Isotopes from B. gymnorhiza mangroves in Palau during 2013 (NCEI Accession 0278451)
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title: Isotopes from B. gymnorhiza mangroves in Palau during 2013 (NCEI Accession 0278451)
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abstract: This dataset contains physical data collected at shoreside Palau during deployment Ongael_2013-09 from 2013-09-12 to 2013-10-08. These data include salinity calculated from CTD primary sensors. The instruments used to collect these data include Elemental Analyzer and Isotope-ratio Mass Spectrometer. These data were collected by Dr Nemiah Ladd and Julian P. Sachs of University of Washington as part of the "Do Parallel Patterns Arise from Parallel Processes? (PaPaPro)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-05-26. The following is the text of the dataset description provided by BCO-DMO: Isotopes from B. gymnorhiza mangroves in Palau in 2013. Dataset Description: Lipid and water δ 2 H and δ 18 O from B. gymnorhiza mangroves in Palau during 2013.
purpose: This dataset is available to the public for a wide variety of uses including scientific research and analysis.
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Anchor: http://lod.bco-dmo.org/id/person/709236 Nemiah Ladd
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Anchor: http://lod.bco-dmo.org/id/person/51578 Sachs, Julian P.
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beginPosition: 2013-09-12
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supplementalInformation: Acquisition Description: All methodology was described in Ladd & Sachs, GCA, 2017, doi: 10.1016/j.gca.2017.01.046 Leaf and stem samples were collected from three replicate trees on the north shore of each lake and stored frozen prior to analysis. Water samples were collected from 1cm below the lake surface, and salinity was measured with a refractometer. Leaf and stem water were cryogenically extracted on a vacuum line at ETH Zurich. Leaf lipids were extracted using a Dionex Accelerated Solvent Extractor (ASE 200). Total lipid extracts were saponified with KOH in MeOH (12 hours at 60 deg C). Following saponification, hexane soluble lipids were then separated into hydrocarbon, wax ester, alcohol and polar fractions via a silica gel column chromatography. Alcohol fractions were acetylated with acetic anhydride of known H isotopic composition prior to analysis by GC.
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DateTime: 2023-05-19T04:32:20Z
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acquisitionInformation: (MI_AcquisitionInformation)
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code: mass spectrometer
type: mass spectrometer
description: Instrument that measure the massess and relative concentrations of atoms and molecules
