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OAS accession Detail for 0278347
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| accessions_id: | 0278347 | archive |
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| Title: | Coral colony genetic sequence accession numbers for samples collected from the lagoon environment of Ofu Island, American Samoa between 2011 and 2015 (NCEI Accession 0278347) |
| Abstract: | This dataset contains biological and survey - biological data collected at American_Samoa during deployment Palumbi_AmSamoa_2013-2015 in the South Pacific Ocean from 2011-04-01 to 2015-12-01. These data include species. The instruments used to collect these data include Automated DNA Sequencer. These data were collected by Dr Stephen R. Palumbi of Stanford University and Luke Thomas of University of Western Australia as part of the "Ecological, evolutionary and physiological responses of corals to a mass bleaching event in American Samoa (Bleaching American Samoa)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-04-12. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Coral colony genetic sequence accessions for samples collected from the lagoon environment of Ofu Island in the National Park of American Samoa between 2011 and 2015. All data and samples were collected at depths less than 3.0m. This dataset includes all sequence data accession numbers for the publication Thomas & Palumbi (2017) housed at The National Center for Biotechnology Information under BioProject PRJNA522016 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522016). |
| Date received: | 20190412 |
| Start date: | 20110401 |
| End date: | 20151201 |
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| West boundary: | -169.655 |
| East boundary: | -169.655 |
| North boundary: | -14.18 |
| South boundary: | -14.18 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Seven colonies of A. hyacinthus from the back-reef environment of Ofu Island were tagged, sampled, and monitored at five time-points spanning the bleaching event: before bleaching representing baseline expression levels (August 2011), and then two months (April 2015), six months (August 2015), 10 months (December 2015) and 12 months (February 2016) after initial bleaching was observed in February 2015. Coral nubbins of approximately 24 cm in size were collected from colonies within 3 h of high-tide using garden clippers and preserved in RNAlater. All colonies occurred at depths of approximately 0.51.0 m and were within a 2500 m2area of back-reef. Total RNA was extracted from tissue samples using Qiagen's RNAeasy Plus Kit, and 35 cDNA libraries (seven colonies for five dates) were generated using the Illumina TruSeq RNA Library Prep Kit v2 with Protoscript II Reverse Transcriptase. The 35 libraries were multiplexed and sequenced across three lanes on a Hiseq2500 at the University of Utah Microarray and Genomic Analysis Core Facility. Location: Ofu Island, American Samoa. All data and samples were collected from the lagoon environment in the National Park of American Samoa at depths less than 3.0m. |
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| Metadata version: | 1 |
| Keydate: | 2023-05-17 12:55:53+00 |
| Editdate: | 2023-05-17 12:56:27+00 |