Respiration rates and protein content of larvae from experiments investigating heat priming in Nematostella vectensis from 2023-05-08 to 2023-08-02 (NCEI Accession 0291613)
(MI_Metadata)
fileIdentifier: gov.noaa.nodc:0291613
language:
LanguageCode: eng
characterSet: (MD_CharacterSetCode) utf8
parentIdentifier: gov.noaa.nodc:BCO-DMO
hierarchyLevel: (MD_ScopeCode) dataset
hierarchyLevelName: granule
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
address: (CI_Address)
electronicMailAddress: ncei.info@noaa.gov
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/contact
protocol: HTTPS
name: NOAA Contact Information
description: Information for contacts at NCEI.
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) custodian
dateStamp:
DateTime: 2024-05-31T15:15:28Z
metadataStandardName: ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
metadataStandardVersion: ISO 19115-2:2009(E)
return to top
identificationInfo: (MD_DataIdentification)
citation: (CI_Citation)
title: Respiration rates and protein content of larvae from experiments investigating heat priming in Nematostella vectensis from 2023-05-08 to 2023-08-02 (NCEI Accession 0291613)
date: (CI_Date)
date: 2024-04-21
dateType: (CI_DateTypeCode) publication
edition: (inapplicable)
identifier: (MD_Identifier)
authority: (CI_Citation)
title: NOAA National Centers for Environmental Information
date: (inapplicable)
code:
Anchor: NCEI Dataset ID gov.noaa.nodc:0291613
identifier: (MD_Identifier)
authority: (CI_Citation)
title: NCEI Archive Management System
date: (inapplicable)
code:
Anchor: NCEI Accession ID 0291613
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
address: (CI_Address)
electronicMailAddress: ncei.info@noaa.gov
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/contact
protocol: HTTPS
name: NCEI Contact Information
description: Information for contacts at NCEI.
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) publisher
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://bcodmo.org
protocol: HTTP
name: Biological and Chemical Oceanography Data Management Office website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) resourceProvider
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/843349 Katie Barott
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1917 University of Pennsylvania (UPENN)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/843349
protocol: HTTP
name: University of Pennsylvania website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: http://lod.bco-dmo.org/id/affiliation/191 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/affiliation/191
protocol: HTTP
name: website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) pointOfContact
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/843349 Barott, Katie
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1917 University of Pennsylvania
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/843349
protocol: HTTP
name: University of Pennsylvania website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1917 University of Pennsylvania (UPENN)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://www.upenn.edu
protocol: HTTP
name: University of Pennsylvania website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) contributor
presentationForm: (CI_PresentationFormCode) tableDigital
abstract: This dataset contains biological and chemical data collectedat Kaneohe Bay, Oahu, HI; Heron Island, Queensland, Australia from 2023-05-08 to 2023-08-02. These data include oxygen respiration rates from in situ incubation and proteins. The instruments used to collect these data include Camera, Centrifuge, In-situ incubator, Microscope - Optical, Oxygen Microelectrode Sensor, Oxygen Sensor, Thermal Cycler, ruler, and ultrasonic cell disrupter (sonicator). These data were collected by Katie Barott of University of Pennsylvania as part of the "Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress (Coral Resilience)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2024-03-27. The following is the text of the dataset description provided by BCO-DMO: Heat priming in Nematostella vectensis: Respiration rates and protein content of larvae Dataset Description: This dataset includes data pertaining to the respiration rates and protein content of larvae. See related datasets for other results from these experiments. These data and results are published in Glass et al. (2023) (https://doi.org/10.7717/peerj.16574 ). Methods and Sampling: Summary: Development data were collected via visual inspection of animals under brightfield