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OAS accession Detail for 0277423
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Title: Larval mussel shell composition under high and low pH and O2 from SIO, UCSD from 2012 and 2013 (OA_Proxies project) (NCEI Accession 0277423)
Abstract: This dataset contains biological and survey - biological data collected at SIO Levin during deployment Levin_mussels from 2012-05-05 to 2013-01-23. These data include species. The instruments used to collect these data include Inductively Coupled Plasma Mass Spectrometer. These data were collected by Lisa A Levin of University of California-San Diego as part of the "Development of geochemical proxies to evaluate larval pH-exposure history (OA_Proxies)" project and "Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.

The following is the text of the dataset description provided by BCO-DMO:

Larval mussel shell composition under high and low pH and O2

Dataset Description:
Larval mussel shell composition under high and low pH and O2 from SIO, UCSD from 2012 and 2013.
Date received: 20230123
Start date: 20120505
End date: 20130123
Seanames:
West boundary: -119.842
East boundary: -117.203
North boundary: 34.4128
South boundary: 32.725
Observation types:
Instrument types:
Datatypes:
Submitter:
Submitting institution: Biological and Chemical Oceanography Data Management Office
Collecting institutions:
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Number of observations:
Supplementary information: Acquisition Description:
Mussel larvae were obtained and cultured as described in Frieder et al. 2014, EST. At the end of each experiment, the larvae were frozen then cleaned from organic material using trace-metal-clean techniques in a clean room (described in Frieder et al. 2014, EST).

Once clean and mounted, larvae were individually analyzed for a combination of elements (Mg, Cu, Zn, Sr, Ba, Pb, U with respect to Ca) at UC Santa Barbara using a New Wave research UP-213 laser ablation unit coupled with a Thermo Element 2 single-collector inductively coupled plasma mass spectrometer operating in low resolution mode.

As described in the supplemental material for Frieder et al. 2014 (EST) "three standards were used to ensure proper calibration of the instrument: a solution-based dissolved CaCO3 reference material (OTO, [Spex certified primary standard solutions]), and two solid standards, NIST 612 and USGS MACS3 CaCO3 standard. All reference materials, both solution- and solid-based, were analyzed at the beginning and the end of each run; mounting medium[, solution standards] and instrument blanks were run multiple times during a sequence. For ablations, laser settings were a single, 30-µm-long by 80-µm-wide line at 40% power, 10 Hz, and 8 µm s-1 scanning speed. These settings usually consumed the entire larval shell. The tape used to mount the shells had less than 5% of the average shell value for each element."
Availability date:
Metadata version: 1
Keydate: 2023-04-03 05:33:26+00
Editdate: 2023-04-03 05:35:22+00