Estimated abundances of viruses and bacteria determined in samples collected in the Eastern Tropical North Pacific (ETNP) on R/V New Horizon cruise NH1315 during June 2013 (NCEI Accession 0278126)
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title: Estimated abundances of viruses and bacteria determined in samples collected in the Eastern Tropical North Pacific (ETNP) on R/V New Horizon cruise NH1315 during June 2013 (NCEI Accession 0278126)
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abstract: This dataset contains biological and survey - biological data collected on R/V New Horizon during cruise NH1315 in the North Pacific Ocean from 2013-06-19 to 2013-06-22. These data include abundance and depth. The instruments used to collect these data include Fluorescence Microscope and Niskin bottle. These data were collected by Jennifer R. Brum of University of Arizona as part of the "Ecology and biogeochemical impacts of viruses in marine oxygen minimum zones (OMZ Viruses)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-09-16. The following is the text of the dataset description provided by BCO-DMO: Estimated abundances of viruses and bacteria via epifluorescence microscopy in the ETNP Dataset Description: Estimated abundances of viruses and bacteria via epifluorescence microscopy of samples collected in the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) on R/V New Horizon cruise NH1315 from 13-28 June 2013.
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1395 University of Arizona
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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beginPosition: 2013-06-19
endPosition: 2013-06-22
supplementalInformation: Acquisition Description: Detailed protocols, including suggestions from the scientific community, are published on the lab website at https://u.osu.edu/viruslab/protocols/ and maintained on protocols.io at https://www.protocols.io/workspaces/sullivan-lab . Samples were collected from the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) during the OMZ Microbial Biogeochemistry Expedition cruise (R/V NewHorizon,13-28 June 2013). Seawater was collected from 16 depths spanning the mixed layer, oxycline, OMZ core, and below the OMZ. Collections were made using Niskin bottles on a rosette. Samples were preserved with EM-grade glutaraldehyde (2% final concentration), flash-frozen in liquid nitrogen and stored between -72 °C and -80 °C until analysis. Viral and bacterial concentrations were determined based on a previously described method (Noble and Fuhrman, 1998) in which thawed samples were filtered onto 0.02-μm- pore-size filters (Anodisc, Whatman, GE Healthcare Life Sciences, Piscataway, NJ, USA), stained with SYBR Gold nucleic acid stain (Invitrogen, Life Technologies, Carlsbad, CA, USA) and enumerated using an epifluorescence microscope (Axio Imager. D2, Zeiss, Jena, Germany).
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
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fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
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linkage: https://www.ncei.noaa.gov/archive/accession/oas/278126
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linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0212/0278126/
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description: NCEI Accession 0278126 v1.1 was published.
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DateTime: 2023-05-13T04:14:30Z
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acquisitionInformation: (MI_AcquisitionInformation)
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identifier: (MD_Identifier)
code: bottle
type: bottle
description: any type of water bottle sampling device Generic name for water collection device; usually used to determine temperature, salinity and provide water aliquots for measurement of a wide range of parameters; often referred to by a specific type of water sampling bottle, such as a Nansen or Niskin bottle.
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code: fluorescence microscope
type: fluorescence microscope
description: Light Stimulating Microscope An optical microscope used to study properties of substances using fluorescence. The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed, causing light of longer wavelengths to be emitted.
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code: NEW HORIZON
description: Flag: United States Type: Research Vessel IMO: 7723821 Operator: Scripps DOB: 1978-09
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