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OAS accession Detail for 0291600
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| accessions_id: | 0291600 | archive |
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| Title: | Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - Chlorophyll, particulate organic carbon and particulate organic nitrogen from 2019-07-01 to 2019-08-31 (NCEI Accession 0291600) |
| Abstract: | This dataset contains biological, chemical, optical, and physical data collected from 2019-07-01 to 2019-08-31. These data include Carbon, Nitrogen, Partial pressure of CO2, chlorophyll a, irradiance, and water temperature. The instruments used to collect these data include CHN Elemental Analyzer, Cell Cultivator, and Fluorometer. These data were collected by Dr Edward Laws of Louisiana State University and Nigel D'Souza and Uta Passow of University of California-Santa Barbara as part of the "Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-08-31. The following is the text of the dataset description provided by BCO-DMO: Series 4A: POC, PON, chl-a Dataset Description: The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria Synechococcus elongatus CCMP1629 in a multifactorial design. This dataset contains measurements of extracted chlorophyll, particulate organic carbon (POC), and particulate organic nitrogen (PON) made over the course of the experiments. |
| Date received: | 20200831 |
| Start date: | 20190701 |
| End date: | 20190831 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Experimental setup: The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacterium S. elongatus CCMP1629 in a multifactorial design. Two CO2 concentrations were tested: 410 ppm, and 1000 ppm. For each CO2 concentration, four temperatures were tested: 20°C, 28°C, 36°C, and 44°C. Within each temperature, three light levels were tested: sub-optimum irradiance (SOI) intensity of 50 umol photons · m-2 · s-1, optimum irradiance (OI) intensity of 230 umol photons · m-2 · s-1 and extreme Irradiance (EI) intensity of 600 umol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at all four temperatures running simultaneously. This was repeated for each CO2 concentration. Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), and phosphate (PO4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson & Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol · kg−1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with the S. elongatus CCMP 1629 (SE1629) stock culture at the start of the experiments. Organic Carbon and Nitrogen concentrations Samples were filtered onto pre-combusted GF/F filters, dried at 60°C, and stored at room temperature until analyses of particulate organic carbon (POC), and particulate organic nitrogen (PON). Between 3 and > 10 mL were filtered, with larger filtration volumes used on the final day of the experiment. Samples were analyzed using an elemental analyzer (CEC 44OHA; Control Equipment). Samples where C or N concentrations were below instrument detection limits were flagged. Chlorophyll Daily subsamples from each treatment were filtered onto 0.45 um polycarbonate filters and stored at -20°C. Filters were placed in 90% acetone (v/v) overnight at -20°C, and the extracted chlorophyll was measured fluorometrically on a Turner 700 fluorometer (Strickland 1972). Chlorophyll-a liquid standards in 90% acetone (Turner Designs Inc.), and adjustable solid secondary standards (Turner Designs Inc. P/N 8000-952) were used for calibrations, and to calculate the chlorophyll content of the samples. |
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| Metadata version: | 1 |
| Keydate: | 2024-04-21 17:31:26+00 |
| Editdate: | 2024-04-21 17:31:45+00 |