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OAS accession Detail for 0278522
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| accessions_id: | 0278522 | archive |
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| Title: | Chemistry and cell counts of formation fluids from North Pond, western flank of the Mid-Atlantic Ridge, from 2012-2014 (NCEI Accession 0278522) |
| Abstract: | This dataset contains biological, chemical, and survey - biological data collected on R/V Maria S. Merian during cruises MSM20-5 and MSM37 in the North Atlantic Ocean from 2012-04-20 to 2014-04-10. These data include Nitrate, Si, abundance, and dissolved Oxygen. The instruments used to collect these data include Aanderaa Oxygen Optodes, Automatic titrator, CTD profiler, GeoMICROBE, and Microscope-Fluorescence. These data were collected by Peter Girguis of Harvard University, Julie Huber of Marine Biological Laboratory, and Brian Glazer of University of Hawaii at Manoa as part of the "Collaborative Research: Characterization of Microbial Transformations in Basement Fluids, from Genes to Geochemical Cycling (North Pond Microbes)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" and "International Ocean Discovery Program (IODP)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-12-02. The following is the text of the dataset description provided by BCO-DMO: Chemistry and cell counts of formation fluids from North Pond Dataset Description: Acquisition Description: Sample collection Crustal fluids were collected from the single horizon at U1382A and from the shallow, middle and deep horizons in U1383C (Edwards et al., 2012) using a mobile pumping system designed for microbial sampling from CORK fluid delivery lines as described in Meyer et al. (2016) and Cowen et al. (2012). Deployed with the ROV system, mobile pumping system connectors are attached to the CORK wellhead via an umbilical to the hydrological zone of interest within the aquifer. Fluid systems were flushed and allowed to equilibrate before sampling, and dissolved oxygen concentrations were measured during pumping using an Aanderaa sensor (Meyer et al., 2016). In 2012, 12 l of each fluid sample were filtered on to a 0.22 μm Sterivex-GP filter (Merck Millipore, Billerica, MA, USA) as described in Meyer et al. (2016). In 2014, 12 l of each sample was filtered in situ and immediately fixed with RNALater (Thermo Fisher Scientific, Waltham, MA, USA), as described previously (Akerman et al., 2013). After sampling in 2012, a battery-powered GeoMICROBE sled was left at each CORK for time series autonomous sampling of the fluid delivery lines (Cowen et al., 2012). For each filter sample, ~10 l of fluid were filtered in situ and immediately fixed with RNALater. For downstream analysis, ~500 ml of fluid were filtered into two Tedlar bags, one containing 54 ml of 37% formaldehyde for cell enumeration and the other with 4 ml of 10% HCl for inorganic chemistry analyses. Sleds were deployed in April 2012 and recovered in April 2014 with samples collected. Upon sled recovery, filters were transferred to fresh RNALater and stored at −80 °C, while all bag samples were stored at 4 °C (Cowen et al., 2012). Deep bottom water was sampled in 2012 and 2014 via a CTD at 100 m above the seafloor and filtered in the same manner as the crustal fluids onto Sterivex filters. Total microbial biomass in fluids was enumerated with DAPI (4′,6′-diamidino-2-phenylindole; Sigma-Aldrich, St Louis, MO, USA) and epifluorescent microscopy (Porter and Feig, 1980). Fluids also were analyzed for dissolved silicon and nitrate using automated colorimetric analysis and pH was measured with an electrode before a potentiometric titration for the determination of alkalinity (Wheat et al., 2017). |
| Date received: | 20191202 |
| Start date: | 20120420 |
| End date: | 20140410 |
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| West boundary: | -46.09 |
| East boundary: | -46.05 |
| North boundary: | 22.8 |
| South boundary: | 22.78 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Metadata version: | 1 |
| Keydate: | 2023-05-20 04:51:17+00 |
| Editdate: | 2023-05-20 04:51:53+00 |