Protist rates from epifluorescence counts; cyanobacteria rates from flow cytometry; bulk rates from extracted chlorophyll-a from R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea, BATS, 2011-2012 (NCEI Accession 0277441)
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title: Protist rates from epifluorescence counts; cyanobacteria rates from flow cytometry; bulk rates from extracted chlorophyll-a from R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea, BATS, 2011-2012 (NCEI Accession 0277441)
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abstract: This dataset contains biological data collected on R/V Atlantic Explorer during cruises AE1102, AE1118, AE1206, and AE1219 in the North Atlantic Ocean from 2011-02-25 to 2012-07-30. These data include depth, growth, and taxon. The instruments used to collect these data include Niskin bottle and Turner Designs 700 Laboratory Fluorometer. These data were collected by Susanne Neuer of Arizona State University as part of the "Plankton Community Composition and Trophic Interactions as Modifiers of Carbon Export in the Sargasso Sea (Trophic BATS)" project and "Ocean Carbon and Biogeochemistry (OCB)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23. The following is the text of the dataset description provided by BCO-DMO: Protist rates based on epifluorescence counts; cyanobacteria rates based on flow cytometry data (Lomas group); bulk rates based on extracted chlorophyll-a. Dataset Description: Protist rates based on epifluorescence counts; cyanobacteria rates based on flow cytometry data (Mike Lomas' group); bulk rates based on extracted chlorophyll-a. Samples were collected during four cruises in the Sargasso Sea during spring and summer 2011-2012.
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Anchor: http://lod.bco-dmo.org/id/person/51336 Susanne Neuer
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1878 Arizona State University (ASU)
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Anchor: http://lod.bco-dmo.org/id/affiliation/191 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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Anchor: http://lod.bco-dmo.org/id/person/51336 Neuer, Susanne
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1416 Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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LanguageCode: eng; USA
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topicCategory: (MD_TopicCategoryCode) oceans
topicCategory: (MD_TopicCategoryCode) biota
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beginPosition: 2011-02-25
endPosition: 2012-07-30
supplementalInformation: Acquisition Description: Water Column Sampling: Water column sampling was performed on four cruises during the spring and the summer of 2011 and 2012 at the Bermuda Atlantic Time-series Study station (31’40°N 64’10°W, BATS) and in the mesoscale eddies found in the surrounding area of the Sargasso Sea. For each cruise, two stations were sampled, usually in the center of a mesoscale eddy and at BATS. The edge of the eddy was sampled two times, as well. To be able to get a better reproducibility of data, each experiment was replicated. For each experiment, seawater samples were collected pre-dawn (on deck 2:30-4:00, local time) at four different depths within the euphotic zone (20m, 50m, 80m and the Deep Chlorophyll Maximum, DCM). Twenty-one 10L Niskin bottles were attached to a rosette with conductivity, temperature, depth sensors (CTD), and an in vivo fluorometer. This sensor allowed for recording in real time of chlorophyll fluorescence and the DCM for each station. The water that was collected from the 10L Niskin bottles was sampled for abundance and biomass of the plankton community. Dilution experiments: The growth and grazing rates of the phytoplankton community were determined using the dilution method with a two-point modification (Landry et al. 1984, Landry et al. 2008a, Selph et al. 2011). To check the applicability and resolution of the two point modification, two experiments at two different stations (one replicate of C3 and one replicate of B3) were carried out with a complete set of dilutions (25, 50, 75 and 100%) on-deck; the results of these experiments confirmed the validity of the 2 point dilution experiment (data not shown). The Landry-Hassett dilution method was used because it can separate autotrophic and heterotrophic processes with relatively little manipulation (Landry & Hassett, 1982, Landry et al 1984). The method is based on the concept that the dilution lowers the encounter probability of grazer and prey and enables one to calculate from the net phytoplankton population growth (k) in the diluted and undiluted incubation bottles the instantaneous growth rate (u) and grazing mortality (g), where k= u - g (Landry & Hassett, 1982). This method is based on three assumptions. The first one is that the instantaneous growth rate of any phytoplankton group is not influenced by the dilution of the “seawater/sample” with particle free water. The second assumption is that the probability of a phytoplankton to be grazed by the consumer is directly related to the abundance of the consumer itself, which means that with a higher density of grazers the probability of the prey to be grazed is higher as well, making the grazing a function of the dilution. The third assumption is the sum of the first two assumptions, that the change in the abundance of the phytoplankton community over time can be expressed by the exponential growth equation: P t =P 0 e ^ [ (u -xg)t ] where P 0 and P t are the initial and the final abundances or biomass of bulk or specific phytoplankton groups, t is time in day (d), u is the instantaneous phytoplankton growth rate (d -1 ), g is the grazing mortality (d -1 ), and x represents the dilution factor (Landry and Hassett 1982; Landry et al. 1984; Neuer and Cowles 1994). M and g are calculated as the y-intercept and the slope of the linear regression plotted against the dilution factors where k=1/t ln (P t /P 0 ) (Landry & Hassett, 1982; Landry et al. 1984). For the two-point dilution, this converts mathematically to solving the equations for u and g (Landry et al., 2008a). Bulk measurements : Chlorophyll-a was extracted from seawater (250 ml and 400 ml depending on the dilution), with 90% acetone and measured after 24hrs at 4 degrees C in the dark onboard the ship using a TD 700 Laboratory Fluorometer using the non-acidification technique (Welschmeyer 1994). These data were used as a proxy for the phytoplankton biomass in the water column and to calculate the bulk growth and grazing rates of the phytoplankton community.
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
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fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
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linkage: https://www.ncei.noaa.gov/archive/accession/0277441
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linkage: https://www.ncei.noaa.gov/archive/accession/oas/277441
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linkage: https://www.ncei.noaa.gov/archive/accession/download/277441
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linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0211/0277441/
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description: NCEI Accession 0277441 v1.1 was published.
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DateTime: 2023-04-03T13:05:10Z
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acquisitionInformation: (MI_AcquisitionInformation)
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code: bottle
type: bottle
description: any type of water bottle sampling device Generic name for water collection device; usually used to determine temperature, salinity and provide water aliquots for measurement of a wide range of parameters; often referred to by a specific type of water sampling bottle, such as a Nansen or Niskin bottle.
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code: RV Atlantic Explorer
description: Updated information from ICES for for the vessel Atlantic Explorer from UNITED STATES. Code : 33H4 Name : Atlantic Explorer Flag : UNITED STATES - US (ISO Country Code) Call Sign : WDC9417 Title : RV Platform Class : 31 Research vessel IMO : 8120014 Commissioned Date : 2005-10 Current Length : 51 Built : 1982 Previous Name (Notes): Seward Johnson II Notes : Bermuda Biological Station, Bermuda Institute of Ocean Sciences (BIOS) since 2005-10. Built as Trojan then renamed Edwin Link and Seward Johnson II. Active as at 2007-07. Lloyds URL : http://www.lloydsmiu.com/lmiu/vessels/overview.htm?vesselID=303109 Additional Information Sources: - http://www.marinetraffic.com/ais/shipdetails.aspx?MMSI=303591000 Same Vessels Info: 33EL - EDWIN LINK 33S2 - SEWARD JOHNSON II General Comments: Call sign: WDC9417 - information from ship captain. Clarified faulty info from the following: Oceanic=WST4798, Lloyds = WDC8916 , ITU = WDC9417
instrument: (missing)
