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OAS accession Detail for 0291636
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| accessions_id: | 0291636 | archive |
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| Title: | Growth rates across temperatures for 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017 (NCEI Accession 0291636) |
| Abstract: | This dataset contains biological and physical data collected from 2017-01-01 to 2018-10-31. These data include growth, taxon_code, and water temperature. The instruments used to collect these data include Turner Designs Fluorometer -10-AU. These data were collected by David A. Hutchins of University of Southern California as part of the "Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-04-27. The following is the text of the dataset description provided by BCO-DMO: Synechococcus growth rates Dataset Description: Growth rates across temperatures for 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017. |
| Date received: | 20200427 |
| Start date: | 20170101 |
| End date: | 20181031 |
| Seanames: | |
| West boundary: | -71.4007 |
| East boundary: | -71.2674 |
| North boundary: | 41.7129 |
| South boundary: | 41.4471 |
| Observation types: | biological, physical |
| Instrument types: | fluorometer |
| Datatypes: | growth rate, TAXONOMIC CODE, WATER TEMPERATURE |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | University of Southern California |
| Contributing projects: | |
| Platforms: | |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Natural seawater was enriched for photoautotrophs and split into multiple temperatures for two weeks. After the enrichment period, Synechococcus was isolated from each temperature. Each isolate's thermal niche was measured through a series of lab experiments and sequenced. Methods: Thermal niches were calculated by measuring each strain’s thermal performance curve. This was done by acclimating aliquots of each culture for two weeks to a broad range of temperatures between 9° and 33°. Temperatures >33° were added as needed for strains able to grow at these levels. This temperature range was chosen because it exceeds the current summer high and low temperatures in Narragansett Bay, and encompasses the projected warmer temperatures expected in coming decades. Strains were grown at each temperature in triplicate 8 ml borosilicate vials containing 5ml of F/2 medium. Biomass was recorded every two days using in vivo chlorophyll a fluorescence measured on a Turner AU-10 fluorometer (Turner Designs Inc., Sunnyvale, CA, USA), and growth rates and Eppley-Norberg thermal performance curves (Norberg 2004) were calculated in R (Team 2019) using the package growthTools (https://doi.org/10.5281/zenodo.3634918). Rare cultures containing contaminants (verified using fluorescence microscopy) were excluded from the dataset. In two strains for low temperature treatments, LA20 and LA27, after two weeks of acclimation no Synechococcus cells were observed in the culture, so the growth rate was set to zero for these cultures. The results of these thermal performance curves are hereafter referred to as a strain’s phenotype. |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-04-21 22:18:04+00 |
| Editdate: | 2024-04-21 22:18:26+00 |