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OAS accession Detail for 0291573
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| accessions_id: | 0291573 | archive |
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| Title: | Record of d2H of dinosterol variability in down core lake sediments from Clear Lake, Palau on 2017-05-04 (NCEI Accession 0291573) |
| Abstract: | This dataset contains data collected on Small boats - CRRF during cruise Palau_lakes in the Philippine Sea on 2017-05-04. These data include depth below seafloor. The instruments used to collect these data include High Performance Liquid Chromatograph, Isotope-ratio Mass Spectrometer, and Mass Spectrometer. These data were collected by Julian P. Sachs and Julie N. Richey of University of Washington as part of the "Do Parallel Patterns Arise from Parallel Processes? (PaPaPro)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-08-02. The following is the text of the dataset description provided by BCO-DMO: Record of d2H of dinosterol variability in down core lake sediments Dataset Description: Record of d 2 H of dinosterol variability in down core lake sediments from Clear Lake, Palau. These data are published in the following journal article: Richey, J. N., & Sachs, J. P. (2016). Precipitation changes in the western tropical Pacific over the past millennium. Geology, 44(8), 671–674. https://doi.org/10.1130/g37822.1 |
| Date received: | 20190802 |
| Start date: | 20170504 |
| End date: | 20170504 |
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| West boundary: | 134.359 |
| East boundary: | 134.359 |
| North boundary: | 7.153 |
| South boundary: | 7.153 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Sampling and analytical procedures: Clear Lake sediments were extracted using a Dionex Accelerated Solvent Extractor (ASE 200), and the resulting total lipid extract was separated into neutral and polar fractions using column chromatography with aminopropyl gel as the stationary phase. The neutral fraction was then separated into hydrocarbon, wax ester, sterol and polar fractions via a silica gel column chromatography. Dinosterol was isolated then from the sterol fraction via reverse phase (RP)- high-performance liquid chromatography (HPLC) . Instruments: Dinosterol was isolated from the sterol fraction via reverse phase (RP)- high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an integrated autoinjector, quaternary pump, and fraction collector was coupled to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that was operated in positive atmospheric pressure chemical ionization (APCI+) mode. The HPLC method used is outlined in Nelson and Sachs (2013). Subsequently to HPLC separation the fraction containing dinosterol was analyzed via GC-MSD to verify sufficient baseline separation. Adjacent HPLC fractions were also analyzed to ensure that no dinosterol eluted into those fractions. After the dinosterol was sufficiently isolated from co-eluting compounds, the sample was injected onto a GC-irms for determination of the d 2 H of dinosterol. Hydrogen isotope determinations were made using a Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS) coupled to a Thermo Trace GC Ultra with a Varian VF-17ms FactorFour capillary column (60 m x 0.32 mm x 0.25 m) and a pyrolysis reactor. Samples were injected into a split/splitless inlet in splitless mode at 310 C. The oven temperature was ramped from 100 C to 220 C at a rate of 20 C/min, then at 2 C /min up to 325 C where it was held for 17 min. The carrier gas, He, was held constant at 2.6 mL/min. The pyroloysis reactor was maintained at 1400 C. Isotope values, expressed as D values, were calculated in Isodat software relative to VSMOW using a co-injection standard containing nC 28 nC 32 , nC 40 , and nC 44 of known ∂ 2 H values (obtained from Arndt Schimmelmann, Indiana University, Bloomington, IN, USA). The measured isotope values of dinosterol were corrected for the addition of hydrogen atoms (of known D value) that occurred during acetylation. Each sample was analyzed in at least duplicate, and error bars represent standard deviations of replicate measurements. |
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| Metadata version: | 1 |
| Keydate: | 2024-04-21 16:52:22+00 |
| Editdate: | 2024-04-21 16:53:40+00 |