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OAS accession Detail for 0291926
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| accessions_id: | 0291926 | archive |
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| Title: | Quantification of different chemical forms of cobalamin from San Pedro Ocean Time Series (SPOT) cruises on R/V Nerissa and R/V Yellowfin from March to December 2017 (NCEI Accession 0291926) |
| Abstract: | This dataset contains data collected on R/V Nerissa and R/V Yellowfin during cruises SPOT_Nerissa_Cruises_2017 and SPOT_Yellowfin_Cruises in the North Pacific Ocean from 2017-03-15 to 2017-12-15. These data include depth. The instruments used to collect these data include Mass Spectrometer and Niskin bottle. These data were collected by Sergio A. Sanudo-Wilhelmy of University of Southern California as part of the "The role of organic and metal cofactors on the biogenic synthesis of halogenated volatile hydrocarbons (Volatile_Hydrocarbons)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-01-04. The following is the text of the dataset description provided by BCO-DMO: Quantification of different chemical forms of cobalamin from SPOT cruises in 2017 Dataset Description: Acquisition Description: Samples for quantification of the different cobalamin forms were collected at a six of depths within the euphotic zone (5-250m). Seawater was collected from each CTD depth using Niskin bottles and immediately filtered. Cobalamins were analyzed as in 1 Suffridge et al. (2017). Two liters of seawater were filtered through µm pore-size filters and then preconcentrated using a C18 resin (HF Bondesil (Agilent Technologies) and analyzed by liquid chromatography/triple mass spectrometry (LC/MS/MS/MS). The LC-MS system consists of a ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography system. The LC system used a stable- bond C18 reversed-phase column (DiscoveryHSC18 10cm × 2.1mm,5 μm column, Supelco Analytical) with a100 μL sample loop.In order to increase the sensitivity and precision,theLC/MS was run in full-loop mode (100 μl/injection). The LC-MS system used for the pigment quantification consists of a Thermo TSQ Quantum Access electro-spray ionization triple quadruple mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography pump and auto-sampler. |
| Date received: | 20210104 |
| Start date: | 20170315 |
| End date: | 20171215 |
| Seanames: | North Pacific Ocean |
| West boundary: | -118.4 |
| East boundary: | -118.4 |
| North boundary: | 33.55 |
| South boundary: | 33.55 |
| Observation types: | |
| Instrument types: | mass spectrometer, Niskin bottle |
| Datatypes: | DEPTH - OBSERVATION |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | University of Southern California |
| Contributing projects: | |
| Platforms: | YELLOWFIN (31YE) |
| Number of observations: | |
| Supplementary information: | |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-04-27 17:10:08+00 |
| Editdate: | 2024-04-27 17:10:42+00 |