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OAS accession Detail for 0291457
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| accessions_id: | 0291457 | archive |
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| Title: | N isotope data for individual amino acids from modern west Antarctic seals from 2008-01-01 to 2011-12-31 (NCEI Accession 0291457) |
| Abstract: | This dataset contains biological, chemical, and survey - biological data collected in the Ross Sea, South Atlantic Ocean, South Pacific Ocean, and Southern Ocean from 2008-01-01 to 2011-12-31. These data include common_name, d15N measured in biota, sex, taxon, and taxon_code. The instruments used to collect these data include Isotope-ratio Mass Spectrometer. These data were collected by A. Rus Hoelzel of Durham University, Daniel P. Costa and Paul L. Koch of University of California-Santa Cruz, and Brenda Hall of University of Maine as part of the "Collaborative Research: Exploring the Vulnerability of Southern Ocean Pinnipeds to Climate Change - An Integrated Approach (Southern Ocean Pinnipeds)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-08-22. The following is the text of the dataset description provided by BCO-DMO: N isotope data for individual amino acids from modern west Antarctic seals Dataset Description: These data are published and discussed in: Brault, E. (2017). An Examination of the Ecological and Oceanographic Effects of Mid-to-Late Holocene Climate Changes on the Ross Sea Ecosystem. UC Santa Cruz. ProQuest ID: Brault_ucsc_0036E_11435. Merritt ID: ark:/13030/m5dg1n5d. Retrieved from https://escholarship.org/uc/item/99s5j3fk |
| Date received: | 20220822 |
| Start date: | 20080101 |
| End date: | 20111231 |
| Seanames: | Ross Sea, South Atlantic Ocean, Southern Ocean, South Pacific Ocean |
| West boundary: | 162.684 |
| East boundary: | -60.77 |
| North boundary: | -62.47 |
| South boundary: | -77.652 |
| Observation types: | biological, chemical, survey - biological |
| Instrument types: | mass spectrometer |
| Datatypes: | DELTA NITROGEN-15, SPECIES IDENTIFICATION, SPECIES IDENTIFICATION - SEX, TAXONOMIC CODE |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Durham University, University of California - Santa Cruz, University of Maine |
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| Supplementary information: | Acquisition Description: Sampling Sites and Sample Collection: Tissue samples from Ross, Weddell, and crabeater seals were collected along western Antarctica from the West Antarctic Peninsula (WAP) to the Ross Sea during multiple field seasons and, in most cases, body mass, age class (juvenile, subadult, and adult), gender, and location were recorded for each sampled seal. Seals were sampled during the austral summers of 2008/09 and 2010/11 on RV Oden cruises along the western Antarctic coast. Mostly whole blood samples were obtained. In some cases, clot (blood with serum removed), red blood cells (RBCs, whole blood exposed to an anticoagulant, heparin, before having plasma removed), and hair samples (body fur or whiskers) were also taken. The sampling protocol is described in Aubail et al. (2011); all animal captures were conducted in accordance with the regulations of the Swedish Polar Research Secretariat (Registration No. 2010-112). All other samples were obtained from animal captures conducted under National Marine Fisheries Service permit No. 87-1851-00. Additionally, the Institutional Animal Care and Use Committee (IACUC) at the University of Santa Cruz (UC Santa Cruz) approved all protocols for the following samples. Whiskers were taken from crabeater seals during multiple cruises on the RV Lawrence M. Gould along the WAP. Plasma was also obtained from a few of the fall 2007 individuals (G105, G110, and G112). In addition, serum or plasma was obtained from two Weddell seals during the fall 2007 sampling in this region, and whiskers were taken from two WAP Weddell seals in the austral summer of 2009/10. Hückstädt et al. (2012b) describe the procedure for sampling the whiskers, and Goetz et al. (2017) describe the protocol used for