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OAS accession Detail for 0291527
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Title: Growth behaviors of diatom monoculture and diatom-bacteria co-cultures in the lab from 2021-11-01 to 2021-11-30 (NCEI Accession 0291527)
Abstract: This dataset contains biological, physical, and survey - biological data collected from 2021-11-01 to 2021-11-30. These data include density and species. The instruments used to collect these data include Flow Cytometer and Particle Size Analyzer. These data were collected by Kimberly Halsey and Stephen Giovannoni of Oregon State University as part of the "Interactions between phytoplankton and bacterioplankton mediated by volatile organic compounds (Plankton Interactions and VOC)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-12-16.

The following is the text of the dataset description provided by BCO-DMO:

Acquisition Description:
This dataset represents the growth behaviors of diatom ( Phaeodactylum tricornutum ) monoculture and diatom-bacteria co-cultures in the lab.

The cultures described in the dataset were grown as follows:
Cultures were inoculated in f/2+Si media and grown under 60 micro-einsteins, 12 hours light:12 hour dark cycles at 19 degrees Celsius. Sampling for VOCs was done in triplicate during exponential and stationary growth phases. Exponential phase sampling was done when the cell density of P. tricornutum was 2-4 * 10^5 per milliliter (ml) and at 6-7 hours after dawn. Stationary phase sampling was done when the cell density of P. tricornutum was 2-4 * 10^6 per milliliter (ml) at 6-7 hours after dawn.

Cell densities were measured by a particle counter (Coulter) or Guava flow cytometry.
Date received: 20221216
Start date: 20211101
End date: 20211130
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Submitting institution: Biological and Chemical Oceanography Data Management Office
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Metadata version: 1
Keydate: 2024-04-21 13:45:55+00
Editdate: 2024-04-21 13:46:22+00