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OAS accession Detail for 0277894
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| accessions_id: | 0277894 | archive |
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| Title: | Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project) (NCEI Accession 0277894) |
| Abstract: | This dataset contains chemical and physical data collected on R/V Atlantis during cruise AT26-10 from 2014-01-03 to 2014-01-20. These data include Ammonium, Nitrate, pH, water pressure, and water temperature. The instruments used to collect these data include Isobaric Gas-Tight Sampler, Microscope-Electron, Microscope-Optical, and Shipboard Incubator. These data were collected by Dr Dionysis Foustoukos of Carnegie Institution for Science as part of the "An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents (Microbial Communities at Deep-Sea Vents)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" and "Dimensions of Biodiversity (Dimensions of Biodiversity)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2017-02-13. The following is the text of the dataset description provided by BCO-DMO: Results of on-board incubations of microbes in diffuse flow vent fluids collected from Crab Spa and Alvinella patch Dataset Description: This dataset includes results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa (9.8398º N, 104.2913º W) and Alvinella (9.8398º N, 104.2915º W) sites at East Pacific Rise during the AT26-10 oceanographic expedition in January 2014. Reported parameters include dates and time elapsed, flow rate, temperature, pressure, and pH, and concentrations of NO3, NH4, H2, H2S, CH4. Vent fluids used in onboard incubations were corrected from diffuse flow vent sites at the East Pacific Rise (2503 m): Crab Spa (9.8398º N, 104.2913º W) and Alvinella (9.8398º N, 104.2915º W) (see description in McNichol et al. [2016]). Fluids were collected using isobaric gas-tight samplers [Seewald et al., 2002] prior to their transfer to the shipboard continuous culture system [Foustoukos and Perez-Rodriguez, 2015]. Here, high-pressure incubations (250 bars) were conducted at mesophilic (30 ºC) and thermophilic (50 ºC) conditions to constrain the function and metabolic rates of denitrifying and DNRA microbial communities residing at Crab Spa and Alvinella, respectively. To enhance the activity of nitrate-respiring anaerobic bacteria, an NO3- (5 mm) and H2(aq) (1.30 mM)-enriched medium was introduced in the high-pressure incubations under strictly anaerobic conditions. Dissolved HCO3- (7.3 mm, 13C labeled) was used as added carbon source. Vent fluids were introduced at a flow rate of 0.042 mL/min, while growth medium was added at a rate of 0.0042 mL/min. The two sets of experiments took place for 356 (Crab Spa) and 50 hours (Alvinella). Direct cell counts were conducted by staining cells with 0.1% acridine orange and counting them with a fluorescence microscope. 15N/14N isotopic analysis of the NO3-, NH4+ and biomass were conducted with a Thermo Scientific Delta VPlus mass spectrometer and CE Instruments NA 2500 series elemental analyzer (EA). References: Foustoukos, D., and I. Perez-Rodriguez (2015), A continuous culture system for assessing microbial activities in the piezosphere, Applied and Environmental Microbiology, 81(19), 6850-6856. McNichol, J., S. P. Sylva, F. Thomas, C. D. Taylor, S. M. Sievert, and J. S. Seewald (2016), Assessing microbial processes in deep-sea hydrothermal systems by incubation at in situ temperature and pressure, Deep Sea Research Part I: Oceanographic Research Papers, 115, 221-232. Seewald, J. S., K. W. Doherty, T. R. Hammar, and S. P. Liberatore (2002), A new gas-tight isobaric sampler for hydrothermal fluids, Deep-Sea Research, Part I: Oceanographic Research Papers, 49(1), 189-196. |
| Date received: | 20170213 |
| Start date: | 20140103 |
| End date: | 20140120 |
| Seanames: | |
| West boundary: | -104.2915 |
| East boundary: | -104.2915 |
| North boundary: | 9.83992 |
| South boundary: | 9.83992 |
| Observation types: | chemical, physical |
| Instrument types: | microscope |
| Datatypes: | AMMONIUM (NH4), HYDROSTATIC PRESSURE, NITRATE, pH, WATER TEMPERATURE |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | |
| Contributing projects: | |
| Platforms: | Atlantis (33AT) |
| Number of observations: | |
| Supplementary information: | Acquisition Description: From AT26-10 cruise report (01/29/2014): DOB: An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents Cruise Report by the CIW research team: Dr. Ileana Perez-Rodriguez, Mr. Matt Rawls and Dr. Dionysis I. Foustoukos The CIW team was responsible for the shipboard continuous culturing incubations of vent fluids collected from Crab Spa and Tica hot springs during the AT26-10 expedition at 9oN EPR by utilizing our high-pressure bioreactor (Fig. 1). This was accomplished through a collaborative effort with Jeff Seewald and Sean Sylva (WHOI), who deployed isobaric gas-tight samplers (IGTs) to collect hydrothermal vent fluids at the diffuse flow sites. Experiments were designed to study the cycling to N through the metabolic processes of denitrification and dissimilatory nitrate reduction to ammonia (DNRA) under in-situ deep-sea vent temperature and pressure conditions. We studied the evolution of nitrate reducing microorganisms at mesophilic (30oC) and thermophilic (50oC) conditions at pressures ranging from 5 to 250 bar. Vent fluids (16 IGTs) were delivered in the bioreactor and homogeneously mixed with aqueous media solution enriched in dissolved nitrate, hydrogen and 13C labeled bicarbonate to facilitate the growth of nitrate reducing microorganisms (Fig. 2). The two distinct sets of experiments were lasted for 356 and 100 hours. In short, experimental results constrained the function and metabolic rates of the denitrifying microbial communities in the Crab Spa fluids, while DNRA metabolic pathways were identified for the populations residing in the moderate temperature vent fluids (60oC) of the Alvinella colony at Tica. During the course of the experiments we monitored the growth of deep-sea microbial communities by measuring the concentrations of dissolved aqueous species directly involved in nitrate based metabolism, such as NO3, NH4, H2 and H2S. We also monitored cell densities by utilizing an epi-fluorescence microscope (Sievert, WHOI). Dissolved gas and NH4+ concentrations were attained by gas and ion chromatography (Seewald - Sylva, WHOI). Subsamples were also collected for a number of offshore analysis to determine: i) the 15N/14N isotope composition of NO3-,/NH4+ and constrain kinetic isotope effects associated with denitrification/DNRA (Perez-Rodriguez, CIW), ii) to study the rates of autotrophic carbon fixation by NanoSIMS (Musat, UFZ), iii) to perform single cell genomics on the microbial populations grown in the bioreactor (Ramunas, Bigelow) and (iv) to isolate and characterize novel microogranisms from the communities cultured in our experiments (Perez-Rodriguez, CIW and Vetriani, Rutgers). |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-05 04:17:09+00 |
| Editdate: | 2023-05-05 04:17:40+00 |