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OAS accession Detail for 0291931
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| accessions_id: | 0291931 | archive |
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| Title: | Large substrate hydrolysis of bulk water samples taken aboard the R/V Endeavor EN638, May 2019 in the Northern Atlantic (NCEI Accession 0291931) |
| Abstract: | This dataset contains data collected on R/V Endeavor during cruise EN638 from 2019-05-14 to 2019-05-25. These data include depth. The instruments used to collect these data include High Performance Liquid Chromatograph. These data were collected by Carol Arnosti of University of North Carolina at Chapel Hill as part of the "A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation (Microbial DOC Degradation)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-09-24. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: The potential of the seawater microbial community to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan) was investigated in surface and bottom water. Seawater has been collected during the EN638 cruise on the R/V Endeavor in May 2019. |
| Date received: | 20200924 |
| Start date: | 20190514 |
| End date: | 20190525 |
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| West boundary: | -75.67819 |
| East boundary: | -53.3949 |
| North boundary: | 42.83954 |
| South boundary: | 34.50102 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD. Seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. From each carboy, water was dispensed into smaller glass containers that were cleaned and pre-rinsed three times with water from the carboy prior to dispensing. This water was used to measure cell counts, bacterial productivity, and the activities of polysaccharide hydrolases, peptidases, and glucosidases. A separate glass Duran bottle was filled with seawater from the carboy and sterilized in an autoclave for 20-30 minutes to serve as a killed control for microbial activity measurements. The potential of the seawater microbial community to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan) was investigated in surface and bottom water. For each substrate, three 50 mL falcon tubes were filled with seawater and one 50 mL falcon tube was filled with autoclaved seawater to serve as a killed control. Substrate was added at 3.5 μM monomer-equivalent concentrations, except for fucoidan, which was added at 5 μM concentrations (a higher concentration was necessary for sufficient fluorescence signal). Two 50 mL falcon tubes – one with seawater and one with autoclaved seawater – with no added substrate served as blank controls. Incubations were stored in the dark at as close to in situ temperature as possible. Subsamples of the incubations were collected at time zero, and at a sequence of subsequent time points. At each time point, 2 mL of seawater was collected from the 50 mL falcon tube using a sterile syringe, filtered through a 0.2 μm pore size syringe filter, and stored frozen until processing. |
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| Metadata version: | 1 |
| Keydate: | 2024-04-27 17:16:18+00 |
| Editdate: | 2024-04-27 17:16:34+00 |