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OAS accession Detail for 0292220
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| accessions_id: | 0292220 | archive |
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| Title: | Metabarcoding samples collected from surface and chlorophyll maximum depths from R/V Pt. Sur PS 18-09 Legs 01 and 03, Hurricane Harvey RAPID Response cruise (western Gulf of Mexico) September-October 2017 (NCEI Accession 0292220) |
| Abstract: | This dataset contains biological data collected on R/V Point Sur during cruise PS1809 from 2017-09-23 to 2017-10-01. These data include taxon. The instruments used to collect these data include Automated DNA Sequencer, Niskin bottle, and Spectrophotometer. These data were collected by Darren W. Henrichs and Lisa Campbell of Texas A&M University as part of the "RAPID: Hurricane Impact on Phytoplankton Community Dynamics and Metabolic Response (HRR)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-02-10. The following is the text of the dataset description provided by BCO-DMO: HRR metatranscriptome Dataset Description: Metabarcode samples collected from surface and chlorophyll maximum depths from R/V Pt. Sur PS 18-09 Legs 01 and 03, Hurricane Harvey RAPID Response cruise (western Gulf of Mexico) September-October 2017. |
| Date received: | 20210210 |
| Start date: | 20170923 |
| End date: | 20171001 |
| Seanames: | |
| West boundary: | -97.268 |
| East boundary: | -94.9 |
| North boundary: | 29.0649 |
| South boundary: | 27.2286 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: On each of 2 cruise legs 01 and 03, samples were collected at 7 stations (S01, S06, S11, S16, S21, SS and GI) from 2 depths [surface and chlorophyll maximum depth when possible; see HRR-bottle data] ) and triplicate 500-1000 ml samples were filtered and immediately fixed in RNALater. Triplicate samples from each station/depth were extracted with AllPrep DNA/RNA MiniKit (Qiagen, USA) following the manufacturer’s instructions. DNA concentration and quality were evaluated using a Nanodrop spectrophotometer (Thermo Fisher Scientific Inc, USA). All the samples extracted for DNA were normalized to 5ng/µl concentration for the amplicon library construction. The V4 regions of the 18S rRNA genes were amplified using customized V4 primers (Bradley et al. 2016; Kozich et al. 2013). Library construction and amplicon sequencing was performed at Texas A&M University Agrilife’s Genomics and Bioinformatics Services (https://www.txgen.tamu.edu) using custom designed primers (Bradley et al. 2016; Kozich et al. 2013). Output of MiSeq results as fasta files were deposited in GenBank under the project number PRJNA592369. Leg 01 Station 01 sample was not collected. Sampling locations: Sample ID Station Leg Location Lat o N/Long o W na 1 27.2286 -97.2686 L3_S01 S01 3 L1_S06 S06 1 27.8358 -96.9874 L3_S06 S06 3 L1_S11 S11 1 28.2614 -96.4129 L3_S11 S11 3 L1_S16 S16 1 28.5366 -95.8656 L3_S16 S16 3 L1_S21 S21 1 28.7644 -95.2978 L3_S21 S21 3 L1_SS SS 1 28.9600 -95.0946 L3_SS SS 3 L1_GI GI 1 29.0649 -94.9000 L3_GI GI 3 |
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| Metadata version: | 1 |
| Keydate: | 2024-05-02 13:15:11+00 |
| Editdate: | 2024-05-02 13:16:09+00 |