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Dataset Overview | National Centers for Environmental Information (NCEI)

Biological effects of ocean acidification on juvenile Atlantic surfclams (Spisula solidissima) observed in laboratory experiments from 2019-04-10 to 2019-07-03 (NCEI Accession 0245952)

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This dataset contains laboratory experimental data that were collected to examine the effects of elevated levels of carbon dioxide on the growth of Atlantic surfclam (Spisula solidissima), a species that supports both commercial and recreational fisheries in the Northeast United States. Three levels of carbon dioxide enrichment (low, medium, and high) were delivered to surfclams in a 12-week exposure experiment. All treatments were done in 3 replicates (A, B, C). Approximately every 2 to 3 weeks, 12 individuals were removed from each treatment and measurements of length, width, height, dry tissue, and dry shell were recorded. Length was measured across the longest part of the shell, parallel to the hinge. Width was the thickness of the shell, and height was measured form the hinge to the outer edge of the shell. Dry tissue and dry shell samples were dried at 60°C until constant weight was achieved (~5 days). DIC measurements of carbon dioxide enrichment were taken and analyzed on an Apollo SciTech, while pH was measured weekly with a spectrophotometer. Values reported for DIC, pH, temperature, and salinity are the mean of each treatment during the 12-week experiment. The data indicated that increased carbon dioxide affected growth, tissue mass, and shell weight for Atlantic surfclam.
  • Cite as: Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Wikfors, Gary H.; Meseck, Shannon L. (2022). Biological effects of ocean acidification on juvenile Atlantic surfclams (Spisula solidissima) observed in laboratory experiments from 2019-04-10 to 2019-07-03 (NCEI Accession 0245952). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/fesj-kv54. Accessed [date].
gov.noaa.nodc:0245952
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2019-04-10 to 2019-07-03
Spatial Bounding Box Coordinates
West: -73.051877
East: -73.051877
South: 41.211919
North: 41.211919
Spatial Coverage Map
General Documentation
Associated Resources
Publication Dates
  • publication: 2022-01-28
  • revision: 2022-03-09
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
This data package (Submission ID: BFUVKZ3TD) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL.

In this accession, NCEI has archived multiple versions of these data. The latest (and best) version of these data has the largest version number.
Purpose To determine if increase carbon dioxide would effect growth, tissue weight, and shell weight of Atlantic surfclams.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Wikfors, Gary H.; Meseck, Shannon L. (2022). Biological effects of ocean acidification on juvenile Atlantic surfclams (Spisula solidissima) observed in laboratory experiments from 2019-04-10 to 2019-07-03 (NCEI Accession 0245952). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/fesj-kv54. Accessed [date].
Cited Authors
Principal Investigators
Contributors
Resource Providers
Publishers
Acknowledgments
  • Funding Information: NOAA Ocean Acidification Program (OA Growth Affects on Eastern oysters Atlantic surfclams and Atlantic sea scallops (Meseck NEFSC), 17911)
  • Funding Information: NOAA OAP (“Is Scope for Growth of Eastern oysters, Crassostrea virginica, Atlantic surfclams, Spisula solidissima, and Atlantic sea scallops, Placopecten magellanicus, affected by ocean acidification?”)
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science Keywords OCADS Study Type
  • Laboratory experiment
Provider Keywords
  • Atlantic surfclam
  • Spisula solidissima
Provider Variable Abbreviations
  • DIC
  • H
  • L
  • SM
  • Salinity
  • TM
  • Temperature
  • W
  • exposure
  • pCo2
  • pH
  • pH (in situ)
  • replicate letter
  • ΩC
  • Ωa
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords NODC INSTRUMENT TYPES THESAURUS
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Geographic Names
  • CT
  • Milford
Project keywords NODC PROJECT NAMES THESAURUS Ocean Acidification Search Keywords
  • Ocean Acidification Program (OAP)
  • Ocean Carbon and Acidification Data System (OCADS) Project
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Wikfors, Gary H.; Meseck, Shannon L. (2022). Biological effects of ocean acidification on juvenile Atlantic surfclams (Spisula solidissima) observed in laboratory experiments from 2019-04-10 to 2019-07-03 (NCEI Accession 0245952). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/fesj-kv54. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2022-01-28T22:26:22Z - NCEI Accession 0245952 v1.1 was published.
