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OAS accession Detail for 0291947
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| accessions_id: | 0291947 | archive |
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| Title: | Coccolithophore-associated organic biopolymers for fractionating particle-reactive radionuclides (234Th, 233Pa, 210Pb, 210Po, and 7Be) on 2018-05-15 (NCEI Accession 0291947) |
| Abstract: | This dataset contains biological and chemical data collected on 2018-05-15. These data include carbohydrates and proteins. The instruments used to collect these data include Centrifuge, Gamma Ray Spectrometer, Liquid Scintillation Counter, and Spectrometer. These data were collected by Antonietta Quigg, Chen Xu, Kathleen Schwehr, and Peter Santschi of Texas A&M, Galveston as part of the "Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores (Biopolymers for radionuclides)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-03-28. The following is the text of the dataset description provided by BCO-DMO: Recoveries and partition coefficients of Po, Pb and Be Dataset Description: Laboratory incubation experiments using the coccolithophore Emiliania huxleyi were conducted in the presence of 234Th, 233Pa, 210Pb, 210Po, and 7Be to differentiate radionuclide uptake to the CaCO3 coccosphere from coccolithophore-associated biopolymers. |
| Date received: | 20190328 |
| Start date: | 20180515 |
| End date: | 20180515 |
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| Observation types: | biological, chemical |
| Instrument types: | mass spectrometer, scintillation counter |
| Datatypes: | BIOCHEMISTRY |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Texas A&M University - Galveston |
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| Supplementary information: | Acquisition Description: The seawater ( Non-attached exopolymeric substances (NAEPS) and exopolymeric substances attached on the coccolithophore cellular surface (AEPS) were extracted followed the procedures described in Chuang et al. (2015) and Xu et al. (2011). In brief, laboratory cultures were centrifuged at 3000 ×g for 30 min, and then the supernatant for the NAEPS fraction was filtered, followed by the concentration and extensive desalting of supernatant against nanopure water (18.2 Ω) with 3 kDa Microsep centrifugal filter tubes (Milipore). For AEPS extraction, the resultant pellet from the centrifugation was resuspended by 50 mL 3% NaCl solution and stirred gently overnight at 4ºC. Lastly, the solution was centrifuged, and the supernatant containing the AEPS was then filtered before further desalting via the 3 kDa ultrafiltration centrifugation tubes. The pellet from the previous step was thus further digested in the 0.44 M HAc + 0.1 M NaCl solution at 4ºC for 8 h. After the digestion, the mixed solution was centrifuged and filtered, followed by ultrafiltration of the supernatant with 3 kDa Microsep centrifugal filter tubes. The retentate (> 3 kDa) was defined as coccosphere-associated biopolymers. The permeate ( Subsamples were taken from the concentrated biopolymers for the analysis of protein, total carbohydrate (TCHO) and uronic acid (URA), respectively. In brief, the protein abundance was measured through a modified Lowry protein assay, using bovine serum albumin (BSA) as the standard. For the concentrations of TCHO, samples were hydrolyzed by 0.09 M HCl (final concentration) at 150ºC for 1 h. After neutralization with NaOH solution, the hydrolysate was measured by the 2,4,6-tripyridyl-triazine (TPTZ) method (Hung et al., 2001), with glucose as the standard. URA concentrations were determined by the metahydroxyphenyl method using glucuronic acid as the standard (Hung and Santschi, 2001). All the solutions from the different extraction steps, including the >3 kDa biopolymer fractions and the permeate ( |
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| Metadata version: | 1 |
| Keydate: | 2024-04-27 17:34:41+00 |
| Editdate: | 2024-04-27 17:35:00+00 |