The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017 (NCEI Accession 0291508)
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title: The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017 (NCEI Accession 0291508)
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abstract: This dataset contains biological and physical data collected from 2017-01-01 to 2018-10-31. These data include taxon_code and water temperature. The instruments used to collect these data include Turner Designs Fluorometer -10-AU. These data were collected by David A. Hutchins of University of Southern California as part of the "Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-04-27. The following is the text of the dataset description provided by BCO-DMO: Synechococcus thermal niche calcs Dataset Description: The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017.
purpose: This dataset is available to the public for a wide variety of uses including scientific research and analysis.
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title: Hutchins, D. A. (2019) The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017. Dataset version 2019-11-20. https://doi.org/10.26008/1912/bco-dmo.782308.1
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supplementalInformation: Acquisition Description: Natural seawater was enriched for photoautotrophs and split into multiple temperatures for two weeks. After the enrichment period, Synechococcus was isolated from each temperature. Each isolate's thermal niche (Thermal Performance Curve) was measured through a series of lab experiments and sequenced. Methods: Thermal niches were calculated by measuring each strain’s thermal performance curve. This was done by acclimating aliquots of each culture for two weeks to a broad range of temperatures between 9° and 33°. Temperatures >33° were added as needed for strains able to grow at these levels. This temperature range was chosen because it exceeds the current summer high and low temperatures in Narragansett Bay, and encompasses the projected warmer temperatures expected in coming decades. Strains were grown at each temperature in triplicate 8 ml borosilicate vials containing 5ml of F/2 medium. Biomass was recorded every two days using in vivo chlorophyll a fluorescence measured on a Turner AU-10 fluorometer (Turner Designs Inc., Sunnyvale, CA, USA), and growth rates and Eppley-Norberg thermal performance curves (Norberg 2004) were calculated in R (Team 2019) using the package growthTools (https://doi.org/10.5281/zenodo.3634918). Rare cultures containing contaminants (verified using fluorescence microscopy) were excluded from the dataset. In two strains for low temperature treatments, LA20 and LA27, after two weeks of acclimation no Synechococcus cells were observed in the culture, so the growth rate was set to zero for these cultures. The results of these thermal performance curves are hereafter referred to as a strain’s phenotype.
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name: FTP
description: These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
function: (CI_OnLineFunctionCode) download
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dataQualityInfo: (DQ_DataQuality)
scope: (DQ_Scope)
level: (MD_ScopeCode) dataset
lineage: (LI_Lineage)
processStep: (LE_ProcessStep)
description: NCEI Accession 0291508 v1.1 was published.
dateTime:
DateTime: 2024-04-21T06:09:55Z
output: (LE_Source)
sourceCitation: (CI_Citation)
title: NCEI Accession 0291508 v1.1
date: (CI_Date)
date: (inapplicable)
dateType: (CI_DateTypeCode) publication
citedResponsibleParty: (CI_ResponsibleParty)
individualName: (inapplicable)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/0291508/1.1
protocol: HTTPS
name: NCEI Accession 0291508 v1.1
description: published 2024-04-21T06:09:55Z
function: (CI_OnLineFunctionCode) download
role: (inapplicable)
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metadataMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
maintenanceNote: Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) custodian
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acquisitionInformation: (MI_AcquisitionInformation)
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: fluorometer
type: fluorometer
description: fluorometer