  • 2022-03-09T22:04:18Z - NCEI Accession 0245952 was revised and v2.2 was published.
    Rationale: Updates were received for this dataset. These updates were copied into the data/0-data/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Polypropylene bottle; Analyzing instrument: Discrete dissolved inorganic carbon (DIC) measurement (Non-dispersive infrared, or NDIR); Detailed sampling and analyzing information: DIC samples were analyzed on an Apollo SciTech DIC analyzer (Apollo SciTech, LLC, Newark, DE).Precision of the DIC instrument was confirmed by an international interlaboratory comparison exercise that consisted of low and high CO2 test seawater samples, with the laboratory being within 0.5% of assigned values (Bockmon and Dickson, 2015). During analysis, replicate measurements (n = 3) on a certified reference material were within ±2.7 μmol kg− 1 of the reported value.; Replicate information: Triplicate samples; Standardization description: CRMs; Standardization frequency: daily; CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography.; Preservation method: run within minutes of collection; Preservative volume: none; Preservative correction: none; Uncertainty: ±2.7 μmol kg− 1; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS.
  • Parameter or Variable: pH; Abbreviation: pH; pH scale: seawater scale; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Polypropylene bottle; Analyzing instrument: Spectrophotometer; Detailed sampling and analyzing information: Total pH was determined colorimetrically at 20 ◦C using a metacresol purple indicator dye (Sigma-Aldrich, St. Louise, MS, Dickson and Goyet, 1994) with a UV-VIS spectrophotometer (Cary100, Agilent, Santa Clara, CA, USA). Tris-buffer was used to ensure accuracy of the measurements.; Replicate information: Triplicate samples; Standardization description: TRIS BUFFER, Andrew Dickson Acid; Standardization frequency: weekly; Temperature of standardization: 20 ◦C; Temperature correction method: water bath; At what temperature was pH reported: 20 ◦C; Uncertainty: ± 0.0014.
  • Parameter or Variable: exposure; Abbreviation: exposure; Unit: days; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: None; Sampling instrument: None; Analyzing instrument: NOne; Duration: 12 weeks; Detailed sampling and analyzing information: Exposure is the number of days that the animal was exposed to carbon dioxide.; Method reference: None; Biological subject: Atlantic Surfclam; Species ID: 80945; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS.
  • Parameter or Variable: pH; Abbreviation: pH (in situ); Unit: ph seawater scale; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Calculation method and parameters: CO2SYS; Sampling instrument: Polypropylene bottle; Analyzing instrument: Spectrophotometer; Duration: 12 weeks; Detailed sampling and analyzing information: Calculated pH at in situ temperature; Replicate information: Triplicate samples; Biological subject: Atlantic surfclam; Species ID: 80945; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS.
  • Parameter or Variable: Salinity; Abbreviation: Salinity; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: YSI - handheld multi-parameter instrument; Analyzing instrument: YSI - handheld multi-parameter instrument; Duration: 12 weeks; Detailed sampling and analyzing information: Daily water variables (temperature, salinity) were measured with a YSI probe (Model 556, Yellowspring, OH USA); Uncertainty: 0.1; Biological subject: Atlantic Surfclam; Species ID: 80945.
  • Parameter or Variable: water temperature; Abbreviation: Temperature; Unit: C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: YSI - handheld multi-parameter instrument; Analyzing instrument: YSI - handheld multi-parameter instrument; Duration: 12 weeks; Detailed sampling and analyzing information: Daily water variables (temperature, salinity) were measured with a YSI probe (Model 556, Yellowspring, OH USA); Uncertainty: 0.1; Biological subject: Atlantic Surfclam; Species ID: 80945.
