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OAS accession Detail for 0278402
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| accessions_id: | 0278402 | archive |
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| Title: | Water column amoA and Nitrospina-like 16S rRNA gene abundances from qPCR in the Eastern Tropical South Pacific using seawater collected on R/V Atlantis (AT15-61) cruise in Jan-Feb 2010 and R/V Melville (MV1104) cruise in Mar-Apr 2011 (NCEI Accession 0278402) |
| Abstract: | This dataset contains biological and survey - biological data collected on R/V Atlantis and R/V Melville during cruises AT15-61 and MV1104 in the South Pacific Ocean from 2010-02-02 to 2011-04-20. These data include abundance and depth. The instruments used to collect these data include CTD Sea-Bird 9, Niskin bottle, and qPCR Thermal Cycler. These data were collected by Karen L. Casciotti of Stanford University as part of the "Expression of Microbial Nitrification in the Stable Isotopic Systematics of Oceanic Nitrite and Nitrate (Microbial Nitrification)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-05-07. The following is the text of the dataset description provided by BCO-DMO: Water column amoA and Nitrospina-like 16S rRNA gene abundances from qPCR in the Eastern Tropical South Pacific Dataset Description: These data appear in the following: Figure S7 of Santoro, et al. (2020) Table S5 of Santoro, et al. (2020) Figure S6 in pre-print on ESSOAr ( doi.org/10.1002/essoar.10503499.1 ) |
| Date received: | 20210507 |
| Start date: | 20100202 |
| End date: | 20110420 |
| Seanames: | South Pacific Ocean |
| West boundary: | -100 |
| East boundary: | -80 |
| North boundary: | -9.943 |
| South boundary: | -20.01 |
| Observation types: | biological, survey - biological |
| Instrument types: | CTD, Niskin bottle, PCR machine |
| Datatypes: | DEPTH - OBSERVATION, species abundance |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Stanford University |
| Contributing projects: | |
| Platforms: | Atlantis (33AT), Melville (318M) |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Seawater samples were obtained during the R/V Atlantis (AT15-61) and R/V Melville (MV1104) cruises in Jan-Feb 2010 and Mar-Apr 2011. Water samples were collected at discrete depths using a standard 24-bottle Niskin rosette sampler equipped with an SBE9plus conductivity-temperature-depth (CTD) sensor package (SeaBird Electronics, Bellevue, WA). Samples for nucleic acid extraction and qPCR were collected from the rosette in 2-4 L polycarbonate bottles. Cells were harvested by pressure filtration onto 25 mm diameter, 0.2 µm pore-size polyethersulfone membrane filters (Supor-200, Pall Corporation, Port Washington, NY) housed in polypropylene filter holders (Whatman SwinLok, GE Healthcare, Pittsburgh, PA) using a peristaltic pump and silicone tubing. DNA was extracted according to Santoro,et al. (2010). For DNA extraction and analysis methods followed Santoro, et al. (2010). Sample volumes of 1-4 liters were filtered depending on the biomass present at each station and depth, and the filters were flash frozen in liquid nitrogen in 2 mL gasketed bead beating tubes (Fisher Scientific). |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-18 04:30:12+00 |
| Editdate: | 2023-05-18 04:31:06+00 |