionName: (unknown)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://doi.org/10.26008/1912/bco-dmo.879749.1
protocol: HTTPS
name: https://doi.org/10.26008/1912/bco-dmo.879749.1
description: originator dataset
function: (CI_OnLineFunctionCode) download
role: (unknown)
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/522929 A. Randall Hughes
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/522929
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/881979 Katherine DuBois
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California - Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/881979
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/881977 Melissa Kardish
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California - Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/881977
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/851053 Forest Schenck
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/851053
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/518660 John J. Stachowicz
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California - Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/518660
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: http://lod.bco-dmo.org/id/affiliation/191 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/affiliation/191
protocol: HTTP
name: website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) pointOfContact
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/522929 Hughes, A. Randall
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/522929
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/881979 DuBois, Katherine
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California-Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/881979
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/881977 Kardish, Melissa
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California-Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/881977
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/851053 Schenck, Forest
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1894 Northeastern University
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/851053
protocol: HTTP
name: Northeastern University website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/518660 Stachowicz, John J.
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1445 University of California-Davis
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/518660
protocol: HTTP
name: UC Davis website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://bcodmo.org
protocol: HTTP
name: Biological and Chemical Oceanography Data Management Office website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) publisher
presentationForm: (CI_PresentationFormCode) tableDigital
associationType: (DS_AssociationTypeCode) crossReference
initiativeType: (DS_InitiativeTypeCode) collection
language:
LanguageCode: eng; USA
characterSet: (MD_CharacterSetCode) utf8
topicCategory: (MD_TopicCategoryCode) environment
topicCategory: (MD_TopicCategoryCode) oceans
extent: (EX_Extent)
geographicElement: (EX_GeographicBoundingBox)
westBoundLongitude: -123.066
eastBoundLongitude: -123.066
southBoundLatitude: 38.318
northBoundLatitude: 38.318
temporalElement: (EX_TemporalExtent)
extent:
TimePeriod:
beginPosition: 2015-07-01
endPosition: 2015-09-14
supplementalInformation: (missing)
return to top
distributionInfo: (MD_Distribution)
distributor: (MD_Distributor)
distributorContact: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
phone: (CI_Telephone)
voice: +1-301-713-3277
address: (CI_Address)
electronicMailAddress: NCEI.Info@noaa.gov
role: (CI_RoleCode) pointOfContact
distributionOrderProcess: (MD_StandardOrderProcess)
fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
distributorFormat: (MD_Format)
name: TSV
version: (unknown)
distributorTransferOptions: (MD_DigitalTransferOptions)
transferSize:
Real: 0.608
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/0291617
protocol: HTTPS
applicationProfile: Web browser
name: NCEI Dataset Landing Page
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/oas/291617
protocol: HTTPS
applicationProfile: Web browser
name: Descriptive Information
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/download/291617
protocol: HTTPS
applicationProfile: Web browser
name: HTTPS
description: Navigate directly to the URL for data access and direct download.
function: (CI_OnLineFunctionCode) download
onLine: (CI_OnlineResource)
linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0227/0291617/
protocol: FTP
applicationProfile: Any FTP client
name: FTP
description: These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
function: (CI_OnLineFunctionCode) download
return to top
dataQualityInfo: (DQ_DataQuality)
scope: (DQ_Scope)
level: (MD_ScopeCode) dataset
lineage: (LI_Lineage)
processStep: (LE_ProcessStep)
description: NCEI Accession 0291617 v1.1 was published.
dateTime:
DateTime: 2024-04-21T21:58:37Z
output: (LE_Source)
sourceCitation: (CI_Citation)
title: NCEI Accession 0291617 v1.1
date: (CI_Date)
date: (inapplicable)
dateType: (CI_DateTypeCode) publication
citedResponsibleParty: (CI_ResponsibleParty)
individualName: (inapplicable)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/0291617/1.1
protocol: HTTPS
name: NCEI Accession 0291617 v1.1
description: published 2024-04-21T21:58:37Z
function: (CI_OnLineFunctionCode) download
role: (inapplicable)
return to top
metadataMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
maintenanceNote: Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) custodian
return to top
acquisitionInformation: (MI_AcquisitionInformation)
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: PCR machine
type: PCR machine
description: thermal cycler, thermocycler, PCR machine, DNA amplifier The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). https://en.wikipedia.org/wiki/Thermal_cycler, 2017-05-02.