Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project) (NCEI Accession 0278234)
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title: Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project) (NCEI Accession 0278234)
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abstract: This dataset contains biological and survey - biological data collected on R/V Kilo Moana and R/V Thomas G. Thompson during cruises KM1301, KM1312, and TN277 in the North Pacific Ocean from 2012-03-01 to 2013-07-27. These data include depth, prochlorococcus abundance, and synechococcus abundance. The instruments used to collect these data include Flow Cytometer. These data were collected by Zackary I. Johnson of Duke University as part of the "Seasonal and decadal changes in temperature drive Prochlorococcus ecotype distribution patterns (POWOW)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-12-28. The following is the text of the dataset description provided by BCO-DMO: Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) by flow cytometry. Dataset Description: Phytoplankton concentrations from POWOW1 (TN277), POWOW2 (KM1301), and POWOW3 (KM1312) cruises including Prochlorococcus, Synechococcus and pico-photosynthetic eukaryotes (PEuks) determined by flow cytometry.
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title: Johnson, Z. I. (2014) Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 4) Version Date 2014-09-10. https://doi.org/10.26008/1912/bco-dmo.3901.4
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Anchor: http://lod.bco-dmo.org/id/person/50749 Zackary I. Johnson
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/298 Duke University
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Anchor: http://lod.bco-dmo.org/id/affiliation/191 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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supplementalInformation: Acquisition Description: Phytoplankton were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010). Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (<15°) scatter, inelastic side (90°) scatter (SSC), green (530 ± 30 nm) fluorescence, orange fluorescence (585 ± 42 nm), and red fluorescence (> 670 nm) emissions were measured. Populations were deemed ‘phytoplankton’ if they contained red-fluorescence, i.e. had a chlorophyll signal and were subsequently assigned to specific taxa (i.e. Prochlorococcus, Synechococcus or Pico-photosynthetic eukaryotes) based on previous classification schemes (Johnson et al., 2010). References: Johnson, Z.I., Shyam, R., Ritchie, A.E., Lin, Y., Mioni, C., Lance, V.P. et al. (2010) The effects of iron- and light-limitation on phytoplankton communities of deep chlorophyll maxima of the Western Pacific Ocean. Journal of Marine Research 68: 1-26. doi: 10.1357/002224010793721433
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fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
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code: Flow Cytometer
type: Flow Cytometer
description: A method to enumerate bacterial and phytoplankton cells in seawater and estimate their spherical diameter Flow cytometers are laboratory based instruments used to measure cells and particles from aqueous samples. The instrument was initially developed for analysis of Human blood cells for medical purposes, but this technology was adopted by the ocean science community by several investigators, principally Penny Chischolm out of the Massachusetts Institute of Technology, in late 20th century. Since then, the technique has been broadly applied, and it is a unique measurement of microbial populations in the ocean because it provides one at a time measures of cells, to extrapolate features such as fluorescence and cell size. Flow cytometers have been adapted for a number of oceanographic applications such as ship board measurement, and more precise measurement of planktonic fractions. Additionally, the flow cytometer has been adapted for in-situ deployment (see the corresponding nodc instrument code).
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code: R/V KILO MOANA
description: R/V; AGOR 26; Univ. of Hawaii; commissioned 2002; active July 2007
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description: Use this platform for data attributed to R/V Thomas G. Thompson that were collected between July 1991 and present. R/V Thomas G. Thompson (AGOR-23) Owned by US Navy, leased to University of Washington. Active dates: July 1991 (Source: http://www.navsource.org/archives/09/57/5723.htm, last accessed on 20 Dec 2011)
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