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OAS accession Detail for 0292196
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| accessions_id: | 0292196 | archive |
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| Title: | Growth rates of the polar diatom Chaetoceros RS19 under various +Zn and +Co conditions from September 2019 (MM Saito project) (NCEI Accession 0292196) |
| Abstract: | This dataset contains biological, chemical, and physical data collected from 2019-09-01 to 2019-09-30. These data include Cobalt, Zinc, growth, and trace metal concentration. The instruments used to collect these data include In-situ incubator and Turner Designs 700 Laboratory Fluorometer. These data were collected by Mak A. Saito of Woods Hole Oceanographic Institution as part of the "Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)" project and "Marine Microbiology Initiative (MMI)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-09-20. The following is the text of the dataset description provided by BCO-DMO: Growth rates of the Ross Sea diatom isolate Chaetoceros sp. RS19 under various Zinc and Cobalt additions. Dataset Description: Growth rates of the Ross Sea diatom isolate Chaetoceros sp. RS19 under various Zn and Co additions. |
| Date received: | 20210920 |
| Start date: | 20190901 |
| End date: | 20190930 |
| Seanames: | |
| West boundary: | 177.12379 |
| East boundary: | 177.12379 |
| North boundary: | -76.48338 |
| South boundary: | -76.48338 |
| Observation types: | biological, chemical, physical |
| Instrument types: | fluorometer |
| Datatypes: | growth rate, TOXIC METALS, TRACE METALS, zinc |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Woods Hole Oceanographic Institution |
| Contributing projects: | |
| Platforms: | |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Methodology: Sampling and analytical procedures: Media and culturing techniques Chaetoceros RS19 cultures were maintained in a 4°C incubator under constant fluorescent lighting (30 µmol photon m -2 s -1 ). All cultures were randomly repositioned each day to avoid any effect of subtle variation in light intensity on growth. Chaetoceros RS19 was originally isolated by D. Moran from the Ross Sea, Antarctica, and cultures were maintained in the Saito laboratory culture collection at the Woods Hole Oceanographic Institution. All cultures were axenic and maintained by sterile technique until needed. Polycarbonate and plastic bottles were cleaned to remove trace metal contaminants before use. This procedure involved, at minimum, a 72h soak in Culture media was prepared after that used by Sunda and Huntsman for trace metal experimentation (Sunda and Huntsman 1995). Microwave sterilized, 0.2 µm-filtered Equatorial Pacific surface seawater collected at station 14 of the 2016 ProteOMZ expedition (10.5600°S, 146.3133° W; cruise code FK160115) was used as the media base. Macronutrients were added to this sterile base to a final concentration of 88.2 µM NaNO 3 , 41.5 µM NaH 2 PO 4 , and 106 µM Na 2 SiO 3 and were chelexed before use. Added vitamins included 2 nM biotin, 0.37 nM B 12 as cyanocobalamin, and 300 nM thiamine and were also chelexed before use. Trace metals were added to final media concentrations of 10 -7 M FeCl 3 , 4.8 x 10 -8 M MnCl 2 , 4.0 x 10 -8 M CuSO 4 , 10 -7 M NiCl 2 , and 10 -8 M Na 2 O 3 Se within a 10 -4 M ethylenediamine tetraacetic acid disodium salt (EDTA, Acros Organics, C 10 H 14 N 2 Na 2 O 8 ) metal ion buffer system. All media amendments were sterile filtered through acid-rinsed 0.2 µm filters before addition to final media, and final media equilibrated for at least 12h before inoculation. Established cultures were first acclimated in low-metal media containing 1 nM total added Zn or less for at least three transfers. These acclimated cultures were used to inoculate initial cultures at 1% volume. All growth media was chilled at 4°C prior to inoculation. Zn or Co limitation experiments were first performed using a range of added Zn concentrations with Co omitted and vice versa. We refer to growth rate experiments using media amended with Zn or Co (while omitting the other) as “simple limitation” experiments. Growth rate experiments in which one metal was held at a constant total added value while varying the added concentration of the other metal were also conducted—we refer to these as “double addition” experiments. Growth of all experiment cultures was monitored by relative chlorophyll fluorescence using a Turner TD-700 fluorometer, calibrated prior to measurement with a solid standard. All cultures were grown in 28 mL acid cleaned, microwave sterilized polycarbonate tubes (Nalgene) that are compatible with the fluorometer, which enabled measurements to be taken without exposing cultures to contaminating metals. Growth rates were calculated as the slope of the natural logarithm of the increase in chlorophyll fluorescence over a four-measurement (usually 4 day) period during exponential growth. Computed ratios of [Zn 2+ ] and [Co 2+ ] to total concentrations, whose values are 10 -3.99 and 10 -3.63 respectively, were used to convert total added metal concentrations to free ion concentrations and are the same as those used by Sunda and Huntsman (Sunda and Huntsman 1995). |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-05-02 12:39:57+00 |
| Editdate: | 2024-05-02 12:40:18+00 |