Phytoplankton clone library matches collected from the R/V Atlantic Explorer and R/V Oceanus cruises along the Bermuda Atlantic Time Series Station (BATS) from 2008-2010 (Plankton particle flux project) (NCEI Accession 0277334)
This dataset contains biological data collected on R/V Atlantic Explorer and R/V Oceanus during cruises AE0901, AE0902, AE1001, AE1006, AE1009, and OC449-10 from 2008-12-14 to 2010-04-24. These data include depth and taxon. The instruments used to collect these data include CTD profiler, Niskin bottle, and Sediment Trap. These data were collected by Susanne Neuer of Arizona State University as part of the "Composition of the plankton community and its contribution to particle flux in the Sargasso Sea (Plankton particle flux)" project and "Ocean Carbon and Biogeochemistry (OCB)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.
The following is the text of the dataset description provided by BCO-DMO:
Phytoplankton clone library matches from BATS cruises 2008-2010
Dataset Description:
This dataset includes links to GenBank accession of 18S rRNA gene clone library sequences with the closest sequence matches from the NCBI database. The samples are from the upper water column as well as from shallow drifting traps during regular BATS cruises from December 2008-April 2010.
The following is the text of the dataset description provided by BCO-DMO:
Phytoplankton clone library matches from BATS cruises 2008-2010
Dataset Description:
This dataset includes links to GenBank accession of 18S rRNA gene clone library sequences with the closest sequence matches from the NCBI database. The samples are from the upper water column as well as from shallow drifting traps during regular BATS cruises from December 2008-April 2010.
Dataset Citation
- Cite as: Neuer, Susanne (2023). Phytoplankton clone library matches collected from the R/V Atlantic Explorer and R/V Oceanus cruises along the Bermuda Atlantic Time Series Station (BATS) from 2008-2010 (Plankton particle flux project) (NCEI Accession 0277334). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277334. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0277334
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2008-12-14 to 2010-04-24 |
Spatial Bounding Box Coordinates |
West: -64.6504
East: -63.7119
South: 31.1715
North: 32.4006
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Water column samples were collected at BATS during the 2009 and 2010 winter bloom periods from the upper 150 m in Niskin bottles attached to a sampling rosette. Particle interceptor traps (PITs) with both fixed (2% formalin final concentration, deployment time 72 hr) and unfixed (deployment time 24 hr) collection tubes were deployed at 150 m depth in close proximity to the station and during the same time period as the water column collection in order to collect sinking particles below the euphotic zone. Clone libraries were constructed from 2-L samples collected at two depths in the euphotic zone, one close to the surface (10 m) and one from either the bottom of the euphotic zone if the water column was well mixed (most stations sampled; around 120-130 m) or from the chlorophyll maximum if one was present (around 80 m; samples from March 24 and April 24, 2010). Libraries were also constructed from sinking particles collected in two tubes of the PITs from the trap array located at 150 m depth. Unfixed traps were selected unless the sample was unavailable or prior results using DGGE indicated that the fixed trap would be more suitable due to occurrence of fungi and metazoans in the unfixed traps. DNA was extracted from these samples and a region of the 18S ribosomal RNA gene was amplified by PCR using eukaryotic primers. Samples from selected dates and depths were cloned and sequenced and broad taxonomic groups were determined for each sequence using their closest similarity match from BLAST (Basic Local Alignment Search Tool, http://blast.ncbi.nlm.nih.gov/ ). |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Platform keywords | NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes |
Instrument keywords | NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names |
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Project keywords | BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Cruise IDs Provider Funding Award Information |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov