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title: Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project) on 2016-06-17 (NCEI Accession 0291615)
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abstract: This dataset contains biological, chemical, and survey - biological data collectedat Lewes, Delaware on 2016-06-17. These data include abundance, metabolic_enzyme, and respiration rate. The instruments used to collect these data include Hemocytometer, Microscope-Fluorescence, Optode, PCR Thermal Cycler, Spectrophotometer, and plate reader. These data were collected by Dr Mark E Warner of University of Delaware as part of the "Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts (AnemoneOA)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-08-16. The following is the text of the dataset description provided by BCO-DMO: Host-symbiont respiration related to symbiont density; anemones from Key Largo Dataset Description: Results of laboratory experiments examining the respiratory capacity in a natural population of the sea anemone Exaiptasia pallida . This dataset details the relationships between host and symbiont respiration per symbiont density. Respiratory demand was assessed by both whole organismal oxygen consumption as well as in vitro mitochondrial activity via the enzyme citrate synthase.
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beginPosition: 2016-06-17
endPosition: 2016-06-17
supplementalInformation: Acquisition Description: Full details of the experimental design are provided in Hawkins et al., 2016
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fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
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function: (CI_OnLineFunctionCode) download
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code: fluorescence microscope
type: fluorescence microscope
description: Light Stimulating Microscope An optical microscope used to study properties of substances using fluorescence. The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed, causing light of longer wavelengths to be emitted.
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description: thermal cycler, thermocycler, PCR machine, DNA amplifier The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). https://en.wikipedia.org/wiki/Thermal_cycler, 2017-05-02.
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description: spectrophotometer, optical spectrometer, spectrograph or spectroscope An optical spectrometer (spectrophotometer, spectrograph or spectroscope) is an instrument used to measure properties of light over a specific portion of the electromagnetic spectrum, typically used in spectroscopic analysis to identify materials. Butler, L. R. P.; Laqua, K. (1995). "Nomenclature, symbols, units and their usage in spectrochemical analysis-IX. Instrumentation for the spectral dispersion and isolation of optical radiation (IUPAC Recommendations 1995)". Pure Appl. Chem. IUPAC. 67 (10): 1725–1744. doi:10.1351/pac199567101725. A spectrometer is the general term for describing a combination of spectral apparatus with one or more detectors to measure the intensity of one or more spectral bands."
