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OAS accession Detail for 0278775
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| accessions_id: | 0278775 | archive |
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| Title: | Biogeochemistry of microbial phosphorus uptake from cruises in the Sargasso Sea; Bermuda Atlantic Time-Series Station from 2011-2013 (Biological C:N:P ratios project) (NCEI Accession 0278775) |
| Abstract: | This dataset contains biological, chemical, and survey - biological data collected on R/V Atlantic Explorer during cruises AE1123, AE1206, and AE1319 in the Labrador Sea and North Atlantic Ocean from 2011-09-28 to 2013-09-08. These data include Nitrite, Silicate, chlorophyll a, depth, nitrate plus nitrite, particulate organic Carbon (POC), particulate organic nitrogen, prochlorococcus abundance, reactive phosphorus (PO4), and synechococcus abundance. The instruments used to collect these data include Flow Cytometer, Niskin bottle, Nutrient Autoanalyzer, and Plankton Net. These data were collected by Michael W. Lomas of Bigelow Laboratory for Ocean Sciences, Simon Levin of Princeton University, Adam Martiny of University of California-Irvine, and Dr Juan A. Bonachela of University of Strathclyde as part of the "Biological Controls on the Ocean C:N:P ratios (Biological C:N:P ratios)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" and "Ocean Carbon and Biogeochemistry (OCB)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-12-03. The following is the text of the dataset description provided by BCO-DMO: Bulk phosphorus uptake by microbes Dataset Description: Biogeochemistry of microbial phosphorus uptake from cruises in the Sargasso Sea; Bermuda Atlantic Time-Series Station from 2011-2013 (Biological C:N:P ratios project).This data was published in Lomas et al. 2014 PNAS. |
| Date received: | 20201203 |
| Start date: | 20110928 |
| End date: | 20130908 |
| Seanames: | Labrador Sea, North Atlantic Ocean |
| West boundary: | -65.967 |
| East boundary: | -45 |
| North boundary: | 55 |
| South boundary: | 19.667 |
| Observation types: | biological, chemical, survey - biological |
| Instrument types: | Flow Cytometer, net - plankton net, Niskin bottle, nutrient autoanalyzer |
| Datatypes: | CHLOROPHYLL A, DEPTH - OBSERVATION, nitrate + nitrite content (concentration), NITRITE, PARTICULATE ORGANIC CARBON, PARTICULATE ORGANIC NITROGEN (PON), phosphate, Prochlorococcus, silicate, species abundance, Synechococcus |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Bigelow Laboratory for Ocean Sciences, Princeton University, University of California - Irvine |
| Contributing projects: | |
| Platforms: | Atlantic Explorer (33H4) |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Sample collection: The data presented in this study were collected on 7 cruises throughout the Western North Atlantic Ocean (cruise X0606, X0705, X0804, BVAL 39, BVAL 46, AE1206, and AE1319). Data for kinetics experiments were collected from throughout the western North Atlantic, from roughly 55oN in the Labrador Sea to ~21oN, just north of Puerto Rico. All sample depths were Dissolved inorganic and organic, and particulate nutrients: Samples for NO3-/NO2-, NO2- and PO4-3 are gravity filtered through 0.8 µm Nucleopore polycarbonate filters using acid cleaned in-line polycarbonate filter holders, then frozen (-20oC) in HDPE bottles until analysis (Dore et al., 1996). Tests of frozen versus refrigerated samples have indicated no significant difference between storage methods (Dore et al., 1996). Nutrient samples prior to ~2003 were analyzed on a modified Technicon Autoanalyzer and samples post ~2003 were analyzed on an Alpkem Flow Solution IV; both instrumental setups have comparable sensitivity and method detection limits validated by 6-month sample overlap on both instruments. During every sample run, commercially available certified standards, OSIL and Wako Chemical, are analyzed to maintain data quality, as well as ‘standard water’ from 3000 m which serves as an internal standard. Soluble reactive