The Ocean Archive System searches our original datasets as they were submitted to us, not individual points or profiles. If you want to search and retrieve ocean profiles in a common format, or objectively analyzed fields, your better option may be to use one of our project applications. See: Access Data
OAS accession Detail for 0294411
| << previous | |revision: 1 |
| accessions_id: | 0294411 | archive |
|---|---|
| Title: | Oyster larvae vertical distribution data collected from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017 (NCEI Accession 0294411) |
| Abstract: | This dataset contains biological and survey - biological data collectedat Coastal Pacific, USA from 2017-07-21 to 2017-08-03. These data include depth and relative abundance. The instruments used to collect these data include Camera and In-situ incubator. These data were collected by Dr Brady M. Olson, Dr Sylvia Yang, and Shawn M Arellano of Western Washington University as part of the "RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal (Climate stressors on larvae)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-09-25. The following is the text of the dataset description provided by BCO-DMO: Ostrea_Behavior_OA_Expt2017 Dataset Description: Oyster larvae vertical distribution data collected from a laboratory water column experiments to investigate the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017. |
| Date received: | 20190925 |
| Start date: | 20170721 |
| End date: | 20170803 |
| Seanames: | |
| West boundary: | |
| East boundary: | |
| North boundary: | |
| South boundary: | |
| Observation types: | biological, survey - biological |
| Instrument types: | camera |
| Datatypes: | species abundance |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Western Washington University |
| Contributing projects: | |
| Platforms: | |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Collection & Larval Rearing We collected adult Olympia oysters ( Ostrea lurida ) from Fidalgo Bay in June 2017 and maintained them in a sea table with continuous flowing seawater heated to 19-20°C at the Shannon Point Marine Center. We fed adult oysters were fed concentrated algae once a day (Shellfish Diet, Reed Mariculture) and utilized banjo-style filters (60-m) attached to the outflow pipes of the sea table to catch released O. lurida larvae. We then collected and reared larvae at 12°C in 3-L jars (2 individuals mL-1). Each jar of larvae received a 50% water change with 0.35-m filtered sea water and were fed Isochrysis galbana algae (50,000 cells mL-1) daily. Experimental Design To measure the effect of pH conditions on the vertical distribution of larvae we established three experimental pycnocline treatments within clear plexiglass water columns (2.5cm x 2.5cm x 30cm): (1) ambient water (400ppm) in the top layer and acidic water in the bottom layer (1500ppm), (2) ambient water (400ppm) in both top and bottom layers, and (3) acidic water (1500ppm) in the top layer and ambient water (400ppm) in the bottom layer. Each water layer was 60-mL of water and filled the column 10-cm high, so when each experimental treatment was established it filled the column to 20-cm. We established the experimental treatments by increasing the density of seawater in the bottom layer by 0.003-0.005 g ml-1 using PercollTM GE Healthcare (Podolsky & Emlet 1993). Experimental treatment water was kept at 12°C and pre-equilibrated to the desired pCO2 level and density. We also included blue food coloring (1 drop per 100-mL) to the dense bottom layer to more easily visualize the density layers while establishing experimental treatments. We set-up four replicate columns for each experimental treatment making twelve columns total per experiment. On the day of each experiment, we incubated the experimental treatment columns in clear plexiglass water baths connected to a Fisher Scientific Isotemp recirculating water bath to maintain treatment temperature at 12°C throughout the experiment. We carefully injected 150 larvae by syringe into the bottom 2-cm of each column with no more than 2-mL of their culture water. Olympia oyster larvae are highly phototactic (personal observations), so larvae were kept in the dark and we video recorded their vertical positions under infrared light two times: the first time at 10 minutes of acclimation in the columns and the second time at 30 minutes of acclimation in the columns. To record, we used an infrared uEye camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand. We later counted by eye the number of larvae per centimeter area of each column from the videos. |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-06-27 21:00:50+00 |
| Editdate: | 2024-06-27 21:01:21+00 |