illumination; animals were scored according to developmental stage (planula or post-planula). Dose-response curve data for larval heat tolerance were collected by exposing animals to heat ramps peaking at temperatures between 39-43°C, followed by visual scoring of mortality after 48 hours. Heat shock protein 70 (HSP70) expression was determined via Western blotting according to standard protocols. Sizes of larvae and juveniles (body column lengths) were measured from calibrated images of animals collected via brightfield microscopy. Respiration rates were determined using a Presens SensorDish Reader. Protein content of larvae was determined via a Bradford assay according to standard protocols. The following methods are excerpted from Glass et al. (2023) (https://doi.org/10.7717/peerj.16574 ). Adult collection, culture, and sexual reproduction Adult Nematostella vectensis sea anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Following transport to the laboratory, anemones were kept in 12 parts per thousand (ppt) artificial seawater (1/3-ASW; Spectrum Brands, Madison, WI, USA) at 18 degrees Celsius (°C) in a dark incubator (Boekel Scientific, Feasterville Trevose, PA, USA). Animals were fed twice per week with Artemia nauplii (Brine Shrimp Direct, Ogden, UT, USA) with water changes occurring every 2 weeks for approximately 2.5 years. Spawning was induced using a standard method for N. vectensis (Hand & Uhlinger, 1992; Fritzenwanker & Technau, 2002; Stefanik, Friedman & Finnerty, 2013). As culture containers housed both male and female anemones, eggs were left to fertilize during spawning and then transferred to a plastic dish with ~25 milliliters (mL) new 1/3-ASW. Fertilized embryos were then held at 18 °C in the dark for 3 days. Priming treatment and larval culture At 3 days post-fertilization (DPF), 200 swimming planula larvae were pipetted into each of 15 conical tubes (15 mL), for a total of three replicate tubes of 200 larvae (N = 600 larvae priming temperature⁻¹) for each of the five priming temperature treatments: 18 °C (control), 24 °C, 30 °C, 35 °C, and 39 °C. Tubes were placed in water baths (Thermo Fisher Scientific, Waltham, MA, USA) set to each treatment temperature for 1 hour. Following the treatment period, tubes containing larvae were poured into petri dishes (15 mL capacity) and a partial water change was performed by aspirating and replacing ~7.5 mL (~50%) 1/3-ASW; dishes were then held at 18 °C in the dark for the remainder of the experiment. The day after the priming treatment, one dish of larvae primed at 39 °C displayed 100% mortality along with dense overgrowth of an unidentified microbe, and was therefore removed from the experiment. All other larvae were left unfed and in the same water for the remainder of the short-term experiment (11 days in total). For the long-term experiment, this entire procedure was repeated for a second cohort of larvae, which were held at 18 °C for 6 weeks following priming at 3 DPF. In both experiments, control animals were kept at 18 °C for the entire experiment duration, having gone through the priming treatment but without a change in temperature. Image collection and quantification of growth and development Each day following the priming treatment (4-11 DPF), dishes containing larvae were individually removed from the incubator and photographed in a single, haphazardly chosen region under a dissecting microscope (Leica MZ12; Leica, Wetzlar, Germany) with a camera attachment (Retiga R3 CCD), after which they were returned to the incubator. Each photograph contained at least 20 larvae, and microscope settings were unchanged between dishes; a ruler was also photographed each day using the same microscope settings. To quantify larval growth, images were analyzed in FIJI (Schindelin et al., 2012); the ruler image was used to set the scale, and then the line tool was used to individually measure the lengths along the longest axis of at least 20 larvae per photograph (N = 40-60 larvae priming temperature⁻¹ time point⁻¹). Next, developmental progression was quantified from the images by counting the number of larvae clearly past the planula stage (i.e., not a homogenous oval shape), which was divided by the total number of larvae in each image and then converted to a percentage of "post-planula" larvae (N = 17-129 larvae priming temperature⁻¹ time point⁻¹). Respiration and total protein measurements At 4, 7, and 11 DPF, 15 larvae per culture dish (N = 30-45 larvae priming temperature⁻¹ time point⁻¹) were transferred to a 24-well plate (N = 1 well dish⁻¹) with