Skip to main content
Dataset Overview | National Centers for Environmental Information (NCEI)

Cellular abundances of bacterioplankton and eukaryotic picoplankton measured by flow cytometry in water samples collected on NOAA Ship Ronald H. Brown during the West Coast Ocean Acidification Cruise led by the Pacific Marine Environmental Laboratory (PMEL) in the northern California current ecosystem from 2016-05-24 to 2016-06-16 (NCEI Accession 0265154)

browse graphicPreview graphic
Changes in ocean conditions such as chemical and thermal shifts are habitat pressures on marine organisms. As the basis of the marine food web, microorganisms such as bacteria and phytoplankton are responsible for important biogeochemical processes, such as nutrient cycling, and the primary production. They are also part of the environment surrounding aquatic organisms such as marine mammals, and serve as a source of both beneficial and harmful microbes associated with larger organisms. Due to their small size and direct physiological interactions with seawater, these microorganisms can rapidly respond to environmental changes, resulting in shifts in community composition and in relative abundances of community members. Shifts at these trophic levels can cause a ripple effect in the structure and function of the ecosystem for coastal and offshore species. Most assessments of biological responses to ocean acidification (OA) have been performed by controlled conditions, and there have been few opportunities to evaluate real-world relationships between biology and OA. This project leverages the sampling and chemical analyses conducted during the northern leg of the 2016 West Coast Ocean Acidification Cruise led by the Pacific Marine Environmental Laboratory (PMEL) to document ocean chemistry in the California Current during a period of likely coastal upwelling and greater ocean acidification. During that cruise, water from the same water samples that were used for chemical measurements by PMEL were preserved & analyzed for cellular abundances of bacterioplankton and eukaryotic picoplankton.
  • Cite as: Rhodes, Linda D.; Adams, Nicolaus G.; Alin, Simone R.; Feely, Richard A. (2022). Cellular abundances of bacterioplankton and eukaryotic picoplankton measured by flow cytometry in water samples collected on NOAA Ship Ronald H. Brown during the West Coast Ocean Acidification Cruise led by the Pacific Marine Environmental Laboratory (PMEL) in the northern California current ecosystem from 2016-05-24 to 2016-06-16 (NCEI Accession 0265154). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0265154. Accessed [date].
gov.noaa.nodc:0265154
Download Data
  • HTTPS (download)
    Navigate directly to the URL for data access and direct download.
  • FTP (download)
    These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
Distribution Formats
  • Excel
Ordering Instructions Contact NCEI for other distribution options and instructions.
Distributor NOAA National Centers for Environmental Information(link is external)
+1-301-713-3277
ncei.info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information(link is external)
ncei.info@noaa.gov
Time Period 2016-05-24 to 2016-06-16
Spatial Bounding Box Coordinates
West: -129.4498
East: -123.9122
South: 40.2463
North: 52.3992
Spatial Coverage Map
General Documentation
Associated Resources
Publication Dates
  • publication: 2022-10-18
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Purpose Determine relationship(s) of microbial abundances to ocean chemistry conditions
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Rhodes, Linda D.; Adams, Nicolaus G.; Alin, Simone R.; Feely, Richard A. (2022). Cellular abundances of bacterioplankton and eukaryotic picoplankton measured by flow cytometry in water samples collected on NOAA Ship Ronald H. Brown during the West Coast Ocean Acidification Cruise led by the Pacific Marine Environmental Laboratory (PMEL) in the northern California current ecosystem from 2016-05-24 to 2016-06-16 (NCEI Accession 0265154). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0265154. Accessed [date].