collecting the seal serum and plasma. Several blood samples were obtained from Weddell seals in the McMurdo Sound region, Ross Sea, Antarctica over multiple field seasons. Twelve whole blood samples were taken from juvenile Weddell seals near Inexpressible Island (74.9 °S, 163.7 °E) during the austral summer of 2010/11. Whole blood samples were taken from Weddell seals in the austral summer of 2010/11 and austral spring of 2012. RBCs were sampled in the austral summer of 2009/10, austral summer of 2011/12, and austral spring of 2012. Whole blood, plasma, and serum were obtained from five Weddell seals sampled in the austral spring of 2015, and whole blood from an additional seven Weddell seals was also acquired during this time. Goetz et al. (2017) describe the sampling protocol for these Weddell seals. Lastly, a few samples were obtained from crabeater seals in McMurdo Sound. Hair samples were taken from three recently deceased juvenile crabeater seals that were found on the seasonal pack ice around Cape Royds in the austral summer of 2009/10. Whole blood was sampled, using the protocol of Goetz et al. (2017), from a male adult crabeater seal found in Erebus Bay during the austral summer of 2010/11. Taxonomic Groups: Pinnipedia Phocidae Lobodon carcinophaga - crabeater seal Ommatophoca rossii - Ross seal Leptonychotes weddellii - Weddell seal Sample Preparation: After sample collection, all samples were kept frozen at -20 °C. Blood samples were freeze-dried with a Labconco Freeze Dry System (Lyph Lock 4.5) and homogenized manually prior to analysis. Lipid extraction was not performed on the blood samples. Blood has a relatively low lipid content and a test set of blood samples with and without lipid extraction revealed no significant effect of lipid extraction on blood values. Hair samples, which have higher lipid contents, were lipid extracted. Hair samples were washed with Milli-Q water (Thermo Fisher Scientific, Inc.) and then rinsed 3 times in an ultrasonic bath with petroleum ether for 15 minutes. Hückstädt et al. (2012a) used a similar protocol to lipid-extract the crabeater seal whisker samples. Compound Specific Isotope Analysis (CSIA): CSIA was performed at UC Santa Cruz via gas chromatography-isotope ratio mass spectrometry (GC-IRMS). All samples were prepared for GC-IRMS analysis using the method described in McCarthy et al. (2007) and McCarthy et al. (2013). In brief, samples were hydrolyzed (6 N HCl for 20 hr at 110 °C) and converted to trifluoroacetic anhydride (TFAA) derivatives. Samples were stored in a -20 °C freezer in a 1:3 TFAA:DCM (methylene chloride) mixture until the day of instrumental analysis. Immediately before the analysis, the TFAA:DCM mixture was evaporated under N 2 and samples were diluted in ethyl acetate. Amino acid δ 15 N values were measured on a Thermo Trace GC coupled to a Thermo-Finnigan Delta Plus XP isotope-ratio-monitoring mass spectrometer (oxidation furnace at 980 ºC and reduction furnace at 650 ºC). The column for the N isotope analysis was a SGE Analytical Science BPX5 column 60 m by 0.32 mm with a 1 µm film thickness. The injector temperature was 250 ºC with a split He flow of 2 mL/min. The GC temperature program for δ 15 N analysis was: initial temp = 70 ºC hold for 1 min; ramp 1 = 10 ºC /min to 185 ºC, hold for 2 min; ramp 2 = 2 ºC/min to 200 ºC, hold for 10 min; ramp 3 = 30 ºC/min to 300 ºC, hold for 6 min. Directly measured amino acid δ 15 N values were corrected based on bracketing external standards, as described in McCarthy et al. (2013). Samples were injected and analyzed 3 to 4 times, and the mean value, standard deviation, and number of injections are reported. The δ 15 N values of 11 amino acids could be quantified. These were alanine (Ala), glycine (Gly), threonine (Thr), serine (Ser), valine (Val), leucine (Leu), Pro, aspartic acid + asparagine (Asp), Glu, Phe, and lysine (Lys). In all samples, isoleucine (Ile) was either not detectable or had low peak areas (70) when it was detectable. |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-04-20 20:10:05+00 |
| Editdate: | 2024-04-20 20:10:58+00 |