oxygen sensor spots (Loligo Systems, Viborg, Denmark). All wells were filled to capacity (80 microliters (μL)) with 1/3-ASW, and 3-4 wells were also filled with culture water without larvae to serve as controls. The plate was sealed with an adhesive plate cover and placed on a calibrated PreSens SensorDish Reader (Precision Sensing, Regensburg, Germany) at room temperature (~21 °C) under ambient lighting. The oxygen concentration (micromoles oxygen per liter (μmol O2 L⁻¹)) in each well was recorded every 15 seconds for 1 hour, during which conditions remained normoxic (>180 μmol O2 L⁻¹). Following respiration measurements, larvae in each well (N = 15 larvae) were transferred to 1.5 mL tubes, seawater was removed, and the tubes were frozen and stored at -80 °C. Tubes containing larvae were later thawed on ice, and 60 μL of 1x tris-NaCl-EDTA lysis buffer supplemented with dithiothreitol, protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA), and phosphatase inhibitors (Roche, Basel, Switzerland), were added to each tube. Next, larvae were lysed in a water bath sonicator (Diagenode UCD-200; Diagenode Inc., Denville, NJ, USA) at 4 °C for 5 min with a 30:60 seconds on:off cycle. Following sonication, tubes were centrifuged (1,500 × g for 5 min at 4 °C) and the protein concentrations of the supernatants were determined in triplicate using a Bradford assay with a bovine serum albumin standard curve. Due to defects in the plastic of the 1.5 mL tubes used for total protein assays, six of the 42 protein samples were lost during processing. However, at least one sample was processed for each time point and priming temperature combination, with the exception of 24 °C at 7 DPF. To determine respiration rates, the rate of oxygen consumption for each well was determined as the slope of a linear best-fit line of the oxygen level in the well over time, and the average rate for the control wells was subtracted from the wells containing larvae. The absolute values of the slopes were then converted to pmol O2 minute⁻¹ μg protein⁻¹ by multiplying by 1,000 and the volume of the wells (80 μL), and dividing by the total protein. Heat tolerance measurements Larval heat tolerance was determined at 4, 7, and 11 DPF using previously established methods (Rivera et al., 2021; Glass et al., 2023). Specifically, six larvae from each culture dish were exposed to each of the following peak temperatures: 39 °C, 40 °C, 41 °C, 42 °C, or 43 °C, yielding 2–3 dose-response curves (N = 30 larvae curve⁻¹) per priming temperature. Specifically, individual larvae were pipetted into wells of a 96-well PCR plate with 100 μL of 1/3-ASW, and plates were sealed with an adhesive plate cover to prevent evaporation during the assay. Thermocyclers (Thermo Fisher Scientific, Waltham, MA, USA) were used to generate the heat ramps, and were programmed as follows: (i) 1 min at 25 °C; (ii) 4 min at 30 °C; (iii) 4 min at 38 °C; (iv) 1 h at peak temperature (39-43 °C); (v) 4 min at 38 °C; (vi) 4 min at 30 °C; and (vii) infinite hold at 22 °C. Following completion of the heat ramp, the plates were uncovered and placed at 18 °C for 48 h, after which larvae were scored as dead (visible tissue lysis) or alive (no visual abnormalities; often swimming). For each dose-response curve, the proportion of larvae surviving at each peak temperature was calculated by dividing the number of larvae surviving by the total number of larvae treated. Data pertaining to proportion survival were used to calculate lethal temperature 50s (LT50s) for each dose-response curve as detailed below. Western blotting for heat shock protein 70 (HSP70) Following the extraction of proteins and quantification via a Bradford assay, equal amounts (micrograms (μg)) of protein in lysis buffer from each group of larvae collected at 11 DPF (N = 2-3 groups of 15 larvae priming temperature⁻¹) were combined with Laemlli buffer (Bio-Rad, Hercules, CA, USA), denatured at 70 °C for 15 minutes, and loaded at a target amount of 2.02 μg protein well⁻¹ into a 4-12% tris-glycine gel. Next, electrophoresis was performed for 30 minutes at 60 volts (V) followed by 1 hour at 120 V, and proteins were then transferred to a polyvinylidene fluoride membrane (100 V for 100 minutes at 4 °C). Following transfer, the membrane was blocked for 1 hour in blocking buffer (5% w/v bovine serum albumin in tris-buffered saline (TBS) with 0.1% v/v Tween-20 (TBST)) and incubated with 0.55 μg mL⁻¹ polyclonal antibodies for HSP70 (Novus Biologicals, Centennial, CO, USA), which were chosen based on the target epitope's ability to bind