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title: Diel series of pico-cyanobacteria concentration and cell properties from the RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010 (NCEI Accession 0278153)
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abstract: This dataset contains biological and optical data collected on R/V Cape Hatteras during cruises CH0409 and CH0510 from 2009-05-26 to 2010-05-31. These data include depth and fluorescence. The instruments used to collect these data include Flow Cytometer and Niskin bottle. These data were collected by Dr Brian Binder of University of Georgia as part of the "Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach (Picophytoplankton_Regulation)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-06-11. The following is the text of the dataset description provided by BCO-DMO: Diel series of pico-cyanobacteria concentration and cell properties. Dataset Description: Diel series of pico-cyanobacteria concentration and cell properties. These data were published in Hynes et al., 2015 and Rhodes K.L., 2009 Other relevant publication: Binder et al., 1996
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1786 University of Georgia
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://lod.bco-dmo.org/id/person/50893
protocol: HTTPS
name: University of Georgia (UGA) website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) author
citedResponsibleParty: (CI_ResponsibleParty)
organisationName:
Anchor: https://ror.org/00vcb3m70 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: http://bcodmo.org
protocol: HTTP
name: Biological and Chemical Oceanography Data Management Office (BCO-DMO) website
description: Institution web page
function: (CI_OnLineFunctionCode) information
role: (CI_RoleCode) publisher
presentationForm: (CI_PresentationFormCode) tableDigital
associationType: (DS_AssociationTypeCode) crossReference
initiativeType: (DS_InitiativeTypeCode) collection
language:
LanguageCode: eng; USA
characterSet: (MD_CharacterSetCode) utf8
topicCategory: (MD_TopicCategoryCode) environment
topicCategory: (MD_TopicCategoryCode) oceans
extent: (EX_Extent)
geographicElement: (EX_GeographicBoundingBox)
westBoundLongitude: -72.8769
eastBoundLongitude: -71.8634
southBoundLatitude: 30.1464
northBoundLatitude: 30.9082
temporalElement: (EX_TemporalExtent)
extent:
TimePeriod:
beginPosition: 2009-05-26
endPosition: 2010-05-31
supplementalInformation: Acquisition Description: Samples were taken using a rosette of Niskin bottles, fixed with freshly titrated paraformaldehyde (pH 7.4–8.1, 0.1% final concentration), held in the dark for 10 min, frozen in liquid nitrogen, and stored in a -80 deg C freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until analysis. Preserved samples were analyzed by dual beam flow cytometry on a modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were chosen in random order, defrosted in a 30°C water bath (just long enough to melt, ~5 min), and stained with the DNA-specific stain Hoechst 33342 (0.5 ug mL-1 final concentration) (Invitrogen, Carlsbad, California) for a minimum of 20 min in the dark. Prior to analysis, polystyrene fluorescent beads (0.5 um and 1.0 um diameter Flow CheckVR ; Polysciences, Washington, Pennsylvania), were added to each sample, and used to normalize cellular light scatter and red (chlorophyll-derived) fluorescence. Samples were run at an infusion rate of 10 uL min-1 for 10–50 min, depending on cell abundance within the sample. Mean red fluorescence and forward angle light scatter for each cell type in each sample are linearized and normalized to 1.0 um and 0.5 um diameter beads (see above), respectively. Thus the measurements are relative, and are only meaningful for comparisons of cellular properties within this data set.
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distributionInfo: (MD_Distribution)
distributor: (MD_Distributor)
distributorContact: (CI_ResponsibleParty)
organisationName:
Anchor: https://ror.org/04r0wrp59 NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
phone: (CI_Telephone)
voice: +1-301-713-3277
address: (CI_Address)
electronicMailAddress: ncei.info@noaa.gov
role: (CI_RoleCode) pointOfContact
distributionOrderProcess: (MD_StandardOrderProcess)
fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
distributorFormat: (MD_Format)
name: TSV
version: (unknown)
distributorTransferOptions: (MD_DigitalTransferOptions)
transferSize:
Real: 0.18
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/0278153
protocol: HTTPS
applicationProfile: Web browser
name: NCEI Dataset Landing Page
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/oas/278153
protocol: HTTPS
applicationProfile: Web browser
name: Descriptive Information
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/download/278153
protocol: HTTPS
applicationProfile: Web browser
name: HTTPS
description: Navigate directly to the URL for data access and direct download.
function: (CI_OnLineFunctionCode) download
onLine: (CI_OnlineResource)
linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0212/0278153/
protocol: FTP
applicationProfile: Any FTP client
name: FTP
description: These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
function: (CI_OnLineFunctionCode) download
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dataQualityInfo: (DQ_DataQuality)
scope: (DQ_Scope)
level: (MD_ScopeCode) dataset
lineage: (LI_Lineage)
processStep: (LE_ProcessStep)
description: NCEI Accession 0278153 v1.1 was published.
dateTime:
DateTime: 2023-05-13T04:55:03Z
output: (LE_Source)
sourceCitation: (CI_Citation)
title: NCEI Accession 0278153 v1.1
date: (CI_Date)
date: (inapplicable)
dateType: (CI_DateTypeCode) publication
citedResponsibleParty: (CI_ResponsibleParty)
individualName: (inapplicable)
contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/0278153/1.1
protocol: HTTPS
name: NCEI Accession 0278153 v1.1
description: published 2023-05-13T04:55:03Z
function: (CI_OnLineFunctionCode) download
role: (inapplicable)
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metadataMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
maintenanceNote: Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) custodian
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acquisitionInformation: (MI_AcquisitionInformation)
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: bottle
type: bottle
description: any type of water bottle sampling device Generic name for water collection device; usually used to determine temperature, salinity and provide water aliquots for measurement of a wide range of parameters; often referred to by a specific type of water sampling bottle, such as a Nansen or Niskin bottle.
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: Flow Cytometer
type: Flow Cytometer
description: A method to enumerate bacterial and phytoplankton cells in seawater and estimate their spherical diameter Flow cytometers are laboratory based instruments used to measure cells and particles from aqueous samples. The instrument was initially developed for analysis of Human blood cells for medical purposes, but this technology was adopted by the ocean science community by several investigators, principally Penny Chischolm out of the Massachusetts Institute of Technology, in late 20th century. Since then, the technique has been broadly applied, and it is a unique measurement of microbial populations in the ocean because it provides one at a time measures of cells, to extrapolate features such as fluorescence and cell size. Flow cytometers have been adapted for a number of oceanographic applications such as ship board measurement, and more precise measurement of planktonic fractions. Additionally, the flow cytometer has been adapted for in-situ deployment (see the corresponding nodc instrument code).
platform: (MI_Platform)
identifier: (MD_Identifier)
code: CAPE HATTERAS
description: CAPE HATTERAS
instrument: (missing)
