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OAS accession Detail for 0291599
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| accessions_id: | 0291599 | archive |
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| Title: | Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms on 2019-10-23 (NCEI Accession 0291599) |
| Abstract: | This dataset contains biological, chemical, and survey - biological data collectedat Souther California Bight, Northeast Pacific Ocean on 2019-10-23. These data include dissolved Oxygen and species. The instruments used to collect these data include Oxygen Microelectrode Sensor. These data were collected by Dr Lisa A Levin and Nicholas Oesch of University of California-San Diego as part of the "Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae (Vision under hypoxia)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-11-01. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms. Please see additional datasets for this paper, including the “RetinalResponse TimeSeries” and the “Experiment Metrics” datasets. |
| Date received: | 20191101 |
| Start date: | 20191023 |
| End date: | 20191023 |
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| Observation types: | biological, chemical, survey - biological |
| Instrument types: | oxygen sensor |
| Datatypes: | DISSOLVED OXYGEN, SPECIES IDENTIFICATION |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | University of California - San Diego |
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| Supplementary information: | Acquisition Description: Detailed methods can be found in McCormick, LR; Levin, LA; Oesch, NW. 2019. Vision is highly sensitive to oxygen availability in marine invertebrate larvae. Journal of Experimental Biology 222, jeb200899. https://doi.org/10.1242/jeb.200899. Data shown in Figure 1, bottom panel. Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m − 2 s − 1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution. This dataset shows the three visual metrics that were calculated to quantify the effects of reduced pO2 on retinal function. V90, V50, and V10 were calculated for each individual larva as the oxygen where there was 90%, 50%, and 10% retinal function remaining, in respect to retinal responses in normoxia (surface-ocean oxygen levels). Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe. |
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| Metadata version: | 1 |
| Keydate: | 2024-04-21 17:30:25+00 |
| Editdate: | 2024-04-21 17:30:44+00 |