to all five known isoforms of N. vectensis HSP70 (Waller et al., 2018; Knighton et al., 2019). Next, the membrane was washed (3 × 10 minutes with TBST) and a secondary antibody (anti-rabbit IgG with horseradish peroxidase) was added for 1 hour before final washing (3 × 10 min with TBST followed by 1 × 10 min with TBS), treatment with chemiluminescence reagents (Thermo Fisher Scientific, Waltham, MA, USA), and imaging on an Amersham Imager 600 (General Electric, Boston, MA, USA). After initial imaging, the membrane was probed for β-tubulin using 2.5 μg mL⁻¹ monoclonal antibodies (Cell Signaling Technology, Danvers, MA, USA) and reimaged. Long-term growth and heat tolerance experiments In order to characterize the persistence of heat priming effects on growth and heat tolerance, a second cohort of larvae was produced 2 weeks after the initial spawning by the same adult population and exposed to the same priming treatment at 3 DPF as described above. Following priming, larvae were kept in culture through 6 weeks post-fertilization (WPF) at 18 °C in the dark, where they progressed to the juvenile stage and began to grow tentacles. Animals were fed twice per week (i.e., every 3-4 days) after settlement (~7 DPF) with homogenized Artemia nauplii . Specifically, 3 mL of live Artemia nauplii were homogenized in a 15 mL conical using a rotostator at 20,000 rpm for 10 seconds, and 200 μL of the resulting slurry was added to each culture dish. Within 2-3 hours after feeding, a partial water change was performed for each dish by aspirating and replacing 5-7 mL (33-50%) of 1/3-ASW. Images were collected weekly as described above to characterize long-term effects of priming on growth, and the number of tentacles possessed by each juvenile in the images was also quantified. After 6 weeks, juvenile heat tolerance was determined via a single-temperature heat challenge at 42 °C due to fewer juveniles in the long-term priming experiment vs the short-term experiment. Specifically, eight juveniles from each culture dish (N = 24 juveniles priming temperature⁻¹) were exposed to a heat ramp peaking at 42 °C for 1 hour then returned to 18 °C. While juveniles were intended to be monitored at intervals over 48 hours to generate dose-response curves over time, >60% mortality for all groups at 21 hours precluded this procedure, so the assay was concluded at that time. Finally, the number of surviving juveniles was divided by the number treated to obtain the proportion surviving.
purpose: This dataset is available to the public for a wide variety of uses including scientific research and analysis.
credit:
Anchor: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1923743 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1923743 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1923743
status: (MD_ProgressCode) completed
status: (MD_ProgressCode) historicalArchive
pointOfContact: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
address: (CI_Address)
electronicMailAddress: ncei.info@noaa.gov
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/contact
protocol: HTTPS
name: NCEI Contact Information
description: Information for contacts at NCEI.
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) pointOfContact
resourceMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
graphicOverview: (MD_BrowseGraphic)
fileName: https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0291613
fileDescription: Graphic not available.
fileType: PNG
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/accession/0291613 0291613
thesaurusName: (CI_Citation)
title: NCEI ACCESSION NUMBER
date: (CI_Date)
date: 2024-03-27
dateType: (CI_DateTypeCode) publication
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/datatype/details/50 BIOCHEMISTRY
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/datatype/details/685 respiration rates
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: NODC DATA TYPES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/insttype/details/125 camera
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/insttype/details/221 microscope
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/insttype/details/27 oxygen sensor
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/insttype/details/182 PCR machine
type: (MD_KeywordTypeCode) instrument
thesaurusName: (CI_Citation)
title: NODC INSTRUMENT TYPES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/obstype/details/2 biological
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/obstype/details/3 chemical