  • Parameter or Variable: partial pressure (or fugacity) of carbon dioxide - water; Abbreviation: pCo2; Unit: uatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Calculation method and parameters: CO2 sys; Duration: 12 week; Detailed sampling and analyzing information: DIC and pH were used in CO2SYS (Pierrot et al., 2006) for the calculation of ρCO2 (μatm), ΩCalcite, and Ωaragonite using the following constants: K1, K2 from Mehrbach et al. (1973) refit by Dickson and Millero (1987); K hydrogen sulfate from Dickson et al. (1990); and total Boron from Uppstrom 1974).; Biological subject: Atlantic Surfclam; Species ID: 80945.
  • Parameter or Variable: calcite saturation state; Abbreviation: ΩC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Calculation method and parameters: CO2sys; Duration: 12 week; Detailed sampling and analyzing information: DIC and pH were used in CO2SYS (Pierrot et al., 2006) for the calculation of ρCO2 (μatm), ΩCalcite, and Ωaragonite using the following constants: K1, K2 from Mehrbach et al. (1973) refit by Dickson and Millero (1987); K hydrogen sulfate from Dickson et al. (1990); and total Boron from Uppstrom (1974).; Biological subject: Atlantic surflcam; Species ID: 80945.
  • Parameter or Variable: aragonite saturation state; Abbreviation: Ωa; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Calculation method and parameters: co2sys; Duration: 12 week; Detailed sampling and analyzing information: DIC and pH were used in CO2SYS (Pierrot et al., 2006) for the calculation of ρCO2 (μatm), ΩCalcite, and Ωaragonite using the following constants: K1, K2 from Mehrbach et al. (1973) refit by Dickson and Millero (1987); K hydrogen sulfate from Dickson et al. (1990); and total Boron from Uppstrom (1974).; Biological subject: Atlantic Surfclam; Species ID: 80945.
  • Parameter or Variable: treatment letter; Abbreviation: replicate letter; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: none; Duration: 12 week; Detailed sampling and analyzing information: This is the container number for each treatment. Each level of carbon dioxide had 3 replicates identified as A, B, C. The initial measurements at day zero would apply to all treatments.; Biological subject: Atlantic surfclams; Species ID: 80945.
  • Parameter or Variable: shell parameters (length or weight); Abbreviation: L; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Traceable digital calipers calibrated; Analyzing instrument: calipers; Duration: 12 week; Detailed sampling and analyzing information: Length was measured across the longest part of the shell. Parallel to the hinge; Biological subject: Atlantic surfclam; Species ID: 80945.
  • Parameter or Variable: width of bivalve; Abbreviation: W; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Traceable Digital Caliper with Calibration; Analyzing instrument: Caliper; Duration: 12 week; Detailed sampling and analyzing information: Width of the bivalve was measured as the thickness of the organism where the shells are joined together.; Biological subject: Atlantic surfclam; Species ID: 80945.
  • Parameter or Variable: height; Abbreviation: H; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Traceable Digital Caliper with Calibration; Analyzing instrument: Calipers; Duration: 12 week; Detailed sampling and analyzing information: Height was measured from the hinge to the outer edge of the shell.; Biological subject: Atlantic surfclam; Species ID: 80945.
  • Parameter or Variable: dry weight tissue mass; Abbreviation: TM; Unit: g; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Balance; Analyzing instrument: Balance; Duration: 12 week; Detailed sampling and analyzing information: Tissue was dried at 60C to constant weight then dry tissue weight was measured.; Biological subject: Atlantic surfclam; Species ID: 80945.
  • Parameter or Variable: shell dry mass; Abbreviation: SM; Unit: g; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Balance; Analyzing instrument: Balance; Duration: 12 weeks; Detailed sampling and analyzing information: Shell were dried until constant weight at 60C then weighed on a balance to get dry shell mass; Biological subject: Atlantic surfclam; Species ID: 80945 .
Acquisition Information (collection)
Instrument
  • bottle
  • calipers
  • multi-parameter water quality sensor
  • scale
Last Modified: 2024-06-10T13:21:28Z
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