Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040)
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This dataset contains benthic and pelagic invertebrate stable carbon and nitrogen isotope data collected in the Chukchi Sea, U.S. Arctic during the 7 August 2017 - 22 August 2017 Arctic Marine Biodiversity Observing Network (AMBON) research cruise aboard the vessel Norseman II. The dataset contains a comma separated values (csv) files exported from Microsoft Excel. These data were generated from samples collected with trawls, grabs, and plankton nets during the research cruise. The data in the file named AMBON2017_Stable Isotopes_Database_final.csv describe the carbon and nitrogen stable isotope values of invertebrate samples with and without various chemical treatments to eliminate carbonates and lipids. Full location and taxonomic information is given for each sample.
- Cite as: Iken, Katrin (2021). Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/jx3j-4s60. Accessed [date].
Time Period |
2017-08-07 to 2017-08-22 |
Spatial Bounding Box Coordinates |
West: -168.9543
East: -159.6922
South: 67.668
North: 72.496
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Spatial Coverage Map |
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General Documentation |
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Associated Resources |
- How to account for the lipid effect on carbon stable-isotope ratio (δ13C): sample treatment effects and model bias
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Publication Dates |
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Data Presentation Form |
Digital table - digital representation of facts or figures systematically displayed, especially in columns
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Dataset Progress Status |
Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency |
As needed |
Supplemental Information |
Submission Package ID: DBBNBY |
Purpose |
The goal of the Arctic Marine Biodiversity Observing Network (AMBON) project is to demonstrate and build an operational marine biodiversity observing network from microbes to whales, integrating diversity levels from genetic to organismal. AMBON is funded through the National Ocean Partnership Program, with contributions from the National Oceanographic and Atmospheric Administration (NOAA), the Bureau of Ocean and Energy Management (BOEM), Shell Exploration and Production Company, and the National Science Foundation (NSF). The AMBON field region is located on the Chukchi Sea continental shelf in the US Arctic as a region exposed to climatic changes and anthropogenic influences. AMBON is composed of a team of multi-institutional and multi-sector partners active in a variety of Arctic biodiversity observing programs and covering disciplines ranging from genetic to organismal to ecosystem. The purpose of this dataset is to describe the stable isotope values of benthic and pelagic invertebrates (with variable treatment to exclude carbonates and lipids) to be used in food web studies and identify carbon sourcing. |
Use Limitations |
- Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
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Dataset Citation |
- Cite as: Iken, Katrin (2021). Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/jx3j-4s60. Accessed [date].
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Cited Authors |
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Principal Investigators |
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Contributors |
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Resource Providers |
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Publishers |
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Acknowledgments |
- Related Funding Agency: National Ocean Partnerships Program
- Related Funding Agency: National Science Foundation
- Related Funding Agency: Shell Development Co.
- Related Funding Agency: National Oceanic and Atmospheric Administration
- Related Funding Agency: US DOI; Bureau of Ocean Energy Management
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Theme keywords |
NODC DATA TYPES THESAURUS
NODC OBSERVATION TYPES THESAURUS
WMO_CategoryCode
Global Change Master Directory (GCMD) Science Keywords
Provider Data Types
- CARBON - ORGANIC (Measured)
- NITROGEN - ORGANIC (Measured)
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Data Center keywords |
NODC COLLECTING INSTITUTION NAMES THESAURUS
NODC SUBMITTING INSTITUTION NAMES THESAURUS
Global Change Master Directory (GCMD) Data Center Keywords
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Platform keywords |
NODC PLATFORM NAMES THESAURUS
Global Change Master Directory (GCMD) Platform Keywords
ICES/SeaDataNet Ship Codes
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Instrument keywords |
NODC INSTRUMENT TYPES THESAURUS
Global Change Master Directory (GCMD) Instrument Keywords
Provider Instruments
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Place keywords |
NODC SEA AREA NAMES THESAURUS
Global Change Master Directory (GCMD) Location Keywords
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Project keywords |
Provider Project Names- Arctic Marine Biodiversity Ocean Network (AMBON)
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Keywords |
NCEI ACCESSION NUMBER
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Use Constraints |
- Cite as: Iken, Katrin (2021). Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/jx3j-4s60. Accessed [date].
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Access Constraints |
- Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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Fees |
- In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
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Lineage information for: dataset |
Processing Steps |
- 2021-07-29T14:38:12Z - NCEI Accession 0239040 v1.1 was published.
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Output Datasets |
- NCEI Accession 0239040 v1.1
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Lineage information for: dataset |
Processing Steps |
- Parameter or Variable: TAXONOMIC CODE (measured); Units: N/A; Observation Category: laboratory analysis; Sampling Instrument: net - zooplankton net; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh..
