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OAS accession Detail for 0291542
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| accessions_id: | 0291542 | archive |
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| Title: | FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018 (NCEI Accession 0291542) |
| Abstract: | This dataset contains biological, chemical, and physical data collected on R/V Endeavor during cruise EN616 in the New York Bight and North Atlantic Ocean from 2018-07-05 to 2018-07-13. These data include biomass carbon and depth. The instruments used to collect these data include Yokogawa Fluid Imaging Technologies FlowCam particle imaging system. These data were collected by David T. Drapeau, Jelena Godrijan, Stephen D. Archer, and William M. Balch of Bigelow Laboratory for Ocean Sciences as part of the "Coccolithophore Mixotrophy (Cocco-Mix)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-03-09. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: This dataset is part of a larger study with the following goals: Goal #1 : measure the mixotrophic uptake and assimilation of 14C-acetate, 14C-mannitol and 14C-glycerol as a carbon source by natural assemblages of coccolithophores and compare it to their autotrophic uptake and assimilation of DIC. These three organics were chosen due to their high potential for significant osmotrophy by coccolithophores as seen in previous culture studies (Godrijan et al. (2020) and BCO-DMO dataset: https://www.bco-dmo.org/dataset/858771 ). The design of these experiments used radiochemical and single cell/flow cytometer methods to distinguish osmotrophy of coccolithophores from that by other naturally-occurring microalgae. Goal #2 : test for the fixation of 14C-labeled organics into both POC and PIC fractions in natural populations of coccolithophores, in order to examine the potential role of coccolithophore osmotrophy in the biological carbon pump and alkalinity carbon pump paradigms. |
| Date received: | 20230309 |
| Start date: | 20180705 |
| End date: | 20180713 |
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| West boundary: | -72.927 |
| East boundary: | -66.517 |
| North boundary: | 43.718 |
| South boundary: | 36.986 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: At sea collections Nine stations were visited during R/V Endeavor EN616 cruise in the northwest Atlantic. At eight depths, three 10L Niskin samples were taken for discrete measurements of: 1. Chlorophyll, nutrients including nitrate, nitrite, ammonium, phosphate, and silicate 2. Particulate organic carbon (POC) plus particulate organic nitrogen (PON) 3. Particulate inorganic carbon (PIC) 4. Biogenic silica 5. Birefringence counts of coccolithophores (done ashore) 6. Shipboard Yokogawa Fluid Imaging Technologies FlowCam imaging cytometer, in order to enumerate the major microalgal classes and estimate the particle size distribution function Measurements 1 to 4 are part of BCO-DMO dataset 837074 (See https://www.bco-dmo.org/dataset/837074 , and the Related Datasets section below). Measurement 5 of birefringence counts data is BCO-DMO dataset 887863 (See https://www.bco-dmo.org/dataset/887863 , and the Related Datasets section below). Measurement 6 is flow cytometer data presented in this dataset. FlowCAM enumeration of various phytoplankton classes A Yokogowa FlowCAM imaging cytometer was used to enumerate different classes of phytoplankton. The instrument was keyed on particle backscattering and fluorescence properties. Samples were first filtered through 100um Nitex mesh to make sure the 100um diameter flow chamber did not clog. The instrument was run with a 10X objective in order to reliably count particles bigger than 4-5um diameter. Samples were processed according to Poulton and Martin (2010). Concentrations (per mL), percent contribution with respect to total particles, and biomass are presented. Carbon biomass was determined based on the Menden-Deuer & Lessard (2000) method. |
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| Metadata version: | 1 |
| Keydate: | 2024-04-21 14:09:03+00 |
| Editdate: | 2024-04-21 14:10:21+00 |