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: NODC OBSERVATION TYPES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1917 University of Pennsylvania
type: (MD_KeywordTypeCode) dataCentre
thesaurusName: (CI_Citation)
title: NODC COLLECTING INSTITUTION NAMES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office
type: (MD_KeywordTypeCode) dataCentre
thesaurusName: (CI_Citation)
title: NODC SUBMITTING INSTITUTION NAMES THESAURUS
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword: oceanography
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: WMO_CategoryCode
date: (CI_Date)
date: 2012-09-15
dateType: (CI_DateTypeCode) publication
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://gcmd.earthdata.nasa.gov/kms/concept/13afda36-ea44-427c-a297-1fe58083b8f0 BCO-DMO > Biological and Chemical Oceanography Data Management Office
type: (MD_KeywordTypeCode) dataCentre
thesaurusName: (CI_Citation)
title: Global Change Master Directory (GCMD) Data Center Keywords
date: (CI_Date)
date: 2023
dateType: (CI_DateTypeCode) revision
edition: 18
citedResponsibleParty: (CI_ResponsibleParty)
organisationName: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
contactInfo: (CI_Contact)
address: (CI_Address)
city: Greenbelt
administrativeArea: MD
onlineResource: (CI_OnlineResource)
linkage: https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
protocol: HTTPS
name: GCMD Keyword Forum Page
description: Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 18. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) custodian
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/project/843347 Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress (Coral Resilience)
type: (MD_KeywordTypeCode) project
thesaurusName: (CI_Citation)
title: BCO-DMO Standard Projects
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1923743 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1923743
type: (MD_KeywordTypeCode) project
thesaurusName: (CI_Citation)
title: Provider Funding Award Information
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/1079 count
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/1620 oxygen respiration rates from in situ incubation
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/663611 proteins
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/960 sample identification
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/1838 time_elapsed
keyword:
Anchor: http://lod.bco-dmo.org/id/parameter/489066 treatment
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: BCO-DMO Standard Parameters
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://gcmd.earthdata.nasa.gov/kms/concept/f9cdf3ae-fe8b-4a19-a946-a8c8780d7894 EARTH SCIENCE > AGRICULTURE > ANIMAL SCIENCE > ANIMAL PHYSIOLOGY AND BIOCHEMISTRY
keyword:
Anchor: https://gcmd.earthdata.nasa.gov/kms/concept/29a64468-46a8-4dbc-955d-80b7b4cf9aaf EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > RESPIRATION RATE
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: Global Change Master Directory (GCMD) Science Keywords
date: (CI_Date)
date: 2023
dateType: (CI_DateTypeCode) revision
edition: 18
citedResponsibleParty: (CI_ResponsibleParty)
organisationName: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
contactInfo: (CI_Contact)
address: (CI_Address)
city: Greenbelt
administrativeArea: MD
onlineResource: (CI_OnlineResource)
linkage: https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
protocol: HTTPS
name: GCMD Keyword Forum Page
description: Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 18. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) custodian
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923695 Days_post_fertilization
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923694 Group
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923697 Number_of_larvae
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923693 Priming_temperature_C
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923699 Protein_ug
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923700 Protein_ug_per_larva
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923696 Respiration_nmol_O2_per_minute
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923698 Respiration_nmol_O2_per_minute_per_larva
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923701 Respiration_nmol_O2_per_minute_per_protein
keyword:
Anchor: http://lod.bco-dmo.org/id/dataset-parameter/923702 Respiration_pmol_O2_per_minute_per_protein
type: (MD_KeywordTypeCode) theme
thesaurusName: (CI_Citation)
title: Originator Parameter Names
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/520 Camera