- Parameter or Variable: NITROGEN - ORGANIC (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: various; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh. Organisms were sampled as whole organisms (most zooplankton), whole organisms but with the intestinal tract removed (most amphipods), or a slow-turnover tissue such as muscle or body wall was selected. For echinoderms with little muscle tissue, tube feet (Asteroidea) or oral discs (Ophiuroidea) were sampled. Most samples were collected as individuals in replicates of three, but in cases of small individuals, numerous individuals were pooled (e.g., zooplankton) for sufficient mass. Tissue samples were dried at 60°C until constant weight and stored dry until analysis. All taxon names were standardized to the World Register of Marine Species (www.marinespecies.org) Samples were analyzed after one of multiple of the following treatments; many samples were analyzed after several of these treatments to assess treatment effects for the different species: 1.“no treatment” indicates that a dried sample was not chemically treated in any way before isotope analysis; 2.“HCl” indicates a sample was exposed to 1N HCl repeatedly until bubbling from carbonate dissolution ceased to remove isotopically heavier inorganic carbonates from the dietary organic carbon pool (Schlacher & Connolly 2014); 3.“CM” indicates a sample was repeatedly treated with 2:1 chloroform:methanol (CM) to remove 13C-depleted lipids (Mintenbeck et al. 2008) 4.“HCl+CM” indicates samples that were first treated with HCl to remove carbonates and the treated with 2:1 chloroform:methanol to remove lipids. All tissues were rinsed and re-dried after chemical treatment before analysis for carbon and nitrogen stable isotope analysis at the Alaska Stable Isotope Facility (ASIF) at UAF. Stable isotope data for all samples were obtained using continuous-flow isotope ratio mass spectrometry (CF-IRMS). Approximately 0.3-0.5 mg dry weight of each faunal tissue sample were analyzed for delta13C and delta15N values. using a Costech ESC 4010 elemental analyzer interfaced via a Conflo IV with an IRMS (Thermo Finnigan Delta VPlus). Results are expressed as conventional delta (δ) notation in parts per thousand (‰) according to the following equation: delta X = [(R sample/R standard) – 1] • 1000 where X is 13C or 15N of the sample and R is the corresponding ratio 13C/12C or 15N/14N with standards of Vienna Pee Dee Belemnite for delta13C and atmospheric N2 for delta15N. Stable isotope analysis also provided carbon and nitrogen concentrations (as percent) of the sample; these concentrations are given for each measurement as it allows calculation of C;N ratios of samples, which is a useful tool in food web studies and in mathematical corrections of lipid content in samples.; Data Quality Method: delta 13C and delta 15N values accuracy was <0.1 per mil standard deviation.
- Parameter or Variable: CARBON - ORGANIC (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: various; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh. Organisms were sampled as whole organisms (most zooplankton), whole organisms but with the intestinal tract removed (most amphipods), or a slow-turnover tissue such as muscle or body wall was selected. For echinoderms with little muscle tissue, tube feet (Asteroidea) or oral discs (Ophiuroidea) were sampled. Most samples were collected as individuals in replicates of three, but in cases of small individuals, numerous individuals were pooled (e.g., zooplankton) for sufficient mass. Tissue samples were dried at 60°C until constant weight and stored dry until analysis. All taxon names were standardized to the World Register of Marine Species (www.marinespecies.org) Samples were analyzed after one of multiple of the following treatments; many samples were analyzed after several of these treatments to assess treatment effects for the different species: 1.“no treatment” indicates that a dried sample was not chemically treated in any way before isotope analysis; 2.“HCl” indicates a sample was exposed to 1N HCl repeatedly until bubbling from carbonate dissolution ceased to remove isotopically heavier inorganic carbonates from the dietary organic carbon pool (Schlacher & Connolly 2014); 3.“CM” indicates a sample was repeatedly treated with 2:1 chloroform:methanol (CM) to remove 13C-depleted lipids (Mintenbeck et al. 2008) 4.“HCl+CM” indicates samples that were first treated with HCl to remove carbonates and the treated with 2:1 chloroform:methanol to remove lipids. All tissues were rinsed and re-dried after chemical treatment before analysis for carbon and nitrogen stable isotope analysis at the Alaska Stable Isotope Facility (ASIF) at UAF. Stable isotope data for all samples were obtained using continuous-flow isotope ratio mass spectrometry (CF-IRMS). Approximately 0.3-0.5 mg dry weight of each faunal tissue sample were analyzed for delta13C and delta15N values. using a Costech ESC 4010 elemental analyzer interfaced via a Conflo IV with an IRMS (Thermo Finnigan Delta VPlus). Results are expressed as conventional delta (δ) notation in parts per thousand (‰) according to the following equation: delta X = [(R sample/R standard) – 1] • 1000 where X is 13C or 15N of the sample and R is the corresponding ratio 13C/12C or 15N/14N with standards of Vienna Pee Dee Belemnite for delta13C and atmospheric N2 for delta15N. Stable isotope analysis also provided carbon and nitrogen concentrations (as percent) of the sample; these concentrations are given for each measurement as it allows calculation of C;N ratios of samples, which is a useful tool in food web studies and in mathematical corrections of lipid content in samples.; Data Quality Method: delta 13C and delta 15N values accuracy was <0.1 per mil standard deviation.
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Acquisition Information (collection) |
Instrument |
- net - zooplankton net
- trawl
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Platform |
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Last Modified: 2024-06-10T13:19:06Z
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