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OAS accession Detail for 0291399
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| accessions_id: | 0291399 | archive |
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| Title: | Bottle sample data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project) (NCEI Accession 0291399) |
| Abstract: | This dataset contains biological, chemical, optical, and physical data collected on R/V Hugh R. Sharp during cruise HRS1610 from 2016-08-07 to 2016-08-17. These data include Ammonium, Nitrate, Nitrite, density, depth, fluorescence, phosphate, salinity, water pressure, and water temperature. The instruments used to collect these data include High-Performance Liquid Chromatograph, Niskin bottle, Nutrient Autoanalyzer, Pump, and Spectrometer. These data were collected by Margaret Mulholland of Old Dominion University as part of the "Cyanate in the Sea: Sources, Sinks, and Quantitative Significance (CyanateInTheSea)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2024-03-19. The following is the text of the dataset description provided by BCO-DMO: Bottle sample data from CTD casts Dataset Description: Methods and Sampling: Nutrient samples at each depth were collected directly from Niskin bottles through a 0.2 μm capsule filter (Pall Supor ® ) using a peristaltic pump at pressures ≤5 mm Hg. Filtrate was collected directly into two 50 mL sterile polypropylene centrifuge tubes (Falcon ® ) to measure nitrate (NO 3 – ), nitrite (NO 2 – ), orthophosphate ions (simplified as PO 4 , sum of HPO 4 2– and PO 4 3– ), and urea concentrations. Filtrate was also pumped into three 2 mL sterile polypropylene tubes for cyanate determinations, two 15 mL OPA-treated polypropylene tubes (Falcon ® ) for ammonium (NH 4 + ) analyses, and two 40 mL combusted amber glass vials for analysis of total dissolved free primary amine. To minimize contamination from filtration and between samples, filters and tubing were rinsed thoroughly with site water before sample collection. NO 3 – + NO 2 – , NO 2 – , PO 4 , and urea samples (duplicates) were stored at 4 °C until analysis within 48 hours of their collection using a nutrient autoanalyzer (Astoria-Pacific, Inc., USA) according to the manufacturer’s specifications. The method detection limits for NO 3 – + NO 2 – , NO 2 – , PO 4 , and urea were 0.14 μmol L –1 , 0.07 μmol L –1 , 0.03 μmol L –1 , and 0.08 μmol L –1 , respectively. NH 4 + samples (duplicates) were kept at 4 °C until analysis within 24 hours of collection. The concentrations of NH 4 + were measured onboard using the OPA-fluorescence method of Holmes et al. (1999) with a spectrofluorometer. The method detection limit was 10.0 nmol L –1 . Cyanate samples (triplicates) were stored in liquid nitrogen aboard the ship and at -80 °C once samples were returned to the land-based laboratory. Cyanate concentrations were measured by high-performance liquid chromatography (HPLC) using a precolumn fluorescence derivatization method (Widner et al., 2013; Widner and Mulholland, 2017). The method detection limit was 0.4 nmol L –1 . |
| Date received: | 20240319 |
| Start date: | 20160807 |
| End date: | 20160817 |
| Seanames: | |
| West boundary: | -78.5773 |
| East boundary: | -73.3663 |
| North boundary: | 37.668 |
| South boundary: | 33.334 |
| Observation types: | biological, chemical, optical, physical |
| Instrument types: | chromatograph, Flow-through pump, mass spectrometer, Niskin bottle, nutrient autoanalyzer |
| Datatypes: | AMMONIUM (NH4), FLUORESCENCE, HYDROSTATIC PRESSURE, NITRATE, NITRITE, phosphate, SALINITY, WATER DENSITY, WATER TEMPERATURE |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Old Dominion University |
| Contributing projects: | |
| Platforms: | Hugh R. Sharp (33H5) |
| Number of observations: | |
| Supplementary information: | |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-04-20 16:01:45+00 |
| Editdate: | 2024-04-20 16:02:21+00 |