32Si and 14C production data (experimental) from EXPORTS cruise RR1813 on R/V Roger Revelle in the Subarctic North Pacific near Station PAPA from August to September 2018 (NCEI Accession 0277945)
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title: 32Si and 14C production data (experimental) from EXPORTS cruise RR1813 on R/V Roger Revelle in the Subarctic North Pacific near Station PAPA from August to September 2018 (NCEI Accession 0277945)
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abstract: This dataset contains biological, chemical, and physical data collected on R/V Roger Revelle during cruise RR1813 in the North Pacific Ocean from 2018-08-16 to 2018-09-07. These data include Nitrite, PAR, SiOH_4, depth, nitrate plus nitrite, particulate organic Carbon (POC), particulate organic nitrogen, primary production, and reactive phosphorus (PO4). The instruments used to collect these data include CTD Sea-Bird 9, Flow Injection Analyzer, GO-FLO Bottle, and Light-Dark Bottle. These data were collected by Mark A. Brzezinski of University of California-Santa Barbara, Bethany D. Jenkins of University of Rhode Island, and Kristen N. Buck of University of South Florida as part of the "Collaborative Research: Diatoms, Food Webs and Carbon Export - Leveraging NASA EXPORTS to Test the Role of Diatom Physiology in the Biological Carbon Pump (Diatoms and carbon export)" project and "EXport Processes in the Ocean from Remote Sensing (EXPORTS)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-02-06. The following is the text of the dataset description provided by BCO-DMO: 32Si and 14C production data (experimental) Dataset Description: Depth profiles in the euphotic zone of nutrient (nitrate, silicate, phosphate) concentrations, profiles of silicic acid uptake rates and assessment of limitation by Si and Fe on both silicic acid uptake and carbon fixation. See related dataset: https://www.bco-dmo.org/dataset/785856
purpose: This dataset is available to the public for a wide variety of uses including scientific research and analysis.
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supplementalInformation: Acquisition Description: Seawater samples were collected using an epoxy coated CTD-rosette mounted with Go-Flo samplers and a Sea-Bird Electronics CTD (SBE9plus). Go-Flo bottles were transferred to a trace metal clean van for subsampling into polypropylene tubes (nutrients), polypropylene bottle (biogenic silica and particulate carbon and nitrogen) or TM acid-cleaned polycarbonate incubation bottles (Si-32 & C-14 incubation experiments). Nutrient samples were filtered through 0.2 μm polycarbonate filters and frozen at -20°C. Samples for biogenic silica concentrations were size fractionated by serial filtration through 5 μm and 0.6 μm polycarbonate filters. Filters were stored frozen at -20°C. Particulate organic carbon and nitrogen were measured on samples from experiments examining the effect of added Fe and Si on carbon fixation. These samples were filtered through precombusted GFF filters placed in glass scintillation vials and frozen at -20°C. Samples for silicic acid uptake profiles were spiked with the radioisotope Si-32. Nutrient limitation assays were performed on pairs of samples where rate of silicic acid uptake (Si-32) or carbon fixation (C-14 in paired light/dark bottles) were determined in unaltered controlled samples and in samples augmented with either silicic acid (20 μM) or iron chloride (1 nM). All samples were incubated on deck in simulated in situ incubators cooled with flowing surface seawater from 24 h. Profiles samples six depths from near surface to the 1% light level. Nutrient limitation assays were performed at the 40% and 10% light levels. Particles from incubated samples were size fractionated by serial filtration through 5 μm and 0.6 μm 25 mm polycarbonate filters. For C-14 incubations, total radioactivity in each sample was determined by sampling 100 μl of sample seawater prior to filtration. Filters from Si-32 incubations were placed on plastic planchettes and dried before covering with mylar film and stored or analysis ashore using low level beta counters (Riso Inc). Filters from C-14 incubations were acidified in glass scintillation vials, scintillation cocktail (Ultima Gold XR) added followed by liquid scintillation counting. Total radioactivity samples received 100 μL of b-phenethylamine and 5 mL of scintillation cocktail prior to analysis at sea using a Beckman 8500 scintillation counter. For more information, see the Protocol documents (under Supplemental Files).
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