Water column data sampled aboard the R/V Pelican during August and September 2016 and May 2017 in Northern Gulf of Mexico, specifically the Louisiana Shelf region dominated by the discharge of the Mississippi River plume (NCEI Accession 0278026)
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title: Water column data sampled aboard the R/V Pelican during August and September 2016 and May 2017 in Northern Gulf of Mexico, specifically the Louisiana Shelf region dominated by the discharge of the Mississippi River plume (NCEI Accession 0278026)
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abstract: This dataset contains biological, chemical, optical, physical, and survey - biological data collected on R/V Pelican during cruises PE17-04 and PE17-20 in the Gulf of Mexico from 2016-08-28 to 2017-05-12. These data include Ammonium, Nitrite, PAR, Silicate, Total Dissolved Nitrogen, beam attenuation, chlorophyll a, depth, depth_bottom, diatom abundance, dissolved Oxygen, fluorescence, nitrate plus nitrite, reactive phosphorus (PO4), salinity calculated from CTD primary sensors, total phaeopigment, and water temperature. The instruments used to collect these data include Fluorometer, GM multicounter, and Nutrient Autoanalyzer. These data were collected by Jeffrey W. Krause of Dauphin Island Sea Lab and Kanchan Maiti of Louisiana State University as part of the "The biotic and abiotic controls on the Silicon cycle in the northern Gulf of Mexico (CLASiC)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-09-03. The following is the text of the dataset description provided by BCO-DMO: Acquisition Description: Hydrocasts were conducted at identified stations. A SeaBird CTD and rosette system, owned and maintained by Louisiana Universities Marine Consortium (LUMCON), operating institution for the R/V Pelican, was used for sampling. Calibration information can be found associated with the CTD data. Unless otherwise stated, samples used for rate measurements were collected based on the percent irradiance relative to that just below the surface. Water was sampled from Niskin bottles and pooled into 10 L acid-cleaned carboys. For inorganic nutrients, water was filtered using 0.6 μm pore size polycarbonate membrane and immediately frozen until analysis. Filtered water was analyzed for total dissolved nitrogen (TDN), nitrate+nitrite (NO 3 + NO 2 ), nitrite, soluble reactive phosphate (SRP), and ammonium (NH 4 ) colorimetrically using Skalar autoanalyzer (Dzwonkowski et al. 2017), and for dissolved silicic acid (Si(OH) 4 ) using a manual colorimetric method (Krause et al. 2009). Water for Chlorophyll a was filtered through a 0.45 μm-pore 47mm diameter HAWP Millipore filter, immediately frozen, and analyzed on shore (<2 weeks) using an acetone extraction/acidification method (Lomas et al. 2019). For biogenic silica analysis, seawater was filtered through a 1.2 μm-pore polycarbonate filter (47 mm diameter) and frozen immediately. On shore, filters were dried and analyzed using a sodium-carbonate time course digestion, to correct for lithogenic silica interference, in polymethylpentene tubes (Pickering et al. in review). In 2016, NaOH digestions were also done for biogenic silica, followed by an HF digestion to quantify lithogenic silica as in Krause et al. (2009). Diatom abundance was quantified by fixing samples with Bouin's solution; in the laboratory, cells were settled in a chamber and enumerated (Utermöhl 1958). Diatom rate processes were quantified using a radioisotope ( 32 Si) and fluorescent dye (PDMPO) tracers. Sample bottles were incubated for 12 or 24 hours in acrylic incubators cooled with continually flowing surface water under a series of neutral density screens to simulate light levels at the depth of collection (i.e. see above). Measurement for ambient (Amb) conditions (i.e. no enrichment of Si(OH) 4 ) and enhanced (Enh) conditions (i.e. +20 µM enrichment of Si(OH) 4 ) were made at most stations and depths using 32 Si. The gross rate of biogenic silica production was measured using the radioisotope tracer 32 Si with high specific activity (>40 kBq µg Si -1 ) as described in Krause et al. (2011). For PDMPO uptake, dye was added to samples at ambient or enriched Si(OH) 4 , incubated in the same conditions as the 32 Si samples, and processed as in McNair et al. (2015). The net rate of biogenic silica production was calculated from biogenic silica standing stock at the time of sampling and after a 24-hour incubation, under the same conditions as the 32 Si and PDMPO, as described in Krause et al. (2010).
purpose: This dataset is available to the public for a wide variety of uses including scientific research and analysis.
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Anchor: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1558957 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1558957 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1558957
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