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OAS accession Detail for 0294406
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| accessions_id: | 0294406 | archive |
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| Title: | Cluster (combined temperature, nutrient concentration, and CO2) results from laboratory experiments with Pseudo-nitzschia australis conducted from 2021 to 2022 (NCEI Accession 0294406) |
| Abstract: | This dataset contains biological and chemical data collected from 2020-11-01 to 2022-07-01. These data include Partial pressure of CO2, dissolved inorganic Carbon, domoic acid, growth, pH, primary production, and total alkalinity (TA). The instruments used to collect these data include Elemental Analyzer, Fluorometer, High-Performance Liquid Chromatograph, Liquid Scintillation Counter, Mass Spectrometer, Microscope - Optical, Spectrometer, and pH Sensor. These data were collected by Andrew Kim, Bethany D. Jenkins, and Matthew Bertin of University of Rhode Island and Amjad Mansour, David A. Hutchins, Feixue Fu, Kyla Jean Kelly, Liang Chen, and Lily A Mancini of University of Southern California as part of the "MCA: Developing transcriptomics as a tool to investigate toxic diatom responses to ocean heatwave and upwelling events (Toxic diatoms and heatwaves)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-11-15. The following is the text of the dataset description provided by BCO-DMO: Methods and Sampling: These experiments were conducted with a strain of (strain NWFSC 731) was isolated from Long Beach, Washington State, USA on November 3, 2020. The temperature and salinity were 14°C and 27 ppt, respectively at the time of collection. The data was collected in laboratory experiments at the University of Southern California. The experiments began in September 2021 and finished in July of 2022. These experiments were a cluster of combined temperature, nutrient concentration, and CO2 in order to reflect upwelling, heatwaves, and extreme heatwaves in the natural environment. LTCN was a treatment to test the interactive effects. Upwelling: 13°C, high CO2, high nutrients Heatwave: 19°C, low CO2, low nutrients Extreme heatwave: 21°C, low CO2, low nutrients LTCN (low temperature, CO2, and nitrogen): 13°C, low CO2, low nutrients The following section provides a methodology summary for this dataset and references related datasets collected as part of the same experiment (see "Related Datasets" section for data access). A full methodology was published in "Simulated upwelling and marine heatwave events promote similar growth rates but differential domoic acid toxicity in Pseudo-nitzschia australis" in Harmful Algae (Kelly et al., 2023). Pseudo-nitzschia australis was grown under upwelling heatwave, and extreme heatwave conditions (e.g., combined temperature, nutrient, and carbon dioxide levels specific to each condition) and in single-factor response curves for carbon dioxide, temperature, and varying nitrogen:phosphorus (N:P) ratios/total nutrient concentrations. Samples for chlorophyll a (used to calculate growth rates) were filtered on GF/F filters, extracted in 6 mL of 90 % acetone at -20°C for 24 h, then analyzed using a Turner 10AU field fluorometer (Welschmeyer 1994; Fu et al. 2007). For elemental analysis (particulate organic carbon and nitrogen, POC and PON), cells were filtered onto pre-combusted GF/F filters, dried, and analyzed on a Costech 4010 Elemental Analyzer (Fu et al. 2007). Samples for particulate domoic acid were filtered onto Supor 0.2 µm 47 mm PES filters. Samples were analyzed using LC-MS/MS on a Prominence UFLC system (Shimadzu, Kyoto, Japan) coupled to a SCIEX 4500 QTRAP mass spectrometer (AB Sciex, Framingham, MA, USA). Methods described in Wang et al. 2012. Primary production was determined by measuring the uptake of radiolabeled bicarbonate (Fu et al. 2008). 14C-bicarbonate was added to 45 mL sub-cultures at T24 h and incubated for 24 h (approximating net carbon fixation) under the respective experimental conditions. After the incubation period, cells were collected on GF/F filters and placed in a scintillation vial containing scintillation cocktail. Samples were stored for 24 h before being read on a Wallac System 1400 liquid scintillation counter. pH measurements were made on a Mettler Toledo SevenCompact pH meter using a three-point calibration curve and total pH scale (Cooley and Yager 2006). Samples for total DIC analysis were collected at Tfinal. Seawater from undisturbed culture bottles was removed with a sterile syringe, ejected into pre-evacuated borosilicate Exetainers, and poisoned with 5% MgCl2. Total DIC was then measured using a Picarro cavity ring-down spectrophotometer according to Subhas et al. (2015). For cell count samples (for normalizing cellular domoic acid), 1 mL of the final experimental culture was preserved with 40 ul glutaraldehyde and stored at 4°C in the dark. Cells were counted on a Olympus BX51 microscope using a Sedgewick Rafter Chamber. Organism: Pseudo-nitzschia australis, LSID (urn:lsid:marinespecies.org:taxname:246604) |
| Date received: | 20231115 |
| Start date: | 20201101 |
| End date: | 20220701 |
| Seanames: | |
| West boundary: | -124.060591 |
| East boundary: | -124.060591 |
| North boundary: | 46.495103 |
| South boundary: | 46.495103 |
| Observation types: | biological, chemical |
| Instrument types: | chromatograph, fluorometer, mass spectrometer, microscope, pH sensor, scintillation counter |
| Datatypes: | DISSOLVED INORGANIC CARBON (DIC), growth rate, partial pressure of carbon dioxide - water, pH, PRIMARY PRODUCTIVITY, total alkalinity, TOXIN - DOMOIC ACID |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | The University of Rhode Island, University of Southern California |
| Contributing projects: | |
| Platforms: | |
| Number of observations: | |
| Supplementary information: | |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-06-27 20:53:29+00 |
| Editdate: | 2024-06-27 20:54:02+00 |