Partitioning of carbon as a function of pCO2 and temperature during growth of Thalassiosira weissflogii from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Effects of High CO2 project) (NCEI Accession 0278550)
This dataset contains physical data collected at UCSB MSI Passow during deployment lab_UCSB_MSI_Passow from 2013-03-01 to 2013-05-31. These data include water temperature. The instruments used to collect these data include CHN Elemental Analyzer, Flow Injection Analyzer, LI-COR LI-840 NDIR Gas Analyzer, Shimadzu TOC-V Analyzer, and pH Sensor. These data were collected by Dr Craig Carlson, Dr Uta Passow, and Mark Brzezinski of University of California-Santa Barbara as part of the "Will high CO2 conditions affect production, partitioning and fate of organic matter? (OA - Effects of High CO2)" project and "Ocean Carbon and Biogeochemistry (OCB)" and "Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-08-28.
The following is the text of the dataset description provided by BCO-DMO:
Data Set #1A: Partitioning of carbon as a function of pCO2 and temperature during growth of Thalassiosira weissflogii
Dataset Description:
Experiments with the diatom Thalassiosira weissflogii (CCMP 1336) on impact of temperature and carbonate chemistry on carbon uptake and partitioning into particulate and dissolved organic matter
Experiments conducted in March and May 2013 in Santa Barbara California, Passow lab
Treatments: mulitfactorial analysis with 2 temperature treatments (15, 20C) and two ocean acidification treatments (400 and 1000 µatm)
Daily sampling after light cycle (14/10) was completed
See related publication Thaucher et al (20115).
The following is the text of the dataset description provided by BCO-DMO:
Data Set #1A: Partitioning of carbon as a function of pCO2 and temperature during growth of Thalassiosira weissflogii
Dataset Description:
Experiments with the diatom Thalassiosira weissflogii (CCMP 1336) on impact of temperature and carbonate chemistry on carbon uptake and partitioning into particulate and dissolved organic matter
Experiments conducted in March and May 2013 in Santa Barbara California, Passow lab
Treatments: mulitfactorial analysis with 2 temperature treatments (15, 20C) and two ocean acidification treatments (400 and 1000 µatm)
Daily sampling after light cycle (14/10) was completed
See related publication Thaucher et al (20115).
Dataset Citation
- Cite as: Passow, Uta; Brzezinski, Mark A.; Carlson, Craig (2023). Partitioning of carbon as a function of pCO2 and temperature during growth of Thalassiosira weissflogii from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Effects of High CO2 project) (NCEI Accession 0278550). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278550. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278550
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Time Period | 2013-03-01 to 2013-05-31 |
Spatial Bounding Box Coordinates |
West: -119.842
East: -119.842
South: 34.413
North: 34.413
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Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Methods: Two experiments, one with Thalassiosira weissflogii and one with Dacyliosolen fragilissimus were conducted. Replicates of each treatment (2 temperatures by 2 pCO2 conditions) were grown in eight 20 L gas-tight polyethylene bags. T. weissflogii and D. fragilissimus , respectively, were grown in artificial and natural seawater based, modified f/2 media. Initial nitrate addition was 15 µmol L-1 NO3 for both species, and 6 and 8 µmol L-1 PO4, and 16 and 50 µmol L-1 SiO3 for T. weissflogii and D. fragilissimus , respectively. Trace metals and vitamins were added according to f/8. Cultures were grown under a light / dark cycle of 14/10 hours at ~90 – 100 µE m-2 s-1 at 15 °C and 20 °C. Partial pressure levels of CO2 were set without bubbling to 400 and 1000 µatm for both temperatures; by appropriate addition of HCO3 and HCl. Prior to the experiments the diatoms were acclimatized to the respective target conditions growing semi-continuously in gas-tight polycarbonate bottles for at least a week. At the onset of the experiment bags were inoculated with ~1000 cells ml-1 for T. weissflogii and ~500 cells ml-1 for the larger D. fragilissimus . Daily sampling conducted immediately after the end of each light cycle, was continued for at least 6 days after NO3 depletion. For the determination of particulate organic carbon and nitrogen (POC and PON), samples were filtered onto precombusted (5 hours at 450 °C) glassfibre filters (Whatman, GF/F, 0.7 µm nominal poresize), dried at ~60 °C for 24 hours and analyzed on a CHN organic elemental analyzer (Control Equipment Corp., CEC 440HA). Samples for dissolved inorganic nitrate, nitrite, phosphate and silicate were filtered through 0.2 µm filters and measured on a flow injection analyzer (Lachat Instruments Div., QuikChem 8000). Samples for dissolved organic carbon (DOC) were gravity-filtered through precombusted GF/F filters, with the filtrate being collecting collected in acid-washed (HCl, 10%) and precombusted glass vials and frozen (at -20 °C). The analysis was carried out via high temperature combustion on a modified Shimadzu TOC-V analyzers. Dissolved inorganic carbon (DIC) was measured on a non-dispersive infrared (NDIR) analyzer. Samples were filtered through glassfibre filters (GF/F) and stored in gas-tight ~400 ml borosilicate bottles until analysis. pH (total scale) was measured spetrophotometrically at 25ºC (Thermo Scientific Genesys 105 VIS Spetrophotometer with a SPG 1A air-cooled single cell Peltier element), using m-cresol as an indicator dye. The dye was calibrated against certified reference material (A. Dickson, La Jolla, California). Samples for transparent exopolymer particles (TEP) were filtered onto 0.4 µm polycarbonate filters (Poretics) and subsequently stained with Alcian Blue following the procedure of Passow and Alldredge (1995). |
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Last Modified: 2024-05-31T18:50:46Z
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