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OAS accession Detail for 0291404
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| accessions_id: | 0291404 | archive |
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| Title: | Supplementary Table 3B: Replicate cell counts for the 11 samples and alkaline phosphatase activity measurements available for any of the 11 samples from 2015-11-30 to 2016-01-30 (NCEI Accession 0291404) |
| Abstract: | This dataset contains data collected on R/V JOIDES Resolution during cruise IODP-360 in the Indian Ocean from 2015-11-30 to 2016-01-30. These data include depth. The instruments used to collect these data include Microscope-Fluorescence, plate reader, and ultrasonic cell disrupter. These data were collected by Virginia P. Edgcomb of Woods Hole Oceanographic Institution as part of the "Collaborative Research: Delineating The Microbial Diversity and Cross-domain Interactions in The Uncharted Subseafloor Lower Crust Using Meta-omics and Culturing Approaches (Subseafloor Lower Crust Microbiology)" project and "International Ocean Discovery Program (IODP)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-07-08. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Supplementary Table 3B: Overview of archaeal and bacterial lipid biomarkers and cell counts. Replicate cell counts for the 11 samples and alkaline phosphatase activity measurements available for any of the 11 samples. Samples were taken on board of the JOIDES Resolution between November 30, 2015 and January 30, 2016 in the SW Indian Ridge. |
| Date received: | 20200708 |
| Start date: | 20151130 |
| End date: | 20160130 |
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| West boundary: | 57.278 |
| East boundary: | 57.278 |
| North boundary: | -32.706 |
| South boundary: | -32.706 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Alkaline phosphatase (AP) activity was measured using the fluorogenic substrate 4-methylumbelliferyl phosphate (MUF-P) (Sigma-Aldrich, St. Louis, MO) and its reference standard, methylumbelliferone (MUF). Fluorescence was measured using black, flat bottom, 96-well microplates in a Spark 10M Multimode Microplate Reader (Tecan, Männedorf, Switzerland). Fluorescence of MUF is greatest at pH 10, therefore 25 μL of 0.4 M NaOH was added to the wells (final concentration 40 mM) to be read. 25 μL of 1M EDTA was added as well (100 mM final concentration) to prevent precipitation of c 439 arbonate from sampled veins. Fluorescence was measured with an excitation wavelength of 380 nm and emission of 454 nm for all substrates and standards. One cm 3 powdered rock was mixed with 5 cm 3 of sterileartificial seawater (ASW) in a 8 mL serum vial with 90:5:5 N2:CO2:H2 headspace. 700 μL of each slurry was withdrawn with a sterile syringe to a 1.5 mL Eppendorf tube after setup but before sealing the vial; this sample served as T0, with triplicate 200 μL technical replicates. These 700 μL samples were briefly centrifuged (60 sec. at 2500 rpm) and the supernatant used for the T0 analyses. Two additional samples were taken using the same methods as for T0 after at least 2 weeks and then again after 4-6 weeks to generate a slope of activity. Incubations were kept at 10˚C, the inferred in situ temperature, for the duration of each assay. Autoclaved, powderized rock from each of the samples was tested to determine the amount of fluorophore adsorbance to rock powder. Adsorbance was found to behave in a systematic manner, resulting in a straight line when comparing fluorescence standards in artificial seawater (ASW) alone with fluorescence standards plus rock powder in ASW, although this relationship was found to be different when measured at 4 hours versus days later. Therefore, a correction factor for adsorbance was applied to the enzyme data for the initial measurement (t0, y=1.90x-676), taken |
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| Metadata version: | 1 |
| Keydate: | 2024-04-20 16:08:43+00 |
| Editdate: | 2024-04-20 16:09:37+00 |