Cited Authors
Principal Investigators
Collaborators
Contributors
Resource Providers
Publishers
Acknowledgments
  • Funding Information: NOAA Ocean Acidification Program (Microbial relationships to ocean acidification chemistry in the northern California current ecosystem, OAP/NRDD project number 21405)
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science Keywords Provider Keywords
  • Cryptophytes
  • Prochlorococcus
  • Synechococcus
  • picophytoplankton
Provider Variable Abbreviations
  • Bacteria_total_cell_per_ml
  • Big_diatoms_cell_per_ml
  • Cryptophytes_cell_per_ml
  • Euk_phytoplankton_cell_per_ml
  • Prochlorococcus_cell_per_ml
  • Synechococcus_cell_per_ml
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Platform keywords NODC PLATFORM NAMES THESAURUS Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Geographic Names
  • West Coast of CONUS and British Columbia (Canada), partial
Project keywords NODC PROJECT NAMES THESAURUS Cruise ID
  • WCOA2016
EXPOCODE
  • 33RO20160505
Ocean Acidification Search Keywords
  • Ocean Acidification Program (OAP)
  • Ocean Carbon and Acidification Data System (OCADS) Project
Reference Section ID
  • Lines 8 - 17
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Rhodes, Linda D.; Adams, Nicolaus G.; Alin, Simone R.; Feely, Richard A. (2022). Cellular abundances of bacterioplankton and eukaryotic picoplankton measured by flow cytometry in water samples collected on NOAA Ship Ronald H. Brown during the West Coast Ocean Acidification Cruise led by the Pacific Marine Environmental Laboratory (PMEL) in the northern California current ecosystem from 2016-05-24 to 2016-06-16 (NCEI Accession 0265154). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0265154. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2022-10-18T20:33:18Z - NCEI Accession 0265154 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Parameter or Variable: Synechococcus abundance; Abbreviation: Synechococcus_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Synechococcus; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes
  • Parameter or Variable: Eukarotic picophytoplankton abundance; Abbreviation: Euk_phytoplankton_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Prymnesiophyceae, Chrysophyceae, Cryptophyceae, Pelagophyceae, Pinguiophyceae,Chlorarachniophyceae, Eustigmatophyceae, Pavlovophyceae, Trebouxiophyceae, Prasnophyceae; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes
  • Parameter or Variable: Large diatom (non-chain-forming) abundance; Abbreviation: Big_diatoms_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Bacillariophyceae; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes
  • Parameter or Variable: Prochlorococcus abundance; Abbreviation: Prochlorococcus_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Prochlorococcus; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes
  • Parameter or Variable: Cryptophyte abundance; Abbreviation: Cryptophytes_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Sample gating based on forward scatter, side scatter, orange fluorescence (564-606 nm), and long band-pass red fluorescence (>670 nm), data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: Robert J. Olson, Erik R. Zettler, Michele D. DuRand. Phytoplankton Analysis Using Flow Cytometry (Chapter 22). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Cryptophyceae; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes
  • Parameter or Variable: Total bacteria and archaea abundance; Abbreviation: Bacteria_total_cell_per_ml; Unit: cell per ml; Observation type: Counts by flow cytometry; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: BD 4-color FACSCalibur; Detailed sampling and analyzing information: Single 2L sample collected from Niskin bottle, duplicate 3 ml aliquots removed for preservation with 60µl of 10% paraformaldehyde, flash-frozen in liquid nitrogen, and transferred to -80°C longer term storage. Flow cytometer calibrated with 1.0 and 3.0 Floresbrite, and 6.0 µm TruCount beads as appropriate for sample volume determination and size gating. Cellular double-stranded nucleic acid stained with SYBR Green. Sample gating based on forward scatter, side scatter and green fluorescence (515 - 545 nm). Data collection for 1000000 events or 180 seconds with 200 ms resolution.; Replicate information: Duplicate; Quality flag convention: Cell count flag used for all flow cytometry samples. 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: D. K. Button, B. R. Robertson. Use of High-Resolution Flow Cytometry to Determine the Activity and Distribution of Aquatic Bacteria (Chapter 21). IN: Kemp, P.F., Cole, J.J., Sherr, B.F., and Sherr, E.B. (Eds.). (1993). Handbook of Methods in Aquatic Microbial Ecology (1st ed.). CRC Press. https://doi.org/10.1201/9780203752746; Biological subject: Bacteria, Archaea; Researcher name: Maria Kavanaugh; Researcher institution: Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (under contract to NOAA Fisheries); PI: Linda Rhodes
Acquisition Information (collection)
Instrument
  • Flow Cytometer
Platform
  • NOAA Ship Ronald H. Brown
Last Modified: 2025-04-04T07:52:08Z
For questions about the information on this page, please email: ncei.info@noaa.gov