Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy, from samples collected on R/V Knorr cruise KN210-04 in the Western Atlantic Ocean in 2013 (Deep Atlantic DOM project) (NCEI Accession 0277273)
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title: Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy, from samples collected on R/V Knorr cruise KN210-04 in the Western Atlantic Ocean in 2013 (Deep Atlantic DOM project) (NCEI Accession 0277273)
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abstract: This dataset contains biological, physical, and survey - biological data collected on R/V Knorr during cruise KN210-04 in the North Atlantic Ocean and South Atlantic Ocean from 2013-03-26 to 2013-05-06. These data include abundance, bacterial abundance, depth, and water pressure. The instruments used to collect these data include Fluorescence Microscope. These data were collected by Ian Hewson of Cornell University as part of the "Dissolved Organic Matter Composition in the Deep Atlantic Ocean (Deep Atlantic DOM)" project and "Ocean Carbon and Biogeochemistry (OCB)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23. The following is the text of the dataset description provided by BCO-DMO: Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy. Dataset Description: Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy. Duplicates were collected at each depth during the KN210-04 cruise.
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title: Hewson, I. (2014) Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy, from samples collected on R/V Knorr cruise KN210-04 in the Western Atlantic Ocean in 2013 (Deep Atlantic DOM project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-08-08. https://doi.org/10.26008/1912/bco-dmo.528510.1
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Anchor: http://lod.bco-dmo.org/id/person/51669 Ian Hewson
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LanguageCode: eng; USA
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supplementalInformation: Acquisition Description: Samples for viral and bacterial counts were collected in 50 mL Falcon tubes, held at 4 degrees C until processing, and processed within two hours. SYBR slides were prepared according to Noble and Fuhruman (Noble and Fuhrman, 1998). Briefly, samples were fixed in v/v 2% formalin and then filtered over a 25 mm 0.02 um anodisc filter (Whatman). Varying volumes were fixed depending on the depth of sample collection (surface and DCM: 3 ml; mesopelagic: 10 ml; AAIW, NADW and AABW: 20 ml). The filter was stained with 2.5x10-3 SYBR Green 1 dilution in nuclease free water, and mounted on a slide with antifade solution (0.1% p-phenylenediamine in 1:1 PBS:Glycerol) and stored at -20 degrees C until enumeration. Under epifluorescence microscopy 10 fields of view were selected at random and >200 viruses and >200 bacteria were counted. Calculations for total number or viruses and bacteria in a sample account for volume filtered, magnification and size of the grid included while counting. Samples from station 1 and 2 were fixed with the wrong concentration of antifade and therefore faded before accurate counts could be made. References: Noble, R. T., and Fuhrman, J. A. (1998). Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria. Aquat Microb Ecol 14 , 113-118. doi: 10.3354/ame014113
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DateTime: 2023-03-30T15:25:31Z
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acquisitionInformation: (MI_AcquisitionInformation)
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code: fluorescence microscope
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description: Light Stimulating Microscope An optical microscope used to study properties of substances using fluorescence. The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed, causing light of longer wavelengths to be emitted.
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code: R/V Knorr
description: Title: R/V Flag: United States of America Vessel Type: Research Vessel (Oceanographic) DOB: 1970 IMO: 7738618 MMSI: 367246000
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