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OAS accession Detail for 0291635
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| accessions_id: | 0291635 | archive |
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| Title: | Microbial enzyme activities: glucosidase and peptidase activities of bulk seawater samples from the RV\Sonne cruise SO248 in the South and North Pacific, along 180 W, May, 2016 (NCEI Accession 0291635) |
| Abstract: | This dataset contains data collected on R/V Sonne during cruise So248 from 2016-05-02 to 2016-05-30. These data include depth. The instruments used to collect these data include CTD profiler, Fluorometer, and Niskin bottle. These data were collected by Carol Arnosti of University of North Carolina at Chapel Hill as part of the "Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? (Patterns of activities)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-04-27. The following is the text of the dataset description provided by BCO-DMO: SO248: Bulk MCAMUF Dataset Description: This dataset includes MCAMUF (glucosidase and peptidase) hydrolysis rates to measure microbial enzyme activities in bulk (not filter-fractionated) seawater. Samples were collected on RV/Sonne cruise SO248 in May 2016. Links to archived CTD data are also provided. |
| Date received: | 20200427 |
| Start date: | 20160502 |
| End date: | 20160530 |
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| West boundary: | -180 |
| East boundary: | -179 |
| North boundary: | 58.9 |
| South boundary: | -30.0008 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD. Two substrates, -glucose and -glucose linked to a 4-methylumbelliferyl (MUF) fluorophore, were used to measure glucosidase activities. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and phenylalanine-serine-arginine (FSR) – were used to measure exo- and endo-acting peptidase activities, respectively. Hydrolysis rates of the substrates were measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005]. Incubations with the seven low molecular weight substrates were set up in a 96-well plate. For each substrate, triplicate wells were filled with a total volume of 200 L seawater for experimental incubations; triplicate wells were filled with 200 L autoclaved seawater for killed control incubations. Substrate was added at saturating concentrations. A saturation curve was determined with surface water from each station to determine saturating concentrations of substrate. The saturating concentration was identified as the lowest tested concentration of substrate at which additional substrate did not yield higher rates of hydrolysis. Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN spectrafluor plus; 360 nm excitation, 460 emission), with timepoints taken every 4-6 hours. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Scripts to calculate hydrolysis rates and produce the figures shown here are available in the associated Github repository [Hoarfrost, 2017]. L = substrate to measure leucine aminopeptidase (L-leucine-7-amido-4 MCA) AAF = substrate to measure chymotrypsin activity: ala-ala-phe-MCA AAPF = substrate to measure chymotrypsin activity: N-succinyl-ala-ala-pro-phe-MCA QAR = substrate to measure trypsin activity: Boc-gln-ala-arg-MCA FSR = substrate to measure trypsin activity: N-t-boc-phe-ser-arg-MCA |
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| Metadata version: | 1 |
| Keydate: | 2024-04-21 22:17:01+00 |
| Editdate: | 2024-04-21 22:17:20+00 |