Physiological observations of Euphausia pacifica after a ten-day acclimation to dissolved oxygen (DO) and pH conditions in two separate laboratory experiments on 2021-02-11 (NCEI Accession 0291549)
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title: Physiological observations of Euphausia pacifica after a ten-day acclimation to dissolved oxygen (DO) and pH conditions in two separate laboratory experiments on 2021-02-11 (NCEI Accession 0291549)
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abstract: This dataset contains biological data collected on R/V Clifford A. Barnes during cruises CB1073 and CB1078 in the Coastal Waters of Southeast Alaska and British Columbia on 2021-02-11. These data include respiration rate. The instruments used to collect these data include Dissolved Oxygen Sensor. These data were collected by Julie E. Keister of University of Washington as part of the "Consequences of hypoxia on food web linkages in a pelagic marine ecosystem (PelagicHypoxia)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-02-19. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Experiments done at: Friday Harbor Laboratories Ocean Acidification Environmental Laboratory NOAA Northwest Fisheries Science Center Mukilteo Research Station
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Anchor: http://lod.bco-dmo.org/id/affiliation/191 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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individualName:
Anchor: http://lod.bco-dmo.org/id/person/51330 Keister, Julie E.
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/984 University of Washington
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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language:
LanguageCode: eng; USA
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topicCategory: (MD_TopicCategoryCode) environment
topicCategory: (MD_TopicCategoryCode) oceans
extent: (EX_Extent)
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westBoundLongitude: -122.29
eastBoundLongitude: -122.29
southBoundLatitude: 48.01
northBoundLatitude: 48.01
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TimePeriod:
beginPosition: 2021-02-11
endPosition: 2021-02-11
supplementalInformation: Acquisition Description: Krill for both experiments were collected from surface waters (upper 50 m) at approximately 48.01, -122.29 using a 60-cm diameter Bongo frame equipped with black 335-μm mesh nets and non-filtering cod ends towed for <10 min. The contents of each cod end were immediately diluted in coolers of seawater and sorted to remove healthy euphausiids. For the pH experiment krill were collected on 23 July 2017 between 23:00-00:30, stored in 1-4 L containers at a concentration of 5 ind L-1, sealed with all air removed to eliminate splashing, and transported in coolers to the University of Washington Friday Harbor Laboratories (FHL) where they were sorted for healthy female E. pacifica (obvious ovary) and put in in experimental chambers between 06:00-08:00 on 24 July 2017. For the DO experiment krill were collected on 20 Sept 2017 between 20:00 and 21:15, krill were transported in coolers to the NOAA Mukilteo Research Station within an hour, sorted for healthy female E. pacifica (obvious ovary), and immediately put in experimental chambers. Krill were kept chilled (10-14 °C) at all times and were gently transferred using broad spoons to prevent damage. During experiments krill were fed Shellfish Diet 1800 (Reed Mariculture) twice per day and newly hatched Artemia salina (San Francisco Bay Brand) nauplii once per day. All physiological measurements were taken after 10 days of acclimation; individuals were flash frozen in liquid nitrogen after oxygen consumption was measured. Experimental acclimation to pH was conducted at the FHL Ocean Acidification Environmental Laboratory, using a flow-through system with pH controlled by CO 2 bubbling. Target pH levels were 8.0, 7.5, and 7.2: three flow-through systems were used, one per pH treatment. Within each system, conditioned seawater was delivered to eight flow-through boxes that each contained two 500-mL mesh-sided experimental chambers with one individual krill per chamber, for a total of 16 individuals per system. Experimental systems were kept covered in black plastic to reduce light. Temperature was set to 12 °C and oxygen was maintained at ambient levels through bubbling, but not monitored. Acclimation of krill to different oxygen levels in the laboratory was done at the NOAA Mukilteo Research Station in flow-through experimental systems with independent temperature, pH, and oxygen control. Target dissolved oxygen levels were 3, 5, and 9 mg DO L -1 : nine systems were used, three per DO treatment, with krill held individually in a mix of both 250- and 500-mL flow-through containers within the experimental systems. Temperature was maintained at 12 °C and pH at ~7.82; temperature, pH, and dissolved oxygen were monitored continuously.
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fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
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linkage: https://www.ncei.noaa.gov/archive/accession/0291549
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linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0226/0291549/
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function: (CI_OnLineFunctionCode) download
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description: NCEI Accession 0291549 v1.1 was published.
dateTime:
DateTime: 2024-04-21T15:51:44Z
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title: NCEI Accession 0291549 v1.1
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description: published 2024-04-21T15:51:44Z
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acquisitionInformation: (MI_AcquisitionInformation)
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code: oxygen sensor
type: oxygen sensor
description: Any instrument that measures oxygen content in a compound.
platform: (MI_Platform)
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code: R/V Clifford A. Barnes
description: R/V; owned by the National Science Foundation and operated under a Charter Party agreement by the School of Oceanography at the University of Washington as part of the UNOLS fleet.
instrument: (missing)
