Metal quotas of NE Pacific and Atlantic diatom isolates measured via ICP-MS after growth in zinc and cobalt media amendments in experiments with cultures collected from R/V Thomas G. Thompson cruise TN280, along Line P in the NE Pacific, in May of 2012 (NCEI Accession 0277718)
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title: Metal quotas of NE Pacific and Atlantic diatom isolates measured via ICP-MS after growth in zinc and cobalt media amendments in experiments with cultures collected from R/V Thomas G. Thompson cruise TN280, along Line P in the NE Pacific, in May of 2012 (NCEI Accession 0277718)
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abstract: This dataset contains chemical and physical data collected on R/V Thomas G. Thompson during cruise TN280 from 2012-05-17 to 2012-05-22. These data include trace metal concentration. The instruments used to collect these data include Mass Spectrometer. These data were collected by Mak A. Saito of Woods Hole Oceanographic Institution as part of the "Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)" and "US GEOTRACES PMT: Cobalt Biogeochemical Cycling and Connections to Metalloenzymes in the Pacific Ocean (PMT Cobalt and Metalloenzymes)" projects. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-04-10. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Metal quotas of two NE Pacific Line P diatom isolates and one Atlantic diatom isolate measured via ICP-MS after growth in zinc and cobalt media amendments. Experiments with cultures collected from the GeoMICS expedition on the R/V Thomas G. Thompson (cruise TN280), along Line P in the NE Pacific, in May of 2012.
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individualName:
Anchor: http://lod.bco-dmo.org/id/person/50985 Saito, Mak A.
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/742 Woods Hole Oceanographic Institution
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name: Woods Hole Oceanographic Institution website
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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name: Biological and Chemical Oceanography Data Management Office website
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language:
LanguageCode: eng; USA
characterSet: (MD_CharacterSetCode) utf8
topicCategory: (MD_TopicCategoryCode) environment
topicCategory: (MD_TopicCategoryCode) oceans
extent: (EX_Extent)
geographicElement: (EX_GeographicBoundingBox)
westBoundLongitude: -128.666
eastBoundLongitude: -4
southBoundLatitude: 40.756
northBoundLatitude: 54
temporalElement: (EX_TemporalExtent)
extent:
TimePeriod:
beginPosition: 2012-05-17
endPosition: 2012-05-22
supplementalInformation: Acquisition Description: Location: Cultures collected from Northeast Pacific Line P Transect 48.8167 N 128.667 W Metal quotas Cellular metal quotas were measured by inductively coupled plasma mass spectrometry (ICP-MS). Biomass from replicate 25 mL matrix cultures of P. tricornutum CCMP632, P. delicatissima UNC1205, and Thalassiosira UNC1203 were pooled upon entering stationary phase and were centrifuged at 11,000 RPM (14,610 x g) for 40 minutes at 4°C. The cell pellet was resuspended in ~1 mL media and transferred to an acid-cleaned microcentrifuge tube. Cultures were centrifuged again for 30 min at 14,100 RPM (13,336 x g) at 4°C before the supernatant was discarded. The remaining cell pellet was acidified in 800 L of 5% nitric acid (Optima) containing 1 ppb indium for at least seven days. Solids were removed by centrifugation. No attempt was made to remove extracellular metals by washing cells with additional metal chelators in order to minimize processing blanks. Quota determinations therefore include contributions from both intracellular and extracellular pools. Process blank digestions containing acid but no cells were performed in parallel. Digests were diluted by a factor of 9 with 5% nitric acid 1 ppb indium solution before being analyzed in duplicate on a Thermo ICAP-Q plasma mass spectrometer calibrated to a multi-element standard curve (Spex Certiprep) over a range of 1 – 20 ppb. Samples were analyzed in KED mode after an 85s sample uptake window and element mass windows were scanned 3 times during measurements. The 1 ppb indium internal standard was used to correct for variation in sample delivery and plasma suppression between samples. Process blanks were subtracted from measured concentrations. Phosphorus concentrations were also measured by ICP-MS simultaneously and were calibrated to a standard curve ranging from 100 – 3,200 ppb using a 1 ppm certified P stock (Alfa Aesar Specpure). The seawater media base used for all growth experiments was similarly analyzed via ICP-MS using a 1:10 dilution of media base into 5% nitric acid 1 ppb indium and analyzed as above to determine background media concentrations of total Zn and Co (0.9 nmol L-1 and 0.1 nmol L-1, respectively). Missing data identifiers in this dataset include: * "nd" indicating no data * Below Detection * "contam" indicating the sample was contaminated Isolation sources and locations * Pseudonitzschia delicatissima UNC1205 and Thalassiosira UNC1203 were isolated from station P8 of the Line P transect, 48.817N 128.666W * Phaeodactylum tricornutum CCMP632 was ordered from Bigelow, the strains original location of isolation was 54N 4W * Thalassiosira pseudonana CCMP1335 was also from Bigelow, original location of isolation was 40.756° N 72.82° W
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
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voice: +1-301-713-3277
address: (CI_Address)
electronicMailAddress: NCEI.Info@noaa.gov
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fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
distributorFormat: (MD_Format)
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linkage: https://www.ncei.noaa.gov/archive/accession/0277718
protocol: HTTPS
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name: NCEI Dataset Landing Page
description: Navigate directly to the URL for a descriptive web page with download links.
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linkage: https://www.ncei.noaa.gov/archive/accession/oas/277718
protocol: HTTPS
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description: Navigate directly to the URL for a descriptive web page with download links.
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onLine: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/archive/accession/download/277718
protocol: HTTPS
applicationProfile: Web browser
name: HTTPS
description: Navigate directly to the URL for data access and direct download.
function: (CI_OnLineFunctionCode) download
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linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0212/0277718/
protocol: FTP
applicationProfile: Any FTP client
name: FTP
description: These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
function: (CI_OnLineFunctionCode) download
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dataQualityInfo: (DQ_DataQuality)
scope: (DQ_Scope)
level: (MD_ScopeCode) dataset
lineage: (LI_Lineage)
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description: NCEI Accession 0277718 v1.1 was published.
dateTime:
DateTime: 2023-04-20T04:38:33Z
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title: NCEI Accession 0277718 v1.1
date: (CI_Date)
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name: NCEI Accession 0277718 v1.1
description: published 2023-04-20T04:38:33Z
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role: (inapplicable)
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metadataMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
maintenanceNote: Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) custodian
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acquisitionInformation: (MI_AcquisitionInformation)
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: mass spectrometer
type: mass spectrometer
description: Instrument that measure the massess and relative concentrations of atoms and molecules
platform: (MI_Platform)
identifier: (MD_Identifier)
code: R/V THOMAS G. THOMPSON
description: Use this platform for data attributed to R/V Thomas G. Thompson that were collected between July 1991 and present. R/V Thomas G. Thompson (AGOR-23) Owned by US Navy, leased to University of Washington. Active dates: July 1991 (Source: http://www.navsource.org/archives/09/57/5723.htm, last accessed on 20 Dec 2011)
instrument: (missing)
