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OAS accession Detail for 0292202
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| accessions_id: | 0292202 | archive |
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| Title: | Diel proteomes of cultured Trichodesmium erythraeum sp. IMS101 from laboratory experiments conducted in November of 2018 (NCEI Accession 0292202) |
| Abstract: | This dataset contains biological and chemical data collected on 2018-11-01. These data include proteins. The instruments used to collect these data include Mass Spectrometer. These data were collected by Mak A. Saito of Woods Hole Oceanographic Institution as part of the "Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)" and "New technology for high resolution analysis of proteins and other organic materials produced by marine microorganisms (MM Proteins and Organics Tech)" projects. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-03-25. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Diel proteomes of cultured Trichodesmium erythraeum sp. IMS101 from laboratory experiments conducted in November of 2018. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [1] partner repository with the dataset identifier PXD016332 and 10.6019/PXD016332 but are not yet public. Project Name: Trichodesmium erythraeum sp. IMS101 Diel proteomes Project accession: PXD016332 Project https://doi.org/10.6019/PXD016332 The format of these data in the BCO-DMO data system is tabular. For a version formatted as a matrix, see the "Data Files" section. |
| Date received: | 20200325 |
| Start date: | 20181101 |
| End date: | 20181101 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: A batch culture (1.5L) of Trichodesmium erythraeum sp. IMS101 was grown in a 27°C incubator with a 14:10 light cycle that ramps up and down mimicking dawn and dusk. Sampling occurred every 1-3 hours with concentrated sampling at dawn and dusk. 70mL of the culture was sterically subsampled and collected on 0.2mm Supor filters, then frozen at -80°C. 10mL was collected and filtered on combusted GFF filters for CHN analysis. Proteins were extracted in sodium dodecyl sulfate and digested in gel similar to Saito et al., 2014 (Science). Peptides were analyzed by LC-MS/MS on a Thermo Orbitrap Fusion using LC x LC/MS chromatography with high and low pH reversed phase chromatography. |
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| Metadata version: | 1 |
| Keydate: | 2024-05-02 12:49:07+00 |
| Editdate: | 2024-05-02 12:49:50+00 |