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OAS accession Detail for 0278868
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Title: 16S rRNA gene (from DNA) from samples collected on cruise Chikyu-331 in the Okinawa Trough, Japan from September to October 2010 (NCEI Accession 0278868)
Abstract: This dataset contains physical data collected on Chikyu during cruise Chikyu-331 in the East China Sea (Tung Hai) from 2010-09-16 to 2010-09-27. These data include water temperature. The instruments used to collect these data include Piston Corer. These data were collected by Christopher House and Leah Brandt of Pennsylvania State University as part of the "An In-Depth analysis of the subvent biosphere within Okinawa Backarc Basin (IODP 331, Iheya North Hydrothermal Field) sediments (Subvent_Biosphere_Sediments)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" and "International Ocean Discovery Program (IODP)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-11-22.

The following is the text of the dataset description provided by BCO-DMO:

16S rRNA gene (from DNA)

Dataset Description:
Next-generation sequencing data across a hydrothermal gradient; Okinawa Backarc Basin IODP 331 – subvent biosphere. The purpose of this dataset was to investigate taxonomic changes via the 16S rRNA gene from extractable DNA across 45 meters of recovered core. The temperature gradient in this section of the dynamic Iheya North Hydrothermal system was estimated to be ~3C/m. The interval transitions sharply from low-temperature marine mud to hydrothermally altered clay.
Date received: 20191122
Start date: 20100916
End date: 20100927
Seanames:
West boundary: 126.892
East boundary: 126.901
North boundary: 27.794
South boundary: 27.79
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Instrument types:
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Submitter:
Submitting institution: Biological and Chemical Oceanography Data Management Office
Collecting institutions:
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Number of observations:
Supplementary information: Acquisition Description:
Sampling of cores was done in accordance with IODP protocols.

All C0014 samples were cored on 16 September 2010 via via HPCS (hydraulic piston coring system).
All C0015 samples were cored on 18 September 2010 via HPCS.
All C0017 samples were cored on 27 September 2010 via HPCS.

Core sections were kept frozen (-80°C) until DNA extractions. A MoBio® Power Soil kit was used to extract environmental DNA. See publication below for sequencing and analysis methodologies.
Availability date:
Metadata version: 1
Keydate: 2023-05-28 04:56:37+00
Editdate: 2023-05-28 04:57:21+00