phosphate (SRP) concentrations in the euphotic zone of the Sargasso Sea are below analytical detection limits (~20 nmol kg-1) of standard nutrient autoanalyzer configurations. To resolve the low concentrations of SRP in the surface waters at BATS the Magnesium Induced Co-precipitation method, referred to as MAGIC-SRP measurements (Karl and Tien, 1992; Rimmelin and Moutin, 2005), was used starting in late 2004. Several modifications to the method were made and detailed in Lomas et al. (2010a). Sample accuracy was checked on each run with a certified OSIL nutrient standard. The method detection limit following this protocol is ~1 nmol kg-1 with a precision of + 5% at 5 nmol kg-1. Particulate organic carbon (POC) and nitrogen (PON) samples are filtered on precombusted (450oC, 4h) Whatman GF/F filters and frozen until analysis Steinberg et al., 2001a. Samples are analyzed on a Control Equipment 240-XA or 440-XA elemental analyzer standardized to acetanilide. Particulate phosphorus samples (PPhos) are analyzed using a ash-hydrolysis method (Lomas et al., 2010a). Oxidation efficiency and standard recovery is tested with each sample run using an ATP standard solution and a certified phosphate standard (OSIL Phosphate Nutrient Standard Solution). Method precision is ~9% at 2.5 nmol kg-1 (the lowest concentrations typically observed well below the euphotic zone), and ~1% at 15 nmol kg-1 (typical euphotic zone concentrations). The method detection limit, defined as three times the standard deviation of the lowest standard (2.5 nmol kg-1) is ~0.5 nmol kg-1. 33Phosphate incubations: The approach for ambient whole community and population-specific uptake rate measurements were previously published ( Casey et al, 2009 ). Briefly, duplicate aliquots of 10 ml seawater were amended with 0.15 µCi (~80 pmol L-1) additions of H333PO4 (3000 Ci mol-1; PerkinElmer, USA), and incubated for 30 - 60 min in subdued lighting (~100 µmol photons m-2 s-1) at ~23oC. This temperature was within ~3oC of the coolest/warmest in situ temperature from which the samples were collected. The duration of each incubation varied depending on turnover time of the added isotope, such that efforts were made to keep uptake to Whole community and population-specific kinetics experiments were conducted by adding 0.15 µCi (~80 pM) of H333PO4 to ~10 replicate 10 ml seawater samples that were further amended by increasing additions of ‘cold’ KH2PO4 up to 100 nM. Samples were incubated as above, but the incubations were terminated by the addition of KH2PO4 to a final concentration of 100 µM (28). Whole community samples were filtered onto 0.2 µm polycarbonate filters, and rinsed with an oxalate wash (29). Surface bound phosphate in population-specific samples was accounted for by subtracting 33P counts for sorted populations to which 100 µM phosphate had been added prior to addition of the isotopic tracer. It is assumed that addition of such a high level of phosphate would result in negligible uptake of radioactive phosphate and thus any signal was attributed to surface absorption; this correction was always Flow cytometry analysis and cell sorting: Samples were sorted on an InFlux cell sorter (BD, Seattle, WA) at an average flow rate of ~40 µL min-1. Samples were sorted for Prochlorococcus , Synechococcus , and an operationally defined eukaryotic algae size fraction (eukaryotes >2 µm). A 100 mW blue (488 nm) excitation laser was used. After exclusion of laser noise gated on pulse width and forward scatter, autotrophic cells were discriminated by chlorophyll fluorescence (>650 nm), PE (585/30 nm), and granularity (side scatter). Sheath fluid was made fresh daily from distilled deionized water (Millipore, Billerica, MA) and molecular grade NaCl (Mallinckrodt Baker, Phillipsburg, NJ), pre-filtered through a 0.2 µm capsule filter (Pall, East Hills, NY), and a STERIVEX sterile 0.22 µm inline filter (Millipore, Billerica, MA). Mean coincident abort rates were |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-26 04:43:15+00 |
| Editdate: | 2023-05-26 04:43:57+00 |