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OAS accession Detail for 0291389
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| accessions_id: | 0291389 | archive |
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| Title: | Experimental results: summary of microcosm data from Dauphin Island Cubitainer Experiment (DICE) from samples collected by R/V E.O. Wilson in the Gulf of Mexico, Alabama (En-Gen DMSP Cycling project) from 2006-10-01 to 2006-10-31 (NCEI Accession 0291389) |
| Abstract: | This dataset contains biological data collected on R/V E.O. Wilson during cruise DMSP_Dauphin_Island from 2006-10-01 to 2006-10-31. These data include chlorophyll-a; fluorometric method. The instruments used to collect these data include Turner Designs 700 Laboratory Fluorometer and bucket. These data were collected by Ronald Kiene of Dauphin Island Sea Lab and Mary Ann Moran and William Whitman of University of Georgia as part of the "En-Gen: A Functional Genomics Approach to Organic Sulfur Cycling in the Ocean (En-Gen DMSP Cycling)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-11-01. The following is the text of the dataset description provided by BCO-DMO: Summary of microcosm data from Dauphin Island Cubitainer Experiment (DICE). Dataset Description: Summary of parameters measured in experimental and control microcosms as part of the Dauphin Island Cubitainer Experiment (DICE). Experimental design, methods, and results are further described in Howard et al. (2010) and Rinta-Kanto et al. (2011). |
| Date received: | 20191101 |
| Start date: | 20061001 |
| End date: | 20061031 |
| Seanames: | |
| West boundary: | -87.99513 |
| East boundary: | -87.99513 |
| North boundary: | 30.05068 |
| South boundary: | 30.05068 |
| Observation types: | biological |
| Instrument types: | bucket, fluorometer |
| Datatypes: | CHLOROPHYLL A |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Dauphin Island Sea Lab, University of Georgia |
| Contributing projects: | |
| Platforms: | |
| Number of observations: | |
| Supplementary information: | Acquisition Description: See Howard et al. 2011 and Rinta-Kanto et al. 2011 for detailed methods, summarized below: "In October 2006, seawater was collected from surface waters ( Three microcosms were amended with 10 um sodium nitrate (NaNO3) and 0.6 um potassium phosphate (K2HPO4) to serve as the experimental microcosms. Three microcosms were left untreated to serve as the control. The Cubitainers were maintained at 27 degrees C on a 12-hour light/dark cycle for the duration of the experiment. Chemical and activity measurements were collected from the microcosms at the beginning of the experiment (Day 0) and every day for the duration of the experiment at the same time. Chlorophyll-a samples were collected by filtration on Whatman GF/F fiters. The filters were extracted in 90% acetone for 24 hours at -20 degrees C. The extracts were quantified by fluorometry on a Turner Designs TD-700. Samples for dissolved dimethylsulfoniopropionate (DMSPd) were collected by filtration through GF/F filters. Total dimethylsulfoniopropionate (DMSP), dissolved + particulate, was measured by acidifying whole seawater with 5 ul per ml of 50% H2SO4. Dissolved and total DMSP were quantified as dimethylsulfide (DMS) after alkaline hydrolysis. Bacterial production was measured by [3H]leucine incorporation. Rates of DMSPd consumption were determined by multiplying the DMSPd concentrations by their respective DMSPd loss rate constant from parallel samples. DMSPd consumption rate constants were determined by measuring the loss of tracer levels of [35S]-DMSP from the dissolved ( References: Vila-Costa, M., Kiene, R.P., and Simó, R. (2008) Seasonal variability of the dynamics of dimethylated sulfur compounds in a coastal northwest mediterranean site. Limnol Oceanogr 53: 198–211. |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-04-20 15:46:15+00 |
| Editdate: | 2024-04-20 15:46:56+00 |