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OAS accession Detail for 0291386
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| accessions_id: | 0291386 | archive |
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| Title: | Chlorophyll and phaeopigment concentrations from incubation experiments performed with amended Southern Drake Passage on RVIB Nathaniel B. Palmer cruise NBP 16-08 from September to October 2016 (NCEI Accession 0291386) |
| Abstract: | This dataset contains biological data collected on RVIB Nathaniel B. Palmer during cruise NBP1608 from 2016-09-12 to 2016-10-09. These data include chlorophyll-a; fluorometric method. The instruments used to collect these data include Turner Designs Fluorometer 10-AU. These data were collected by Phoebe Dreux Chappell of Old Dominion University, Bethany D. Jenkins of University of Rhode Island, and Kristen Buck of University of South Florida as part of the "Collaborative Research: Investigating Iron-binding Ligands in Southern Ocean Diatom Communities: The Role of Diatom-Bacteria Associations (Diatom_Bacteria_Ligands)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-03-18. The following is the text of the dataset description provided by BCO-DMO: Chlorophyll and phaeopigment concentrations from incubation experiments performed with amended Southern Drake Passage waters on cruise NBP 16-08 Dataset Description: Chlorophyll and phaeopigment concentrations from incubation experiments performed with amended Southern Drake Passage waters on cruise NBP 16-08. |
| Date received: | 20220318 |
| Start date: | 20160912 |
| End date: | 20161009 |
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| West boundary: | -64.6485 |
| East boundary: | -59.565 |
| North boundary: | -62.332 |
| South boundary: | -62.46 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Incubation 1: Chlorophyll and phaeopigment samples were collected from an incubation experiment set up with near-surface waters (35 - 25 meters depth) collected using the TMC CTD at -62.332N, -64.6485E over three casts with each cast homogenized in 50 L carboys. Incubation setup occurred in a TMC van and treatments were incubated in 4L polycarbonate bottles in a temperature controlled (2 °C) lighted incubator van with 24hr light. Sampling occurred in a TMC bubble.Treatment identifiers are as follows: Cast = Homogenized carboy sample at T0 for individual casts. A = control B = +1 nM 57 FeCl 3 C = +4 nM Fe 57 FeCl 3 D = +10 nM Fe 57 FeCl 3 E = +600 pM Vitamin B 12 F = +4 nM 57 FeCl 3 /+600 pM Vitamin B 12 G = Dark control H = Dark +1 nM 57 FeCl 3 I = Dark +4 nM Fe 57 FeCl 3 J = Dark +10 nM Fe 57 FeCl 3 K = Dark +600 pM Vitamin B 12 L = Dark +4 nM 57 FeCl 3 /+600 pM Vitamin B 12 Incubation 2: Chlorophyll and phaeopigment samples were collected from an incubation experiment set up with near-surface waters (35 - 25 meters depth) collected using the TMC CTD at -62.46N, -59.565E over two casts with each cast homogenized in 50 L carboys. Incubation setup occurred in a TMC van, treatments were incubated in 4L polycarbonate bottles in a temperature controlled (2 °C) lighted incubator van with 24hr light. Sampling occurred in a TMC bubble. Treatment identifiers are as follows: Cast = Homogenized carboy sample at T0 for individual casts. M = control N = +4 nM Fe 57 FeCl 3 O = +600 pM Vitamin B 12 S = Dark control T = Dark +4 nM Fe 57 FeCl 3 U = Dark +600 pM Vitamin B 12 Incubation 3: Chlorophyll and phaeopigment samples were collected from an incubation experiment set up with near-surface waters (35 - 25 meters depth) collected using the TMC CTD at -62.332N, -64.6485E over a single cast where waters were homogenized in 50 L carboys. The only amendment included adding 50% of 0.2 um filtered near surface (35 – 25 meters depth) waters collected at -62.46N, -59.565E. Incubation setup occurred in a TMC van and treatments were incubated in 4L polycarbonate bottles in a temperature controlled (2 °C) lighted incubator van with 24hr light. Sampling occurred in a TMC bubble. Treatment identifiers are as follows: Cast = Homogenized carboy sample at T0 for individual casts. Q = control (unfiltered water from -62.332N, -64.6485E) R = 50% unfiltered water from -62.332,N -64.6485E with 50% 0.2 um filtered waters from -62.46N, -59.565E For each sampling timepoint, samples of 50-100 mL were filtered onto 25 mm GFF filters and extracted using the protocol of: Jespersen, A.M. and Christoffersen, K. (1987) Measurements of Chlorophyll-a from phytoplankton using ethanol as extraction solvent. Arch. Hydrobiol. 109 (3) 445-454, as updated in: Morison, F. and Menden-Deuer, S. (2015) Early spring phytoplankton dynamics in the sub polar North Atlantic: The influence of protistan herbivory. Limnol. Oceanogr. 60(4) 1298-1313. Analyses were performed by Ms. Zuzanna Abdala (ODU) and Ms. Alexa Sterling (URI). At the start of the cruise, Turner 10-AU Fluorometer was calibrated using Chla Standard Stock Solution (SSS) from Anacystis nidulans algae (#C-5753) following the protocol by Kirk Ireson & Karen Baker (PDF). Calibration was performed by Dr. Randelle Bundy (UW) and Dr. Bethany Jenkins (URI). |
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| Metadata version: | 1 |
| Keydate: | 2024-04-20 15:40:47+00 |
| Editdate: | 2024-04-20 15:41:54+00 |