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OAS accession Detail for 0294403
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| accessions_id: | 0294403 | archive |
|---|---|
| Title: | Microbial enzyme activities: polysaccharide hydrolase activities in bulk seawater samples from the RV\Sonne cruise SO248 in the South and North Pacific, along 180 W, May, 2016 (NCEI Accession 0294403) |
| Abstract: | This dataset contains data collected on R/V Sonne during cruise So248 in the Bering Sea, North Pacific Ocean, and South Pacific Ocean from 2016-05-02 to 2016-05-29. These data include depth. The instruments used to collect these data include CTD profiler, Fluorometer, Gel Permeation Chromatograph, and Niskin bottle. These data were collected by Carol Arnosti of University of North Carolina at Chapel Hill as part of the "Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? (Patterns of activities)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-04-27. The following is the text of the dataset description provided by BCO-DMO: SO248: Bulk FLA Dataset Description: This dataset includes polysaccharide hydrolysis rates measured in bulk (not filter-fractionated) seawater. Samples were collected on RV/Sonne cruise SO248 in May 2016. Links to archived CTD data are also provided. |
| Date received: | 20200427 |
| Start date: | 20160502 |
| End date: | 20160529 |
| Seanames: | Bering Sea, North Pacific Ocean, South Pacific Ocean |
| West boundary: | 176.999 |
| East boundary: | -176.475 |
| North boundary: | 57 |
| South boundary: | -30.001 |
| Observation types: | |
| Instrument types: | chromatograph, CTD, fluorometer, Niskin bottle |
| Datatypes: | DEPTH - OBSERVATION |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | University of North Carolina - Chapel Hill |
| Contributing projects: | |
| Platforms: | SONNE (06BE) |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD. The potential of the seawater microbial community to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan) was investigated in surface and bottom water. For each substrate, three 50 mL falcon tubes were filled with seawater and one 50 mL falcon tube was filled with autoclaved seawater to serve as a killed control. Substrate was added at 3.5 uM monomer-equivalent concentrations, except for fucoidan, which was added at 5 uM concentrations (a higher concentration was necessary for sufficient fluorescence signal). Two 50 mL falcon tubes – one with seawater and one with autoclaved seawater – with no added substrate served as blank controls. Incubations were stored in the dark at as close to in situ temperature as possible. Subsamples of the incubations were collected at time zero, and at six subsequent timepoints (t1-t6): 2 days, 5 days, 10 days, 17 days, 30 days, and 42 days. At each timepoint, 2 mL of seawater was collected from the 50 mL falcon tube using a sterile syringe, filtered through a 0.2 um pore size syringe filter, and stored frozen until processing. The hydrolysis of high molecular weight substrate to lower molecular weight hydrolysis products was measured using gel permeation chromatography with fluorescence detection, after the method of Arnosti [1996, 2003]. In short, the subsample was injected onto a series of columns consisting of a 21 cm column of G50 and a 19 cm column of G75 Sephadex gel. The fluorescence of the column effluent was measured at excitation and emission wavelengths of 490 and 530 nm, respectively. Hydrolysis rates were calculated from the change in molecular weight distribution of the substrate over time, as described in detail in Arnosti [2003]. ara = arabinogalactan chn = chondroitin sulfate fuc = fucoidan lam = laminarin pul = pullulan xyl = xylan |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-06-27 20:48:54+00 |
| Editdate: | 2024-06-27 20:49:45+00 |