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OAS accession Detail for 0278305
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| accessions_id: | 0278305 | archive |
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| Title: | Metagenomic data from samples collected on cruise Chikyu-331 in the Okinawa Trough, Japan from September to October 2010 (NCEI Accession 0278305) |
| Abstract: | This dataset contains physical data collected on Chikyu during cruise Chikyu-331 in the East China Sea (Tung Hai) from 2010-09-16 to 2010-09-27. These data include water temperature. The instruments used to collect these data include Automated DNA Sequencer and Piston Corer. These data were collected by Christopher House and Leah Brandt of Pennsylvania State University as part of the "An In-Depth analysis of the subvent biosphere within Okinawa Backarc Basin (IODP 331, Iheya North Hydrothermal Field) sediments (Subvent_Biosphere_Sediments)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" and "International Ocean Discovery Program (IODP)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-11-22. The following is the text of the dataset description provided by BCO-DMO: Metagenomic data Dataset Description: Next-generation sequencing data across a hydrothermal gradient; Okinawa Backarc Basin IODP 331 – subvent biosphere. The purpose these metagenomic data was to capture a distinct changeover of microbial assemblages between the mesophilic horizons above 10 mbsf and the hottest (55C) hydrothermal clay horizon. In this section of the dynamic Iheya North Hydrothermal system, the temperature gradient was estimated to be ~3C/m. These metagenomes provide functional evidence to this model, demonstrating that molecular signals represent a responsive microbial community to the increasingly demanding environmental conditions. |
| Date received: | 20191122 |
| Start date: | 20100916 |
| End date: | 20100927 |
| Seanames: | East China Sea (Tung Hai) |
| West boundary: | 126.901 |
| East boundary: | 126.901 |
| North boundary: | 27.79 |
| South boundary: | 27.79 |
| Observation types: | physical |
| Instrument types: | corer |
| Datatypes: | WATER TEMPERATURE |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | The Pennsylvania State University |
| Contributing projects: | |
| Platforms: | Chikyu (49Y9) |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Sampling of cores was done in accordance with IODP protocols. All C0014 samples were cored on 16 September 2010 via via HPCS (hydraulic piston coring system). All C0015 samples were cored on 18 September 2010 via HPCS. All C0017 samples were cored on 27 September 2010 via HPCS. Core sections were kept frozen (-80°C) until DNA extractions. A MoBio® Power Soil kit was used to extract environmental DNA. Metagenomic data was produced from whole genome amplified environmental DNA (REPLI-g Mini Kit, Qiagen). Sequencing was performed at the Penn State Genomics Core Facility – University Park, PA using the Illumina® HiSeq 2500 (NSF-MRI award DBI-1229046 (Axtell et al., 2012)). The sequencing facility prepared DNA libraries using Nextera XT Library Preparation Kits prior to sequencing. Together, the samples were run on one-half of a sequencing plate. This sequencing run was run using rapid-run model that averages up to 300 million single reads, or 600 million paired reads of ~150 nucleotides per rapid-run. |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-16 04:51:41+00 |
| Editdate: | 2023-05-16 04:52:28+00 |