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/629890 Centrifuge
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/494 In-situ incubator
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/708 Microscope - Optical
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/701 Oxygen Microelectrode Sensor
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/705 Oxygen Sensor
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/471582 Thermal Cycler
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/867857 ruler
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/528691 ultrasonic cell disrupter (sonicator)
type: (MD_KeywordTypeCode) instrument
thesaurusName: (CI_Citation)
title: BCO-DMO Standard Instruments
date: (inapplicable)
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: https://gcmd.earthdata.nasa.gov/kms/concept/33de4965-928a-45d5-acfc-79e006842431 CAMERA > CAMERA
keyword:
Anchor: https://gcmd.earthdata.nasa.gov/kms/concept/99f640d4-6b01-4646-b4e2-315885e01bf4 MICROSCOPES > MICROSCOPES
keyword:
Anchor: https://gcmd.earthdata.nasa.gov/kms/concept/fdcfecd6-9731-450e-94e8-5bc4c04e22a2 OXYGEN METERS > OXYGEN METERS
type: (MD_KeywordTypeCode) instrument
thesaurusName: (CI_Citation)
title: Global Change Master Directory (GCMD) Instrument Keywords
date: (CI_Date)
date: 2023
dateType: (CI_DateTypeCode) revision
edition: 18
citedResponsibleParty: (CI_ResponsibleParty)
organisationName: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
contactInfo: (CI_Contact)
address: (CI_Address)
city: Greenbelt
administrativeArea: MD
onlineResource: (CI_OnlineResource)
linkage: https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
protocol: HTTPS
name: GCMD Keyword Forum Page
description: Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 18. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) custodian
descriptiveKeywords: (MD_Keywords)
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/471582 MiniAmp thermal cyclers
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/701 PreSens SensorDish Reader
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/520 Retiga R3 microscope camera
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/629890 centrifuge
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/708 dissecting microscope (Leica MZ12; Leica, Wetzlar, Germany)
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/494 incubator (Boekel Scientific, Feasterville Trevose, PA, USA)
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/705 oxygen sensor spots (Loligo Systems, Viborg, Denmark)
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/867857 ruler
keyword:
Anchor: http://lod.bco-dmo.org/id/instrument/528691 water bath sonicator (Diagenode UCD-200; Diagenode Inc., Denville, NJ, USA)
type: (MD_KeywordTypeCode) instrument
thesaurusName: (CI_Citation)
title: Originator Instrument Names
date: (inapplicable)
resourceConstraints: (MD_Constraints)
useLimitation: accessLevel: Public
resourceConstraints: (MD_LegalConstraints)
useConstraints: (MD_RestrictionCode) otherRestrictions
otherConstraints: Cite as: Barott, Katie (2024). Respiration rates and protein content of larvae from experiments investigating heat priming in Nematostella vectensis from 2023-05-08 to 2023-08-02 (NCEI Accession 0291613). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291613. Accessed [date].
resourceConstraints: (MD_LegalConstraints)
useLimitation: Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
resourceConstraints: (MD_LegalConstraints)
accessConstraints: (MD_RestrictionCode) otherRestrictions
otherConstraints: Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
resourceConstraints: (MD_LegalConstraints)
useConstraints: (MD_RestrictionCode) license
otherConstraints:
Anchor: CC-BY-4.0 This dataset is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
otherConstraints:
Anchor: CC-BY-4.0 SPDX License: Creative Commons Attribution 4.0 International (CC-BY-4.0)
aggregationInfo: (MD_AggregateInformation)
aggregateDataSetName: (CI_Citation)
title: Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
date: (CI_Date)
date: 2009-12-01
dateType: (CI_DateTypeCode) publication
identifier: (MD_Identifier)
authority: (CI_Citation)
title: NOAA National Centers for Environmental Information
date: (inapplicable)
code: gov.noaa.nodc:BCO-DMO
citedResponsibleParty: (CI_ResponsibleParty)
positionName: (unknown)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/BCO-DMO
protocol: HTTPS
name: NCEI Collection
description: Navigate directly to the URL for data access and direct download.
function: (CI_OnLineFunctionCode) information
role: (unknown)
citedResponsibleParty: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) publisher
associationType: (DS_AssociationTypeCode) largerWorkCitation
initiativeType: (DS_InitiativeTypeCode) collection
aggregationInfo: (MD_AggregateInformation)
aggregateDataSetName: (CI_Citation)
title: Glass, B., Barott, K. (2024) Respiration rates and protein content of larvae from experiments investigating heat priming in Nematostella vectensis. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-27. https://doi.org/10.26008/1912/bco-dmo.923674.1
date: (CI_Date)
date: 2024-03-27
dateType: (CI_DateTypeCode) publication
identifier: (MD_Identifier)
authority: (CI_Citation)
title: International DOI Foundation (IDF)
date: (inapplicable)
code: https://doi.org/10.26008/1912/bco-dmo.923674.1
citedResponsibleParty: (CI_ResponsibleParty)
positionName: (unknown)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://doi.org/10.26008/1912/bco-dmo.923674.1
protocol: HTTPS
name: https://doi.org/10.26008/1912/bco-dmo.923674.1
description: originator dataset
function: (CI_OnLineFunctionCode) download
role: (unknown)
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/843349 Katie Barott
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1917 University of Pennsylvania (UPENN)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/843349
protocol: HTTP
name: University of Pennsylvania website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) principalInvestigator
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: http://lod.bco-dmo.org/id/affiliation/191 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/affiliation/191
protocol: HTTP
name: website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) pointOfContact
citedResponsibleParty: (CI_ResponsibleParty)
individualName:
Anchor: http://lod.bco-dmo.org/id/person/843349 Barott, Katie
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1917 University of Pennsylvania
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/843349
protocol: HTTP
name: University of Pennsylvania website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://bcodmo.org
protocol: HTTP
name: Biological and Chemical Oceanography Data Management Office website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) publisher
presentationForm: (CI_PresentationFormCode) tableDigital
associationType: (DS_AssociationTypeCode) crossReference
initiativeType: (DS_InitiativeTypeCode) collection
language:
LanguageCode: eng; USA
characterSet: (MD_CharacterSetCode) utf8
topicCategory: (MD_TopicCategoryCode) environment
topicCategory: (MD_TopicCategoryCode) oceans
extent: (EX_Extent)
description: Kaneohe Bay, Oahu, HI; Heron Island, Queensland, Australia
geographicElement: (EX_GeographicBoundingBox)
westBoundLongitude: (missing)
eastBoundLongitude: (missing)
southBoundLatitude: (missing)
northBoundLatitude: (missing)
temporalElement: (EX_TemporalExtent)
extent:
TimePeriod:
beginPosition: 2023-05-08
endPosition: 2023-08-02
supplementalInformation: (missing)
return to top
distributionInfo: (MD_Distribution)
distributor: (MD_Distributor)
distributorContact: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
phone: (CI_Telephone)
voice: +1-301-713-3277
address: (CI_Address)
electronicMailAddress: NCEI.Info@noaa.gov
role: (CI_RoleCode) pointOfContact
distributionOrderProcess: (MD_StandardOrderProcess)
fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
distributorFormat: (MD_Format)
name: CSV
version: (unknown)
distributorTransferOptions: (MD_DigitalTransferOptions)
transferSize:
Real: 0.228
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/0291613
protocol: HTTPS
applicationProfile: Web browser
name: NCEI Dataset Landing Page
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/oas/291613
protocol: HTTPS
applicationProfile: Web browser
name: Descriptive Information
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/download/291613
protocol: HTTPS
applicationProfile: Web browser
name: HTTPS
description: Navigate directly to the URL for data access and direct download.
function: (CI_OnLineFunctionCode) download
onLine: (CI_OnlineResource)
linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0227/0291613/
protocol: FTP
applicationProfile: Any FTP client
name: FTP
description: These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
function: (CI_OnLineFunctionCode) download
return to top
dataQualityInfo: (DQ_DataQuality)
scope: (DQ_Scope)
level: (MD_ScopeCode) dataset
lineage: (LI_Lineage)
processStep: (LE_ProcessStep)
description: NCEI Accession 0291613 v1.1 was published.
dateTime:
DateTime: 2024-04-21T21:53:08Z
output: (LE_Source)
sourceCitation: (CI_Citation)
title: NCEI Accession 0291613 v1.1
date: (CI_Date)
date: (inapplicable)
dateType: (CI_DateTypeCode) publication
citedResponsibleParty: (CI_ResponsibleParty)
individualName: (inapplicable)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/0291613/1.1
protocol: HTTPS
name: NCEI Accession 0291613 v1.1
description: published 2024-04-21T21:53:08Z
function: (CI_OnLineFunctionCode) download
role: (inapplicable)
return to top
metadataMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
maintenanceNote: Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) custodian
return to top
acquisitionInformation: (MI_AcquisitionInformation)
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: camera
type: camera
description: An instrument for recording images.
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: microscope
type: microscope
description: instrument is used in lab analyses A microscope is an instrument used to see objects that are too small for the naked eye.
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: oxygen sensor
type: oxygen sensor
description: Any instrument that measures oxygen content in a compound.
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: PCR machine
type: PCR machine
description: thermal cycler, thermocycler, PCR machine, DNA amplifier The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). https://en.wikipedia.org/wiki/Thermal_cycler, 2017-05-02.